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Molecular characterization of Psyttalia lounsburyi, a candidate biocontrol agent of the olive fruit fly, and its Wolbachia symbionts as a pre-requisite for future intraspecific hybridization

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Abstract

Numerous arthropod species are genetically differentiated across their distribution area. Diversifying the geographical origins of a biocontrol agent species can be used to favour their perennial establishment by the sampling of pre-adapted genotypes and/or the production of new genotypes through hybridization. Hybridization can be nevertheless challenged by reproductive isolations induced by some common microbial endosymbionts. In this study, we aimed at characterizing (i) the genetic diversity of six populations of Psyttalia lounsburyi (Hymenoptera: Braconidae), a candidate biocontrol agent of the olive fruit fly Bactrocera oleae (Diptera: Tephritidae) and (ii) the diversity of their Wolbachia endosymbionts. Both mitochondrial and microsatellite markers evidence clustering between the South African population and several Kenyan/Namibian populations. The survey of the Wolbachia also distinguished two main variants with a spatial heterogeneity in the infection status. All these results are discussed in the context of the use of these P. lounsburyi populations for hybridization and further field releases.

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Authors and Affiliations

  1. UMR INRA-CNRS-UNSA 1301 IBSV (Interactions Biotiques et Santé Végétale), 400 Route des Chappes, BP 167, 06903, Sophia Antipolis cedex, France

    Sandrine Cheyppe-Buchmann, Thibaut Malausa & Xavier Fauvergue

  2. European Biological Control Laboratory, ARS–USDA Campus International de Baillarguet, CS 90013, 34988, Saint Gely-du-Fesc cedex, France

    Marie-Claude Bon & Walker Jones

  3. UE INRA 1254 Lutte Biologique, 400 Route des Chappes, BP 167, 06903, Sophia Antipolis cedex, France

    Sandrine Cheyppe-Buchmann, Sylvie Warot & Nicolas Ris

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  1. Sandrine Cheyppe-Buchmann
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  2. Marie-Claude Bon
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  3. Sylvie Warot
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  4. Walker Jones
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  5. Thibaut Malausa
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  6. Xavier Fauvergue
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  7. Nicolas Ris
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Correspondence to Nicolas Ris.

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Cheyppe-Buchmann, S., Bon, MC., Warot, S. et al. Molecular characterization of Psyttalia lounsburyi, a candidate biocontrol agent of the olive fruit fly, and its Wolbachia symbionts as a pre-requisite for future intraspecific hybridization. BioControl 56, 713–724 (2011). https://doi.org/10.1007/s10526-011-9346-x

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