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Differential induction of grapevine resistance and defense reactions against Botrytis cinerea by bacterial mixtures in vineyards

Abstract

Previous experiments showed that seven bacteria identified as Acinetobacter lwoffii (PTA-113 and PTA-152), Bacillus subtilis (PTA-271), Pantoea agglomerans (PTA-AF1 and PTA-AF2) and Pseudomonas fluorescens (PTA-268 and PTA-CT2) induced systemic resistance in grapevine against Botrytis cinerea. Based on these findings, we investigated biocontrol capacity of different mixtures under vineyard conditions over three consecutive years. Treatments with bacterial mixtures were shown to induce systemic resistance against B. cinerea on year 2. Efficacy and duration of such a disease control seemed to be reinforced on year three without renewal of bacterial treatments. Accordingly, the effectiveness of induced resistance varied with mixture type of bacteria and was accompanied by a stimulation of chitinase and β-1,3 glucanase activities in both leaves and berries. Interestingly, treatments with mixtures containing both selected strains of P. agglomerans (PTA-AF1 + PTA-AF2) appeared as the most effective in triggering systemically the plant defense reactions and reducing the symptoms of grey mould disease.

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Acknowledgments

We thank the GDV members for their help with disease evaluation in vineyards. We also thank Dr. Amor Keziou for his kind help with the statistical analysis and Pr. Laurent Debelle for advices. We also gratefully acknowledge financial support from the Champagne Ardenne Regional Council (“Région Champagne Ardenne”, France) through Europol’Agro (program VINEAL, Reims, France).

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Correspondence to P. Trotel-Aziz.

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Handling Editor: Monica Höfte

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Magnin-Robert, M., Quantinet, D., Couderchet, M. et al. Differential induction of grapevine resistance and defense reactions against Botrytis cinerea by bacterial mixtures in vineyards. BioControl 58, 117–131 (2013). https://doi.org/10.1007/s10526-012-9474-y

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Keywords

  • Biocontrol
  • Grey mould
  • Induced systemic resistance
  • Bacterial mixtures
  • Vitis vinifera L.