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Iranian Trichogramma: ITS2 DNA characterization and natural Wolbachia infection

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Abstract

Egg parasitoids of the genus Trichogramma (Hymenoptera: Trichogrammatidae) are important natural enemies of many lepidopterous pests in agricultural and forest areas. Because the use of indigenous Trichogramma populations/species can significantly increase the biological control efficiency, the characterization of endemic populations and precise species identification is important. In this study, Trichogramma wasps were collected from parasitized eggs of several lepidopteran pests in Northern and Central Iran. Seven Trichogramma species were identified based on the morphology and the nucleotide sequence, size and restriction profile (PCR-RFLP) of the internal transcribed spacer 2 (ITS2) region of the rDNA of T. brassicae Bezdenko, T. cacoeciae Marchal, T. embryophagum Hartig, T. evanescens Westwood, T. euproctidis Girault, T. pintoi Voegelé and T. tshumakovae Sorokina. This is the first report of T. euproctidis in Iran. Additionally, natural populations were surveyed for the prevalence of Wolbachia. Two populations of T. brassicae were found to be infected by a single Wolbachia strain which was identified by using a multi-locus sequencing approach.

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Acknowledgments

We are grateful to Dr. Bernard Pintureau in (INRA/INSA) France for morphological identifying Trichogramma samples. Thijs Machtelinckx is supported by grant number SB-73469 from the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen). Thomas Van Leeuwen is a post-doctoral fellow of the Research Foundation Flanders (FWO).

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  1. Department of Plant Protection, University of Tehran, P.O. Box 4111, Karaj, Iran

    Nafiseh Poorjavad & Seyed H. Goldansaz

  2. Laboratory of Agrozoology, Department of Crop Protection, Ghent University, Coupure Links 653, 9000, Ghent, Belgium

    Thijs Machtelinckx, Luc Tirry & Thomas van Leeuwen

  3. Department of Entomology, University of California, Riverside, CA, 92521, USA

    Richard Stouthamer

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  1. Nafiseh Poorjavad
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  2. Seyed H. Goldansaz
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Correspondence to Seyed H. Goldansaz.

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Poorjavad, N., Goldansaz, S.H., Machtelinckx, T. et al. Iranian Trichogramma: ITS2 DNA characterization and natural Wolbachia infection. BioControl 57, 361–374 (2012). https://doi.org/10.1007/s10526-011-9397-z

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