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Virulence of entomopathogenic hypocrealean fungi infecting Anoplophora glabripennis

An Erratum to this article was published on 17 October 2007


Twenty isolates of four species of entomopathogenic hypocrealean fungi (Beauveria bassiana, Beauveria brongniartii, Isaria farinosa, and Metarhizium anisopliae) were found to be pathogenic to adults of the Asian longhorned beetle, Anoplophora glabripennis. Survival times for 50% of the beetles tested (ST50) ranged from 5.0 (M. anisopliae ARSEF 7234 and B. brongniartii ARSEF 6827) to 24.5 (I. farinosa ARSEF 8411) days. Screening studies initially included strains of B. brongniartii, which is registered as a microbial control agent in Europe, Asia and South America but not in North America. At that time, we could not confirm that this fungal species is native to North America which added uncertainty regarding future registration of this species for pest control in the USA. Therefore, subsequent bioassays documented median survival times for three M. anisopliae isolates (5–6 days to death) and two of these isolates are suggested for further development because they are already registered for pest control in the USA.

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  • Animal Plant Health Inspection Service [APHIS] (2006) The Asian longhorned beetle cooperative eradication program. ALB Newsletter 1, 8 pp. [accessed 25 May 2007]

  • Animal Plant Health Inspection Service [APHIS] (2007) Asian longhorned beetle. [accessed 25 May 2007]

  • Canadian Forest Service [CFS] (2007) Asian longhorned beetle. [accessed 25 May 2007]

  • David L (1993) Microsporidian infection of the alfalfa snout beetle, Otiorrhynchus ligustici (Coleoptera: Curculionidae), and biological protection of hop plants. Eur J Entomol 90:99–100

    Google Scholar 

  • Dubois TLM (2003) Biological control of the Asian longhorned beetle, Anoplophora glabripennis, with entomopathogenic fungi. Ph.D. Dissertation, Cornell University, Ithaca, New York, 200 pp

  • Dubois T, Hajek AE, Smith S (2002) Methods for rearing the Asian longhorned beetle (Coleoptera: Cerambycidae) on artificial diet. Ann Entomol Soc Am 95:223–230

    Article  Google Scholar 

  • Dubois T, Hajek AE, Jiafu H, Li Z (2004a) Evaluating the efficiency of entomopathogenic fungi against the Asian longhorned beetle, Anoplophora glabripennis (Coleoptera: Cerambycidae), using cages in the field. Environ Entomol 33:62–74

    Google Scholar 

  • Dubois T, Li Z, Jiafu H, Hajek AE (2004b) Efficacy of fiber bands impregnated with Beauveria brongniartii cultures against Asian longhorned beetle, Anoplophora glabripennis (Coleoptera: Cerambycidae). Biol Control 31:320–328

    Article  Google Scholar 

  • Faria MR de, Wraight SP (2007) Mycoinsecticides and mycoacaricides: a comprehensive list with worldwide coverage and international classification of formulation types. Biol Control (in press)

  • Gasman B (2006) Update on the Asian long-horned beetle (Anoplophora glabripennis)—Canada. North American Plant Protection Organization’s Phytosanitary Alert System: Official Pest Reports.

  • Goettel MS, Inglis GD (1997) Fungi: Hyphomycetes. In: Lacey LA (ed) Manual of techniques in insect pathology. Academic Press, San Diego, CA, pp 213–250

    Google Scholar 

  • Haack RA, Bauer LS, Poland TM, Petrice TR, Miller DL, Liu H (2003) 2002 studies on the Asian longhorned beetle, pine shoot beetle, and emerald ash borer. USDA, For Serv Gen Tech Rpt NE-315:27

    Google Scholar 

  • Hajek AE (2007) Asian longhorned beetle: ecology and control. In: Pimentel D (ed) Encyclopedia of Pest Management, vol II. CRC Press, Boca Raton, FL, pp 21–24

  • Hajek AE, Curtiss RT, Liebherr JK (2004) Characters differentiating male from female Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae). Proc Entomol Soc Wash 106:928–931

    Google Scholar 

  • Hajek AE, Huang B, Dubois T, Smith MT, Li Z (2006) Field studies of control of Anoplophora glabripennis (Coleoptera: Cerambycidae) using fiber bands containing the entomopathogenic fungi Metarhizium anisopliae and Beauveria brongniartii. Biocontr Sci Technol 16:329–343

    Article  Google Scholar 

  • Hérard F, Krehan H, Benker U, Boegel C, Schrage R, Chauvat E, Ciampitti M, Maspero M, Bialooki P (2005) Anoplophora in Europe: infestations and management responses. USDA, For Serv Gen Tech Rept NE-337:35–40

    Google Scholar 

  • Higuchi T (1999) Biolisa® Kamakiri—a biorational agricultural pesticide contributing to environmental conservation. Nitto Tech Rep 37:49–51

    Google Scholar 

  • Higuchi T, Saika T, Senda S, Mizobata T, Kawata Y, Nagai J (1997) Development of biorational pest control formulation against longicorn beetles using a fungus, Beauveria brongniartii (Sacc.) Petch. J Ferment Bioengin 84:236–243

    Article  CAS  Google Scholar 

  • Keena MA (2005) Pourable artificial diet for rearing Anoplophora glabripennis (Coleoptera: Cerambycidae) and methods to optimize larval survival and synchronize development. Ann Entomol Soc Am 98:536–547

    Article  CAS  Google Scholar 

  • Keena MA (2006) Effects of temperature on Anoplophora glabripennis (Coleoptera: Cerambycidae) adult survival, reproduction, and egg hatch. Environ Entomol 35:912–921

    Google Scholar 

  • Khachatourians GG (1992) Virulence of five Beauveria strains, Paecilomyces farinosus, and Verticillium lecanii against the migratory grasshopper, Melanoplus sanguinipes. J Invertebr Pathol 59:212–214

    Article  Google Scholar 

  • Kish LP, Terry I, Allen GE (1977) Three fungi tested against the lovebug, Plecia nearctica, in Florida. Fl Entomol 60:291–295

    Article  Google Scholar 

  • Lingafelter SW, Hoebeke ER (2002) Revision of Anoplophora (Coleoptera: Cerambycidae). Entomol. Soc. Wash., Wash., DC, 236 pp

  • Linsley EG (1959) Ecology of Cerambycidae. Annu Rev Entomol 4:99–138

    Article  Google Scholar 

  • Liu R, Yamazaki M (1996) Progress of the cooperative study “Ningxia Forest Protection Study Project” between Japan and China. Ningxia For Prot Res 2:26–29

    Google Scholar 

  • Nowak DJ, Pasek JE, Sequeira RA, Crane DE, Mastro VC (2001) Potential effect of Anoplophora glabripennis (Coleoptera: Cerambycidae) on urban trees in the United States. J Econ Entomol 94:116–122

    PubMed  CAS  Google Scholar 

  • Pinnock DE, Garcia R, Cubbin CM (1973) Beauveria tenella as a control agent for mosquito larvae. J Invertebr Pathol 22:143–147

    Article  PubMed  CAS  Google Scholar 

  • Poland TM, Haack RA, Bauer LS, Petrice TR, Miller DL, Liu H-P (2002) Overview of Asian longhorned beetle research by the USDA Forest Service, North Central Research Station, East Lansing, Michigan. USDA, For Serv Gen Tech Rept NE-300:75

    Google Scholar 

  • Poland TM, Haack RA, Petrice TR, Miller DL, Bauer LS, Gao R-T (2006a) Field evaluations of systemic insecticides for control of Anoplophora glabripennis (Coleoptera: Cerambycidae) in China. J Econ Entomol 99:383–392

    Article  PubMed  CAS  Google Scholar 

  • Poland TM, Haack RA, Petrice TR, Miller DL, Bauer LS (2006b) Laboratory evaluation of the toxicity of systemic insecticides for control of Anoplophora glabripennis and Plectrodera scalator (Coleoptera: Cerambycidae). J Econ Entomol 99:85–93

    Article  PubMed  Google Scholar 

  • Prest DB, Gilliam M, Taber S III, Mills JP (1974) Fungi associated with discolored honey bee, Apis mellifera, larvae and pupae. J Invertebr Pathol 24:253–255

    Article  PubMed  CAS  Google Scholar 

  • Rehner SA, Buckley E (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97:84–98

    Article  PubMed  CAS  Google Scholar 

  • Sanders RD (1972) Microbial mortality factors in Aedes sierrensis populations. Proceedings and papers of the 40th annual conference of the California Mosquito Control Association, Jan. 31–Feb. 2, 1972, pp 66–68

  • SAS Institute Inc (2006) SAS software release 9.1.3. SAS Institute Inc., Cary, North Carolina

    Google Scholar 

  • Shimane T, Kawakami K (1994) Pathogenicity of an entomogenous fungus Beauveria brongniartii (Saccardo) Petch against the yellow-spotted longicorn beetle Psacothea hilaris Pascoe and mycological characteristics of the fungus. Bull Natl Inst Seric Entomol Sci 10:1–36

    Google Scholar 

  • Shimazu M (1994) Potential of the cerambycid-parasitic type of Beauveria brongniartii (Deuteromycotina: Hyphomycetes) for microbial control of Monochamus alternatus Hope (Coleoptera: Cerambycidae). Appl Entomol Zool 29:127–130

    Google Scholar 

  • Shimazu M, Zhang B, Liu Y (2002) Fungal pathogens of Anoplophora glabripennis (Coleoptera: Cerambycidae) and their virulences. Bull For Prod Res Inst 1:123–130

    Google Scholar 

  • Smith MT, Tobin PC, Bancroft J, Li G, Gao R (2004) Dispersal and spatiotemporal dynamics of Asian longhorned beetle (Coleoptera: Cerambycidae) in China. Environ Entomol 33:435–442

    Article  Google Scholar 

  • Soares GG (1980) A study of Tolypocladium cylindrosporum Gams, a new, naturally occurring fungal pathogen of mosquitoes (with notes on Beauveria bassiana (Bals.) Vuill.). Ph.D. Dissertation, University of California, Berkeley, California

  • Wada S, Horita M, Hirayae K, Shimazu M (2003) Discrimination of Japanese isolates of Beauveria brongniartii (Deuteromycotina: Hyphomycetes) by RFLP of the rDNA-ITS regions. Appl Entomol Zool 38:551–557

    Article  CAS  Google Scholar 

  • Wang S, Shao Q, Shi Y, Zhao M (1990) Isolation and identification of Beauveria bassiana from the larvae of Anoplophora glabripennis (Coleoptera: Cerambycidae). Inner Mongolia For Sci Technol 1:14–16

    Google Scholar 

  • Wang S, Shi Y, Zhao M, Zou L (1999) Studies on the joint effects of Scleroderma guani (Hymenoptera: Bethylidae) and Beauveria bassiana and Paecilomyces farinosus in the control of Anoplophora glabripennis. Inner Mongolia For Sci Technol 1:39–42

    Google Scholar 

  • Wang S, Zhao M, Shi Y (1997) Isolation and identification of Paecilomyces farinosus from the larvae of Anoplophora glabripennis. For Pest Dis 4:26–27

    Google Scholar 

  • Zhang B, Liu Y, Bai Y, Shimazu M (1999) Pathogenic fungi of Anoplophora spp. (Coleoptera: Cerambycidae) in Ningxia Hui Autonomous Region and their virulence. J Beijing For Univ 21:67–72

    Google Scholar 

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We thank M. Wheeler for conducting some of the screening bioassays, J. Vandenberg for use of the USDA-ARS quarantine facility in Ithaca, NY and M. Smith, S. Teale, V. d’Amico, S. Smith, M. Shimazu, Nitto Denko, R. Humber, J. Lord, S. Jaronski and Z. Li for assistance with fungal isolates. R. Humber and L. Sigler helped with fungal identifications. Z. Li’s students, especially H. Jiafu and X. Zhang, were very kind in helping us obtain isolates in China. L. Li assisted with compiling information about B. brongniartii and L. Zhang translated critical Chinese literature. J. Losey, J. Vandenberg, and S. Wraight, assisted with analysis and interpretation of data, and F. Vermeylen assisted with advice about statistical tests. Studies were funded in part by Alphawood Foundation and a USDA-FS cooperative agreement.

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Correspondence to Ann E. Hajek.

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Dubois, T., Lund, J., Bauer, L.S. et al. Virulence of entomopathogenic hypocrealean fungi infecting Anoplophora glabripennis . BioControl 53, 517–528 (2008).

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  • Anoplophora glabripennis
  • Asian longhorned beetle
  • Beauveria bassiana
  • Beauveria brongniartii
  • Metarhizium anisopliae
  • Entomopathogenic fungi
  • Biological control
  • Bioassay