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Interactions of Colletotrichum musae and Lasiodiplodia theobromae and their biocontrol by Pantoea agglomerans and Flavobacterium sp. in expression of crown rot of “Embul” banana

Abstract

Previous research has shown that two local isolates of bacteria (Pantoea agglomerans and Flavobacterium sp.) are capable of biocontrol of the two main pathogens (Colletotrichum musae and Lasiodiplodia theobromae) known to cause crown rot on “Embul” (Musa, AAB) banana. In this investigation an attempt was made to elucidate the comparative virulence of these pathogens and to determine the underlying biocontrol mechanisms. L. theobromae was more virulent, causing faster spread of the disease, whereas C. musae was more resistant to the bacterial antagonists. Viable cells of the antagonists were more effective at suppressing conidial germination than cell-free culture media. It seemed that antifungal compounds acting on conidial germination may be heat stable and those acting on mycelia may include heat labile compounds also. Considering the specialized roles observed for each pathogen in terms of causing the disease, and in modes of control by antagonists, future field investigations on biocontrol should consider the roles played by the pathogens and the antagonists.

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Acknowledgements

We are much indebted to Professor N.K.B. Adikaram for discussions of fungal pathogens of banana. Financial assistance by the National Science Foundation, Sri Lanka, research Grant No. RG/99/B/6 and Hilda Obeysekera Research fellowship, are acknowledged.

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Correspondence to Anjani M. Karunaratne.

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Handling Editor: Monica Hofte.

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Niroshini Gunasinghe, W.K.R., Karunaratne, A.M. Interactions of Colletotrichum musae and Lasiodiplodia theobromae and their biocontrol by Pantoea agglomerans and Flavobacterium sp. in expression of crown rot of “Embul” banana. BioControl 54, 587–596 (2009). https://doi.org/10.1007/s10526-009-9210-4

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Keywords

  • Antibiotic compounds
  • Antagonism
  • Mode of action