, Volume 58, Issue 4, pp 543–552 | Cite as

Development of the predator Eocanthecona furcellata on different proportions of nucleopolyhedrovirus infected Spodoptera litura larvae and potential for predator dissemination of virus in the field

  • R. K. Gupta
  • Mudasir GaniEmail author
  • P. Jasrotia
  • K. Srivastava


The present work aimed to determine the impact of nucleopolyhedrovirus (NPV) on the development of Eocanthecona furcellata Wolff and to measure the infectivity of NPV discharged through its faeces. Developmental time, survival rate, sex ratio and incubation period of the predator reared on different proportions of healthy and NPV infected S. litura larvae as lifetime meal did not vary significantly. However, when the proportion of infected prey in the predator’s lifetime meal exceeded 50 % significant reductions in body weight, fecundity, longevity and percent egg hatchability were noticed. The virus did not show detrimental effect on the developmental biology of the predator in the subsequent generations when offspring emerging from nymphs fed only on virus infected prey throughout their life were reared on 50 % of infected prey. Therefore, adverse effects on E. furcellata are unlikely in the field as the predator is not expected to consume more than 50 % of infected prey even if the virus application is done at recommended dose. Under field conditions, it was found that increased proportions of infected prey enhanced the dissemination of viral inoculum through the faeces of the predators and the subsequent infection in S. litura larvae ranged from 22 to 52 %.


Biological control Bioinsecticides Entomopathogens Pentatomidae 



The authors are grateful to Dr. R. J. Rabindra for suggestions and guidance during the course of investigation and to the Director of Research, SKUAST-Jammu for support. Our sincere thanks are also due to the Department of Science and Technology, Government of India for funding and to the National Bureau of Agriculturally Important Insects (NBAII) for encouragement and co-operation.


  1. Aganon TM, Romero ES (2008) Utilization of predatory stinkbug E. furcellata (Wolff) against tomato fruitworm Helicoverpa armigera (Hubner) and eggplant fruit and shootborer Leucinodes orbonalis (Guenee). CLSU R D J 2(1):100–108Google Scholar
  2. Beekman AG (1980) The infectivity of polyhedra of nuclear polyhedrosis virus (N.P.V.) after passage through the gut of an insect predator. Experientia 36:858–859CrossRefGoogle Scholar
  3. Biever KD, Andrews PL, Andrews PA (1982) Use of predator, Podisus maculiventris, to distribute virus and initiate epizootics. J Econ Entomol 75:150–152Google Scholar
  4. Capinera JL, Barbosa P (1975) Transmission of a nuclear polyhedrosis virus to gypsy moth larvae by Calosoma sycuphanta. Ann Entomol Soc Am 68:593–594Google Scholar
  5. Castillejos V, Garcia L, Cisneros J, Goulson D, Ronald D, Cave PC, Williams T (2001) The potential of Chrysoperla rufilabris and Doru taeniatum as agents for dispersal of S. frugiperda nucleopolyhedrovirus in maize. Entomol Exp Appl 98:353–359CrossRefGoogle Scholar
  6. Chu Y (1975) Rearing density of E. furcellata with special consideration to its mass production (Asopinae: Pentatomidae). Rostria 24:135–140Google Scholar
  7. Cossentine JE, Lewis LC (1988) Impact of Nosema pyrausta, Nosema sp. and a nuclear polyhedrosis virus on Lydella thompsoni within infected Ostrinia nubilalis hosts. J Invert Pathol 51:126–132CrossRefGoogle Scholar
  8. Entwistle PF, Forkner AC, Green BM, Cory JS (1993) Avian dispersal of nuclear polyhedrosis viruses after induced epizootics in the pine beauty moth, Panolis flammea (Lepidoptera: Noctuidae). Biol Control 3:61–69CrossRefGoogle Scholar
  9. Fuxa JR, Richter AR (1994) Distance and rate of spread of Anticarsia gemmatalis (Lepidoptera: Noctuidae) nuclear polyhedrosis virus released into soybean. Environ Entomol 23:1308–1316Google Scholar
  10. Gupta RK, Khan MS, Bali K, Monobrullah M, Bhagat RM (2004) Predatory bugs of Zygogramma bicolorata Pallister: an exotic beetle for biological suppression of Parthenium hysterophorus L. Curr Sci 87:1005–1010Google Scholar
  11. Gupta RK, Amin M, Bali K, Monobrullah M, Jasrotia P (2010) Vertical transmission of sub lethal granulovirus infection in the tobacco caterpillar S. litura. Phytoparasitica 38:209–216CrossRefGoogle Scholar
  12. Kaya HK (1982) Parasites and predators as vectors of insect diseases. In: proceedings of the 3rd international colloquium on invertebrate pathology, Brighton, UK, pp 39–44Google Scholar
  13. Mcfarlane JE (1985) Nutrition and digestive organs. In: Blum (ed) Fundamentals of insect physiology. Wiley-Interscience, New York, USA, pp 59–89Google Scholar
  14. Monobrullah M, Shankar U, Bharti P, Gupta RK, Kaul V (2007) Effect of host plant on the infectivity of SlMNPV to S. litura (Fabricius) (Lepidoptera: Noctuidae) larvae. J Asia-Pacific Entomol 10:151–155CrossRefGoogle Scholar
  15. Moscardi F (1999) Assessment of the application of baculoviruses for control of Lepidoptera. Annu Rev Entomol 44:257–289PubMedCrossRefGoogle Scholar
  16. Moscardi F, De Souza ML, de Batista Castro ME, Moscardi ML, Szewczyk B (2011) Baculovirus pesticides: present state and future perspectives. In: Ahmad I, Ahmad F, Pitchel J (eds) Microbes and microbial technology. Springer, New York, USA, pp 415–446CrossRefGoogle Scholar
  17. Nyunt KT (2001) Impact of planting dates on the population of cotton pests and natural enemies in Myanmar. M.Sc. agr. thesis at Georg-August University, Goettingen, Germany, 99 ppGoogle Scholar
  18. Nyunt KT (2008) Potential of the predatory pentatomid Eocanthecona furcellata (Wolff) as a biocontrol agent on American bollworm in cotton in Myanmar. Ph.D. agr. thesis at Georg-August University, Goettingen, Germany, 11 ppGoogle Scholar
  19. Richards A, Cory J, Speight M, Williams T (1999) Foraging in a pathogen reservoir can lead to local host population extinction: a case study of a Lepidoptera-virus interaction. Oecologia 118:29–38PubMedCrossRefGoogle Scholar
  20. Rothman LD, Myers JH (1994) Nuclear polyhedrosis virus treatment effect on reproductive potential of western tent caterpillar (Lepidoptera: Lasiocampidae). Environ Entomol 23:864–869Google Scholar
  21. Ruberson JR, Young SY, Kring TJ (1991) Suitability of prey infected by nuclear polyhedrosis virus for development, survival, and reproduction of the predator Nabis roseipennis (Heteroptera: Nabidae). Environ Entomol 20:1475–1479Google Scholar
  22. Sait SM, Begon M, Thompson DJ, Harvey JA (1996) Parasitism of baculovirus-infected Plodia interpunctella by Venturia canescens and subsequent virus transmission. Funct Ecol 10:586–591CrossRefGoogle Scholar
  23. Sajap AS (1989) Impact of Nosema pyrausta (Microsporida: Nosematidae) on a predator, Chrysoperla carnea (Neuroptera: Chrysopidae). Environ Entomol 18:172–176Google Scholar
  24. Sajap AS, Lewis LC (1988) Effects of the microsporidium Nosema pyrausta (Microsporida: Nosematidae) on the egg parasitoid, Trichogramma nubilale (Hymenoptera: Trichogrammatidae). J Invert Pathol 52:294–300CrossRefGoogle Scholar
  25. Sajap AS, Kotulai JR, Kadir HA, Hussein MY (1999) Impact of prey infected by nuclear polyhedrosis virus on a predator, Sycanus leucomesus walk. (Hemiptera: Reduviidae). J Appl Entomol 123:93–97CrossRefGoogle Scholar
  26. Shorey MM, Hale LL (1965) Mass rearing of the larvae of nine noctuid species on a simple artificial medium. J Econ Entomol 58:522–524Google Scholar
  27. Siri N (2007) Augmentative biological control in Thailand: current status and future challenge. In: the 5th international symposium on biocontrol and biotechnology at Khon Kaen University, Nong Khai Campus, Nong Khai, Thailand, p 8Google Scholar
  28. Smirnoff WA (1959) Predators of Neodiprion swainei Midd (Hymenoptera: Tenthredinidae) larval vectors of virus diseases. Can Entomol 91:246–248CrossRefGoogle Scholar
  29. Stairs GR (1966) Transmission of virus in tent caterpillar populations. Can Entomol 98:1100–1104CrossRefGoogle Scholar
  30. Suasa-ard W (1988) Utilization of Eocanthecona furcellata Wolff (Hemiptera: Pentatomidae) for augmentative biological control of semilooper, Ophiusa janata L. (Lepidoptera: Noctuidae), in Thailand. In: symposium on biological control of pests in tropical agricultural ecosystems, pp 191–201. BIOTROP special publication no. 36, SEAMEO-BIOTROP, Bogor, Indonesia, 349 ppGoogle Scholar
  31. Szewczyk B, Hoyos-Carvajal L, Paluszek M, Skrzecz I, Lobo de Souza M (2006) Baculoviruses-re-emerging biopesticides. Biotechnol Adv 24:143–160PubMedCrossRefGoogle Scholar
  32. Szewczyk B, Rabalski L, Krol E, Sihler W, Souza ML (2009) Baculovirus biopesticides—a safe alternative to chemical protection of plants. J Biopes 2(2):209–216Google Scholar
  33. Vasconcelos SD, Williams T, Hails RS, Cory JS (1996) Prey selection and baculovirus dissemination by carabid predators of Lepidoptera. Ecol Entomol 21:98–104CrossRefGoogle Scholar
  34. Vickerman DB, Trumble JT (2003) Biotransfer of selenium: effects on an insect predator, Podisus maculiventris. Ecotoxicology 12:497–504PubMedCrossRefGoogle Scholar
  35. Yasuda T, Wakamura S (1992) Rearing of the predatory stink bug E. furcellata (Wolff) (Hemiptera: Pentatomidae), on frozen larvae of S. litura (Fabricius) (Lepidoptera: Noctuidae). Appl Entomol Zool 27:303–305Google Scholar
  36. Yi NN, Kyi W (2000) Biological control of cotton bollworm and chickpea pod borer, Helicoverpa armigera using predator E. furcellata and parasitoid Campoletis chlorideae. In: proceeding of the annual research conference, April 3–5, Yangon, Myanmar, 58–74 ppGoogle Scholar
  37. Young OP, Hamm JJ (1985) The effect of the consumption of NPV-infected dead fall armyworm larvae on the longevity of two species of scavenger beetles. J Entomol Sci 20:90–94Google Scholar
  38. Young SY, Yearian WC (1987) Nabis roseipennis adults (Hemiptera: Nabidae) as disseminators of nuclear polyhedrosis virus to Anticarsia gemmatalis (Lepidoptera: Noctuidae) larvae. Environ Entomol 16:1330–1333Google Scholar
  39. Young SY, Yearian WC (1992) Movement of nuclear polyhedrosis virus into velvetbean caterpillar (Lepidoptera: Noctuidae) larval populations on soybean by Nabis roseipellnis (Heteroptera: Nabidae) nymphs. J Entomol Sci 27:126–134Google Scholar
  40. Zhong Z, Xu Z, Ze-Peng (2011) Co-efficacy of a trap crop, Colocasia esculenta (L.) Schott and a biological agent, S. litura nuclear polyhedral virus on the tobacco caterpillar, S. litura (Fabricius) in the tobacco field. Pak J Zool 43(4):689–699Google Scholar

Copyright information

© International Organization for Biological Control (IOBC) 2013

Authors and Affiliations

  • R. K. Gupta
    • 1
  • Mudasir Gani
    • 1
    Email author
  • P. Jasrotia
    • 1
    • 2
  • K. Srivastava
    • 1
  1. 1.Division of EntomologySher-e-Kashmir University of Agricultural Sciences and Technology-JammuJammuIndia
  2. 2.Crop Protection Unit, Directorate of Groundnut Research ICARJunagadhIndia

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