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Lethal and sublethal effects of Iranian isolates of Steinernema feltiae and Heterorhabditis bacteriophora on the Colorado potato beetle, Leptinotarsa decemlineata

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Abstract

To determine the LC50 values of two entomopathogenic nematodes against Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae) prepupae, different concentrations of the nematodes were tested in soil. Because of the different temperature requirements of the two nematode species, bioassay experiments were conducted at 20 ± 1°C and 27 ± 2°C for Steinernema feltiae Filipjev (Rhabditida: Steinernematidae) and Heterorhabditis bacteriophora Poinar (Rhabditida: Heterorhabditidae), respectively. Both the isolates were effective against L. decemlineata. LC50 values of H. bacteriophora against progeny of field-collected adults and laboratory-reared adults were estimated as 8.5 and 7.6 IJ per prepupa, respectively. For S. feltiae the value was calculated as 51.2 IJ per prepupa against offspring of laboratory-reared adults of L. decemlineata only. Cellular encapsulation of both nematode species was observed. Sublethal nematode concentrations caused wing deformation and delayed metamorphosis which may affect Colorado potato beetle adult fitness.

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Authors and Affiliations

  1. Nematology Laboratory, Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

    L. Ebrahimi & G. Niknam

  2. Department of Nematology, University of California, Davis, USA

    E. E. Lewis

Authors
  1. L. Ebrahimi
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  2. G. Niknam
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  3. E. E. Lewis
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Correspondence to G. Niknam.

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Handling Editor: Ralf Ehlers.

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Ebrahimi, L., Niknam, G. & Lewis, E.E. Lethal and sublethal effects of Iranian isolates of Steinernema feltiae and Heterorhabditis bacteriophora on the Colorado potato beetle, Leptinotarsa decemlineata . BioControl 56, 781–788 (2011). https://doi.org/10.1007/s10526-011-9343-0

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  • DOI: https://doi.org/10.1007/s10526-011-9343-0

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