Abstract
Aeromonas hydrophila SBK1 (GenBank accession no. HM802878.1), a potent chitinolytic bacterium, was isolated from a pool of 30 chitinolytic isolates. The isolate showed higher chitinolytic activity in respect to clear zone to colony size ratio of 2.15. Maximum production of chitinolytic enzymes, viz., β-N-acetyl-glucosaminidase and chitinase (specific activity 655.3 and 71.6 U mg−1, respectively) by A. hydrophila SBK1 was observed in the synthetic media, containing (w/v)-colloidal chitin, 4.0%; peptone, 0.3%; phosphate, 0.3% (0.15% of each KH2PO4 and K2HPO4); NaCl, 0.25%; MgSO4, 0.05%; KCl, 0.05%; pH 7.0 and at 35°C after 72 h of incubation. Both carbon-to-nitrogen (C/N) and carbon-to-phosphate (C/P) ratio of 13.33 were found optimum for chitinase production. Enzyme productivity increased about twofold in optimized culture condition in respect to its un-optimized state. The crude enzyme showed optimum activity against Culex quinquefasciatus larvae in native water at pH 7.0 and 35°C (LD50 0.60 U ml−1 at 48 h). Therefore, the studied chitinases can be used as an effective mosquitocidal agent.
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The work was supported by the financial support of the Major Research Project sponsored by University Grants Commission (UGC), New Delhi, India [F.NO.-33-218/2007(SR)].
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Halder, S.K., Maity, C., Jana, A. et al. Chitinolytic enzymes from the newly isolated Aeromonas hydrophila SBK1: study of the mosquitocidal activity. BioControl 57, 441–449 (2012). https://doi.org/10.1007/s10526-011-9405-3
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DOI: https://doi.org/10.1007/s10526-011-9405-3