Abstract
A nucleopolyhedrovirus (MaviMNPV) was isolated from diseased larvae of legume pod borer (LPB), Maruca vitrata, at Tainan in Taiwan. Electron microscopical studies on the ultrastructure of MaviMNPV occlusion bodies (OBs) showed several virions (up to 19) with multiple nucleocapsids (up to 6) packaged within a single viral envelope. The diameter of OBs was 0.9 to 1.3 µm with a mean of 1.152±0.116 µm. The complete sequence of the MaviMNPV polyhedrin (Polh) gene contained 735 nucleotides (GenBank accession number DQ399596). Phylogenetic analyses using the complete sequence of the Polh gene of MaviMNPV indicated that this virus clusters with Group I NPVs. The genome size of MaviMNPV estimated with restriction enzymes viz., HindIII, EcoRI, BglII and PstI was 113.41 ± 1.50 kbp. First instar LPB larvae were the most susceptible stage (LC50 2.053 × 102 OBs/ml) followed by second, third and fourth instars with the median lethal concentrations (LC50s) 1.410 × 103, 2.390 × 103 and 2.636 × 103 OBs/ml, respectively. This is the first record of this virus from this region.
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Acknowledgement
This study was supported by the Bureau of Animal and Plant Health Inspection and Quarantine (BAPHIQ), Council of Agriculture, Republic of China under Grant No. COA94AS-13.2.1-BQ-B6. The authors acknowledge assistance rendered by Dr. Chung-Hsiung Wang, Department of Entomology, National Taiwan University, Taipei, Taiwan for transmission electron microscopy.
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Lee, ST., Srinivasan, R., Wu, YJ. et al. Occurrence and characterization of a nucleopolyhedrovirus from Maruca vitrata (Lepidoptera, Pyralidae) isolated in Taiwan. BioControl 52, 801–819 (2007). https://doi.org/10.1007/s10526-006-9049-x
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DOI: https://doi.org/10.1007/s10526-006-9049-x