Abstract
Pythium ultimum (Trow) is one of the main causes of damping-off disease in many parts of the world. Control of the disease depends mainly on application of chemical fungicides. However, soil treatments with fungicides are not always feasible due to economical and ecological reasons. Soil-borne, non-pathogenic bacteria of the genus Pseudomonas fluorescens with the ability to antagonise fungal phytopathogens, represent a realistic alternative to chemical fungicides and show great promise with respect to protect plant roots from fungal-induced diseases. In an attempt to find an integrated control system of damping-off disease in tomato, fungicides including azoxystrobin, metalaxyl-M and pyraclostrobin were applied alone and in combination with P. fluorescens isolate CW2. The fungicides were tested in in vitro for their antagonistic potential against P. ultimum and for compatibility with CW2. It was found that the fungicides were fungitoxic to P. ultimum, but did not inhibit the growth of the P. fluorescens. The efficacy of the fungicides alone and in combination with CW2 was also tested in greenhouse experiments against damping-off disease on tomato. Two concentrations (5 and 10 μg ml−1) were applied. Damping-off incidence of tomato seedlings in Humosoil®:sand mixture infested with P. ultimum was reduced following seed treatment with the fungicides. However, the degree of control obtained varied significantly depending on the fungicide used. Combined seed treatment with P. fluorescens and the fungicides resulted in a significant improvement in disease control and improved plant growth as indicated by shoot and root dry weights. Metalaxyl-M treatment applied alone or in combination with P. fluorescens, significantly protected tomato seedlings against damping-off. Strobilurin fungicides stimulated plant growth compared to metalaxyl-M. Combined treatment of tomato seeds with strobilurin fungicides and CW2 showed a moderate to good disease control and an increase in shoot and root dry weights.
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References
Anand T, Chandrasekaran A, Kuttalam S, Raguchander T, Samiyappan R (2009) Management of cucumber (Cucumis sativus L.) mildews through azoxystrobin-tolerant Pseudomonas fluorescens. J Agric Sci Technol 11:211–226
Andersen JB, Koch B, Nielsen TH, Sorensen D, Hansen M, Nybroe O, Christophersen C, Sorensen J, Molinand S, Givskov M (2003) Surface motility in Pseudomonas sp. DSS73 is required for efficient biological containment of the root-pathogenic microfungi Rhizoctonia solani and Pythium ultimum. Microbiology 149:37–46
Arndt W, Kolle C, Buchenauer H (1998) Effectiveness of fluorescent pseudomonads on cucumber and tomato plants under practical conditions and preliminary studies on the mode of action of antagonists. J Plant Dis Prot 105:198–215
Bartlett DW, Clough JM, Godwin JR, Hall AA, Hamer M, Parr-Dobrzanski B (2002) The strobilurin fungicides. Pest Manag Sci 58:649–662
Buchenauer H (1998) Biological control of soil-borne disease by rhizobacteria. J Plant Dis Prot 105(4):329–348
Cohen Y, Coffey MD (1986) Systemic fungicides and the control of oomycetes. Annu Rev Phytopathol 24:311–338
Conway KE, Maness NE, Motes JE (1997) Integration of biological and chemical controls for Rhizoctonia aerial blight and root rot of rosemary. Plant Dis 81:795–798
Deacon JW, Berry LA (1993) Biocontrol of soil-borne plant pathogens: concepts and their application. Pestic Sci 37:417–426
Diedhion PM, Oerke EC, Dehne HW (2004) Effect of the strobilurin fungicides azoxystrobin and Kresoxim-methyl on arbuscular mycorrhiza. J Plant Dis Prot 3(6):545–556
Dunne C, Moënne-Loccoz Y, McCarthy J, Higgins P, Powell J, Dowling DN, O’Gara F (1998) Combining proteolytic and phloroglucinol-producing bacteria for improved biocontrol of Pythium-mediated damping-off of sugar beet. Plant Pathol 47:299–307
Ellis RJ, Timms-Wilson TM, Beringer JE, Rhodes D, Renwick A, Stevenson L, Bailey MJ (1999) Ecological basis for biocontrol of damping-off disease by Pseudomonas fluorescens 54/96. J Appl Microbiol 87:454–463
Fakhouri W, Kang Z, Buchenauer H (2001) Microscopic studies on the mode of action of fluorescent pseudomonads alone and in combination with acibenzolar-S-methyl effective against Fusarium oxysporum f. sp. lycopersici in tomato plants. J Plant Dis Prot 108(5):513–529
Hassan MAE, Buchenauer H (2008) Enhanced control of bacterial wilt of tomato by DL-3-aminobutyric acid and the fluorescent Pseudomonas isolate CW2. J Plant Dis Prot 115(5):199–207
Herms S, Seehaus K, Koehle H, Conrath U (2003) A strobilurin fungicide enhances the resistance of tobacco mosaic virus and Pseudomonas syringae pv tabaci. Plant Physiol 130:120–127
Hwang SF (1994) Potential for integrated biological and chemical control of seeding rot and preemergence damping off caused by Fusarium avenaceum in Lentil with Bacillus subtilis and Viraflo®-280. J Plant Dis Prot 101(2):188–199
King EO, Ward MK, Raney DE (1954) Two simple media for the demonstration of pyocynin and fluorescin. J Lab Clin Med 44:301–307
Lewis JA, Papavizas GC (1987a) Reduction of inoculum Rhizoctonia solani in soil by germlings of Trichoderma hamatum. Soil Biol Biochem 19:195–201
Lewis JA, Papavizas GC (1987b) Application of Trichoderma and Gliocladium in alginate pellets for control of Rhizoctonia damping-off. Plant Pathol 36:438–446
Lifshitz R, Libshitz S, Baker R (1985) Decrease in incidence of Rhizoctonia preemergence damping-off by the use of integrated chemical and biological controls. Plant Dis 69:431–434
Mao W, Lewis JA, Hebber PK, Lumsden RD (1997) Seed treatment with a fungal or a bacterial antagonist for reducing corn damping-off caused by species of Pythium and Fusarium. Plant Dis 81:450–454
Salman M (2010) Determination of antibiotic activity on plasmids from fluorescent Pseudomonads isolates CW2, WB15 and WB52 against pre-emergence damping-off caused by Pythium ultimum and Rhizoctonia solani in cucumber. Biol Control 53(2):161–167
Staub T, Dahmen H, Shwinn FJ (1978) Biological characterization of uptake and translocation of fungicidal acylalanines in grape and tomato plants. J Plant Dis Prot 87:162–168
Ulrike MS, Werner D (1998) Enhancement of the antagonistic potential of Pseudomonas fluorescens towards Pythium ultimum on cucumber (Cucumis sativus L.) by flavonoids. J Plant Dis Prot 105(2):157–167
Vogt W, Buchenauer H (1997) Enhancement of biological control by combination of antagonistic fluorescent pseudomonas isolates and resistance inducers against damping-off and powdery mildew in cucumber. J Plant Dis Prot 104(3):272–280
Wang SF, Chakravarty P, Prevost D (1993) Effects of rhizobia, metalaxyl, and VA mycorrhizal fungi on growth, nitrogen fixation, and development of Pythium root rot of Sainfoin. Plant Dis 77:1093–1098
Weller DM (1988) Biological control of soil-borne plant pathogens in the rhizosphere with bacteria. Annu Rev Phytopathol 26:379–407
Whipps JM (2001) Microbial interactions and biocontrol in the rhizosphere. J Exp Bot 52:487–511
Acknowledgments
The financial support of the German Academic Exchange services (DAAD) is highly acknowledged and appreciated. The author deeply appreciate the efforts of Prof. H. Buchenauer, Institute of Phytomedicine (360), University of Hohenheim, Otto-Sander-Str. 5, D-70599 Stuttgart, Germany for providing supervision on the work.
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Salman, M., Abuamsha, R. Potential for integrated biological and chemical control of damping-off disease caused by Pythium ultimum in tomato. BioControl 57, 711–718 (2012). https://doi.org/10.1007/s10526-012-9444-4
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DOI: https://doi.org/10.1007/s10526-012-9444-4