, Volume 58, Issue 4, pp 553–561 | Cite as

The release and unsuccessful establishment of the Melaleuca biological control agent Fergusonina turneri and its mutualistic nematode Fergusobia quinquenerviae

  • Paul D. Pratt
  • Scott Blackwood
  • Susan A. Wright
  • Matthew Purcell
  • Min B. Rayamajhi
  • Robin M. Giblin-Davis
  • Sonja J. Scheffer
  • Philip W. Tipping
  • Ted D. Center


The Australian tree Melaleuca quinquenervia is an invasive weed in wetland systems of Florida, USA. A biological control program targeting M. quinquenervia resulted in the simultaneous release of the gall-fly Fergusonina turneri and the nematode Fergusobia quinquenerviae. Fergusonina (Diptera: Fergusoninidae) flies are gall formers that exploit plants in the Myrtaceae through a mutualistic association with nematodes in the genus Fergusobia (Tylenchida: Neotylenchidae). With a limited number of founding individuals, a risk-spreading release strategy was employed in 2005 by liberating a total of 1996 adult flies across seven locations in southern Florida. However, all release efforts failed to establish a viable population at any of the sites despite variation in location. In an effort to increase founding population size and improve phenological synchrony, 1,432 individual flies and associated nematodes were released within a single M. quinquenervia stand during the early winter months of 2006–2007. The population of F. turneri and F. quinquenerviae persisted at the field site for between two and three generations and, in accordance with the ca. 2-month generation time, emergence of F generation flies peaked in March, May and July 2007. Population growth rate increased with each succeeding generation up to the F3, after which the population went extinct. Both the F1 and F2 generations expanded spatially when compared to the distribution of their respective parental generations. The field population failed to spread after the F2 generation, with F3 generation galls found entirely within the spatial distribution of F2 galls. The release of F. turneri and F. quinquenerviae represent the first obligate mutualism used in weed biological control. Factors contributing to the failure of these species to establish are discussed.


Weed biological control Post release evaluation Spread Melaleuca quinquenervia 


  1. Andow DA, Kareiva PM, Levin SA, Okubo A (1993) Spread of invading organisms: patterns of spread. In: Kim KC, McPheron BA (eds) Evolution of insect pests: patterns of variation. John Wiley, New York, USA, pp 219–242Google Scholar
  2. Balciunas JK, Burrows DW, Purcell MF (1994) Field and laboratory host ranges of the Australian weevil, Oxyops vitiosa, a potential biological control agent of the paperbark tree, Melaleuca quinquenervia. Biol Control 4:351–360CrossRefGoogle Scholar
  3. Barton J, Fowler SV, Gianotti AF, Winks CJ, de Beurs M, Arnold GC, Forrester G (2007) Successful biological control of mist flower (Ageratina riparia) in New Zealand: agent establishment, impact and benefits to the native flora. Biol Control 40:370–385CrossRefGoogle Scholar
  4. Beirne BP (1975) Biological control attempts by introductions against pest insects in the field in Canada. Can Entomol 107:225–236CrossRefGoogle Scholar
  5. Boland DJ, Brooker MIH, Chippendale GM, Hall N, Hyland BPM, Johnston RD, Kleinig DA, Turner JD (1987) Forest trees of Australia. Nelson Wadsworth, Melbourne, AustraliaGoogle Scholar
  6. Butcher PA, Skinner AK, Gardiner CA (2005) Increased inbreeding and inter-species gene flow in remnant populations of the rare Eucalyptus benthamii. Conserv Genet 6:213–226CrossRefGoogle Scholar
  7. Center TD, Van TK, Rayachhetry MB, Buckingham GR, Dray FA, Wineriter SA, Purcell MF, Pratt PD (2000) Field colonization of the melaleuca snout beetle (Oxyops vitiosa) in south Florida. Biol Control 19:112–123CrossRefGoogle Scholar
  8. Center TD, Purcell MF, Pratt PD, Rayachhetry MB, Tipping PT, Wineriter S, Dray FA Jr (2012) Biological control of Melaleuca quinquenervia—an Everglades invader. BioControl 57:151–165CrossRefGoogle Scholar
  9. Cook LG, Morris DC, Edwards RD, Crisp MD (2008) Reticulate evolution in the natural range of the invasive wetland tree species Melaleuca quinquenervia. Mol Phylogenet Evol 47:506–522PubMedCrossRefGoogle Scholar
  10. Currie GA (1937) Galls on Eucalyptus trees. A new type of association between flies and nematodes. Proc Linn Soc NSW 62:147–176Google Scholar
  11. Davies KA, Giblin-Davis RM (2004) The biology and associations of Fergusobia (Nematoda) from the Melaleuca leucadendra-complex in eastern Australia. Invertebr Syst 18:291–319CrossRefGoogle Scholar
  12. Davies KA, Ye W, Giblin-Davis RM, Taylor GS, Scheffer SJ, Thomas WK (2010) The nematode genus Fergusobia (Nematoda: Neotylenchidae): molecular phylogeny, descriptions of clades and associated galls, host plants and Fergusonina fly larvae. Zootaxa 2633:1–66Google Scholar
  13. Dray FA Jr, Center TD, Wheeler GS (2001) Lessons from unsuccessful attempts to establish Spodoptera pectinicornis (Lepidoptera: Noctuidae), a biological control agent of waterlettuce. Biocontrol Sci Tech 11:301–316CrossRefGoogle Scholar
  14. Dray FA, Bennett BC, Center TD (2006) Invasion history of Melaleuca quinquenervia (Cav.) S.T. Blake in Florida. Castanea 71:210–225CrossRefGoogle Scholar
  15. Frankel OH, Soule ME (1981) Conservation and evolution. Cambridge University Press, Cambridge, UKGoogle Scholar
  16. Giblin-Davis RM, Davies KA, Williams DS, Center TD (2001a) Cuticular changes in fergusobiid nematodes associated with parasitism of fergusoninid flies. Comp Parasitol 68:242–248Google Scholar
  17. Giblin-Davis RM, Makinson J, Center BJ, Davies KA, Purcell M, Taylor GS, Scheffer SJ, Goolsby J, Center TD (2001b) Fergusobia/Fergusonina-induced shoot bud gall development on Melaleuca quinquenervia. J Nematol 33:239–247PubMedGoogle Scholar
  18. Giblin-Davis RM, Center BJ, Davies KA, Purcell MF, Scheffer SJ, Taylor GS, Goolsby J, Center TD (2004) Histological comparisons of Fergusobia/Fergusonina-induced galls on different Myrtaceous hosts. J Nematol 36:249–262PubMedGoogle Scholar
  19. Goeden RD, Louda SM (1976) Biotic interference with insects imported for weed control. Ann Rev Entomol 21:325–342CrossRefGoogle Scholar
  20. Goolsby JA, Makinson J, Purcell M (2000) Seasonal phenology of the gall-making fly Fergusonina sp. (Diptera: Fergusoninidae) and its implications for biological control of Melaleuca quinquenervia. Aust J Entomol 39:336–343CrossRefGoogle Scholar
  21. Grevstad FS (1999) Experimental invasions using biological control introductions: the influence of release size on the chance of population establishment. Biol Invasions 1:313–323CrossRefGoogle Scholar
  22. Jackson HD, Steane DA, Potts BM, Vaillancourt RE (1999) Chloroplast DNA evidence for reticulate evolution in Eucalyptus (Myrtaceae). Mol Ecol 8:739–751CrossRefGoogle Scholar
  23. McKinnon GE, Vaillancourt RE, Steane DA, Potts BM (2004) The rare silver gum, Eucalyptus cordata, is leaving its trace in the organellar gene pool of Eucalyptus globulus. Mol Ecol 13:3751–3762PubMedCrossRefGoogle Scholar
  24. McKinnon GE, Smith JJ, Potts BM (2010) Recurrent nuclear DNA introgression accompanies chloroplast DNA exchange between two eucalypt species. Mol Ecol 19:1367–1380PubMedCrossRefGoogle Scholar
  25. Pratt PD, Rayamajhi MB, Van TK, Center TD (2004) Modeling the influence of resource availability on population densities of Oxyops vitiosa, a biological control agent of the invasive tree Melaleuca quinquenervia. Biocontrol Sci Tech 14:51–61CrossRefGoogle Scholar
  26. Rayamajhi MB, Van TK, Pratt PD, Center TD (2006) Temporal and structural effects of stands on litter production in Melaleuca quinquenervia dominated wetlands of south Florida. Wetl Ecol Manag 14:303–316CrossRefGoogle Scholar
  27. Scheffer SJ, Giblin-Davis RM, Taylor GS, Davies KA, Purcell M, Lewis ML, Goolsby J, Center TD (2004) Phylogenetic relationships, species limits and host specificity of gall-forming Fergusonina flies (Diptera: Fergusoninidae) feeding on Melaleuca (Myrtaceae). Ann Entomol Soc Am 97:1216–1221CrossRefGoogle Scholar
  28. Silvers CS, Pratt PD, Ferriter AP, Center TD (2007) T.A.M.E. Melaleuca: a regional approach for suppressing one of Florida’s worst weeds. J Aquat Plant Manag 45:1–8Google Scholar
  29. Silvers CS, Pratt PD, Rayamajhi MB, Center TD (2008) Shoot demographics for melaleuca and impacts of simulated herbivory on vegetative development. J Aquat Plant Manag 46:121–125Google Scholar
  30. Story JM, Anderson NL (1978) Release and establishment of Urophora affinis (Diptera: Tephritidae) on spotted knapweed in western Montana. Environ Entomol 7:445–448Google Scholar
  31. Taylor GS (2004) Revision of Fergusonina Malloch gall flies (Diptera: Fergusoninidae) from Melaleuca (Myrtaceae). Invertebr Syst 18:251–290Google Scholar
  32. Taylor GS, Head ER, Davies KA (2004) Gall flies (Diptera: Fergusoninidae) on Myrtaceae: a mutualistic association between flies and nematodes. In: Raman A, Schaefer CW, Withers TM (eds) Biology, ecology and evolution of Gall-inducing arthropods, vol 2. Sci. Publ. Inc., Enfield, USA, pp 641–669Google Scholar
  33. Ye W, Giblin-Davis RM, Davies KA, Purcell M, Scheffer SJ, Taylor GS, Center TD, Morris K, Thomas WK (2007) Molecular phylogenetics and the evolution of host plant associations in the nematode genus Fergusobia (Tylenchida: Fergusobiinae). Mol Phylogenet Evol 45:123–141PubMedCrossRefGoogle Scholar

Copyright information

© International Organization for Biological Control (outside the USA) 2013

Authors and Affiliations

  • Paul D. Pratt
    • 1
  • Scott Blackwood
    • 2
  • Susan A. Wright
    • 3
  • Matthew Purcell
    • 4
  • Min B. Rayamajhi
    • 1
  • Robin M. Giblin-Davis
    • 5
  • Sonja J. Scheffer
    • 6
  • Philip W. Tipping
    • 1
  • Ted D. Center
    • 7
  1. 1.USDA-ARS, Invasive Plant Research LaboratoryFort LauderdaleUSA
  2. 2.USDA-APHIS-PPQDes PlainesUSA
  3. 3.USDA-ARS, Invasive Plant Research LaboratoryGainesvilleUSA
  4. 4.USDA-ARS, Australian Biological Control Laboratory, CSIRO Ecosystem SciencesBrisbaneAustralia
  5. 5.University of Florida, Institute of Food and Agricultural Services, Fort Lauderdale Research and Education CenterFort LauderdaleUSA
  6. 6.USDA-ARS, Systematic Entomology LaboratoryBeltsvilleUSA
  7. 7.USDA-ARS, Invasive Plant Research LaboratoryFort LauderdaleUSA

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