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Relationship between endospore viability and insecticidal potency of Bacillus thuringiensis subsp. aizawai NT0423

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Abstract

Bioassay can be used for analysis of the biological potency of Bacillus thuringiensis (Bt) in fermentation and formulation but requires precise scheduling and several repetitions. Alternatively, this work explored if the endospore counting could be used to predict the potency of Bt technical powder. Analyses of Bt technical powers provided a strong linear relationship (r = 0.971) between the number of viable endospores and the potency of the technical powder against second instar Plutella xylostella (L.) larvae. Next, a Bt wettable powder formulation was stored at 25 and 40 °C for 12 weeks to investigate the influence of storage temperature on the prediction of insecticidal potency based on the counting. At 25 °C storage, the insecticidal potency could be predicted based on the counting, but at 40 °C the predicted insecticidal potency was much lower than the measured potency. These results suggest that the NT0423 endospore viability can be used to predict its potency in production, but the relationship may not be the same following the storage at high temperature.

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Acknowledgments

We are grateful to Dr. Bong-Jin Chung in Dongbu Hannong Co (Republic of Korea) for assistance with Bt analyses and Dr. Dong-Soo Yang (Abson BCL Inc., Republic of Korea) for technical assistance in the fermentation and spray-drying of Bt NT0423. This research was supported by Bio-industry Technology Development Program, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.

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Authors and Affiliations

  1. Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju, 561-756, Republic of Korea

    Jae Su Kim & Se Jin Lee

  2. Entomology Research Laboratory, University of Vermont, Burlington, VT, 05405-0105, USA

    Margaret Skinner & Bruce L. Parker

  3. Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, 570 752, Republic of Korea

    Byung-Taek Oh & Kui-Jae Lee

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  1. Jae Su Kim
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  2. Se Jin Lee
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  3. Margaret Skinner
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  4. Bruce L. Parker
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  5. Byung-Taek Oh
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  6. Kui-Jae Lee
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Correspondence to Jae Su Kim.

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Handling Editor: Helen Roy

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Kim, J.S., Lee, S.J., Skinner, M. et al. Relationship between endospore viability and insecticidal potency of Bacillus thuringiensis subsp. aizawai NT0423. BioControl 58, 607–614 (2013). https://doi.org/10.1007/s10526-013-9527-x

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  • DOI: https://doi.org/10.1007/s10526-013-9527-x

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