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Individual and combined effects of Bacillus thuringiensis var. israelensis, temephos and Leptolegnia chapmanii on the larval mortality of Aedes aegypti

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Abstract

Larvicidal effects of interaction between Bacillus thuringiensis var. israelensis (Bti), temephos and Leptolegnia chapmanii zoospores on larvae of Aedes aegypti were determined under laboratory and seminatural conditions. In laboratory bioassays, two concentrations of Bti (0.012, 0.027 ppm), two of temephos (0.00035, 0.001 ppm), and a single concentration of L. chapmanii zoospores (6.1 × 104 zoospores ml−1) were evaluated. Trials under field-like conditions were performed in a single container and then placed either in the shade or in direct exposure to sunlight. We evaluated concentrations of Bti and temephos at 3-fold those normally used in laboratory tests: 0.09 and 0.003 ppm, respectively, plus 1.8 × 105 zoospores ml−1 of L. chapmanii. The combined effect of sublethal concentrations of Bti, temephos, and L. chapmanii zoospores thus indicated that this fungus is not inhibited by the larvicides and also demonstrated the synergistic effect of the action of L. chapmanii when used together with Bti and temephos.

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Notes

  1. We acknowledge that all taxa of oomycete water molds are now classified among the Kingdom Chromista (=Straminipila) and formally excluded from the true fungi. For the sake of convenience, however, we continue to refer to oomycetes in this paper in the historically sense as fungi.

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Acknowledgments

This study was partially supported by Agencia National de Promoción Científica y Técnica (PICT No 11118/02), CONICET, and Universidad Nacional de La Plata, La Plata, Argentina. We thank Dr. Joop Van Lenteren for critical reading of the manuscript. The authors also thank to Dr. Donald F. Haggerty, a retired career investigator and native English speaker, for editing the final version of the manuscript.

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Authors and Affiliations

  1. CEPAVE (Centro de Estudios Parasitológicos y de Vectores) CCT La Plata-Conicet-UNLP, Calle 2 # 584, 1900, La Plata, Argentina

    Sebastian A. Pelizza, Claudia C. López Lastra & Juan J. García

  2. Instituto de Botánica Carlos Spegazzini. Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Argentina, 53 # 477, 1900, La Plata, Argentina

    Ana C. Scorsetti

  3. Cátedra de Estadística, Facultad de Agronomía, Universidad Nacional de Rosario, Rosario, Argentina

    Vilma Bisaro

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  1. Sebastian A. Pelizza
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  2. Ana C. Scorsetti
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  3. Vilma Bisaro
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  4. Claudia C. López Lastra
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  5. Juan J. García
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Correspondence to Sebastian A. Pelizza.

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Handling Editor: Martin Erlandson.

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Pelizza, S.A., Scorsetti, A.C., Bisaro, V. et al. Individual and combined effects of Bacillus thuringiensis var. israelensis, temephos and Leptolegnia chapmanii on the larval mortality of Aedes aegypti . BioControl 55, 647–656 (2010). https://doi.org/10.1007/s10526-010-9281-2

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