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BioControl

, Volume 60, Issue 2, pp 281–292 | Cite as

Persistent fungal root endophytes isolated from a wild barley species suppress seed-borne infections in a barley cultivar

  • Brian R. MurphyEmail author
  • Fiona M. Doohan
  • Trevor R. Hodkinson
Article

Abstract

Ten fungal root endophytes were isolated from wild populations of Hordeum murinum ssp. murinum L. and inoculated onto untreated seeds of a barley cultivar using five artificial and one soil-based growth media. A co-inoculant of all ten isolates as well as two individual isolates successfully suppressed the development of seed-borne fungal infections on germinated and ungerminated seed. The two most successful isolates were also the most persistent as re-emergents and may provide real potential for development as crop inoculants. All isolates were more persistent in barley exposed to light after germination. The soil-based compost was associated with the greatest degree of seed-borne infection suppression, and the most successful artificial medium for suppressing seed-borne infections was also the medium with the most similar pH to the soil at the sampling sites. These results suggest a direct antagonistic effect of the fungal isolates on seed-borne pathogens without the induction of plant defences.

Keywords

Fungal root endophytes Hordeum murinum ssp. murinum L. Biological control Seed-borne infections 

Notes

Acknowledgments

We thank: Goldcrop Seeds, Cork, Ireland for the generous supply of barley seeds, and for advice on suitable cultivars to use; Helena Murphy for proof reading and the de-cluttering of technical terms; laboratory technicians at Trinity College Dublin for providing supplies and technical support. Trinity College Dublin provided financial support through a PhD studentship grant.

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Copyright information

© International Organization for Biological Control (IOBC) 2014

Authors and Affiliations

  • Brian R. Murphy
    • 1
    Email author
  • Fiona M. Doohan
    • 2
  • Trevor R. Hodkinson
    • 1
  1. 1.School of Natural Sciences & Trinity Centre for Biodiversity ResearchTrinity College DublinDublin 2Ireland
  2. 2.UCD Earth Institute and School of Biology & Environmental ScienceUniversity College DublinDublin 4Ireland

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