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Augmentative biological control of whiteflies using transplants

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Abstract

Field studies showed that transplants can be used to move parasitoids into fields of commercially grown cantaloupe, Cucumis melo (Cucurbitaceae), and augment parasitism of sweet potato whitefly, Bemisia tabaci biotype B (= Bemisia argentifolii) (Homoptera: Aleyrodidae). Methods were developed to inoculate cantaloupe seedlings with newly imported Eretmocerus spp. (Hymenoptera: Aphelinidae), then transfer plants into both organic and conventional fields of cantaloupe in the desert growing region of southeastern California. Several obstacles to inoculating “banker plants” with an adequate number of parasitized whiteflies were overcome and numbers of parasitoids per transplant increased. In 1999 the use of banker plants was compared to a standard hand-release method and a no-release control in a replicated study at an organic farm. Augmentation through releases of parasitoids increased parasitism over that in the no-release controls (p <0.05). Banker plants increased the proportion of parasitized whiteflies more than the hand-release method (0.21 vs. 0.08). During a region-wide demonstration spring 2000, plots receiving banker plants significantly increased parasitism over paired control plots at seven commercial farms of cantaloupe. Parasitism in banker plant treated plots in 2000 was higher in organic fields (seasonal average =0.30) than conventional ones (seasonal average =0.06). Differences may be due to the use of imidacloprid, a systemic insecticide, in conventional fields for whitefly control. Over the 2-year study, however, releases of parasitoids did not consistently reduce densities of B. tabaci. Only in late season at some sites in 2000 were whitefly densities lower in release plots than paired controls. Most of the parasitoids recovered and identified from plots receiving parasitoids were the same as those released, Eretmocerus spp. (ex. Ethiopia M96076), and E. hayati(M95012, ex. Pakistan).

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References

  • Bellamy, D. E. and D. N. Byrne, 2001. Effects of gender and mating status on self-directed dispersal by the white fly parasitoid Eretmocerus eremicus. Ecol. Entomol. 26: 571–577.

    Google Scholar 

  • Brazzle, J. R., K. M. Heinz and M. P. Parrella, 1997. Multivariate approach to identifying patterns of Bemisia argentifolii (Homoptera:Aleyrodidae)infesting cotton. Environ. Entomol. 26:995–1003.

    Google Scholar 

  • Byrne, F. J., S. Castle, N. Prabhaker and N. C. Toscano, 2003. Biochemical study of resistance to imidacloprid in B biotype Bemisia tabaci from Guatemala. Pest Manage. Sci. 59:347–352.

    Google Scholar 

  • Byrne, F. J., I. Denholm, L. C. Birnie, A. L. Devonshire and M. W. Rowland, 1992. Analysis of insecticide resistance in the White fly, Bemisia tabaci. In:I. A. L. Devonshire Denholm and D. W. Hollomon (eds), Resistance '91:Achievements and Developments in Combating Pesticide Resistance. Elsevier, London. pp. 165–178.

    Google Scholar 

  • Byrne, D. N., R. J. Rathman, T. V. Orum and J. C. Palumbo, 1996. Localized migration and dispersal by the sweet potato white fly, Bemisia tabaci. Oecologia 105:320–328.

    Google Scholar 

  • Chu, C. C., T. J. Henneberry and A. C. Cohen, 1995. Bemisia argentifolii (Homoptera: Aleyrodidae):host preference and factors affecting oviposition and feeding site preference. Environ. Entomol. 24:354–360.

    Google Scholar 

  • Conte, L., F. Chiarini, A. Zancanaro and L. D. Monta`, 2000. Biological control of Aphis gossypii Glover (Rhynchota, Aphididae)in organic greenhouse cucumbers using “banker plants” as open rearing units of beneficials:two years of trials. In:Proceedings 13th International IFOAM Scientific Conference, Basel, Switzerland, 28–31 August, 2000.

  • DeBach, P. (ed. ), 1973. Biological Control of Insect Pests and Weeds. Chapman and Hall London. 844 pp.

    Google Scholar 

  • DeBach, P. and D. Rosen, 1991. Biological Control by Natural Enemies. Cambridge University Press. 440 pp.

  • Ellsworth, P. C. and J. L. Martinez-Carrillo, 2001. IPM for Bemisia tabaci:a case study from North America. Crop Protection 20:853–869.

    Google Scholar 

  • Elzen, G. W. and E. G. King, 1999. Periodic release and manipulation of natural enemies, In:T. S. Bellows and T. W. Fisher (eds), Handbook of Biological Control, Academic Press, San Francisco. pp. 253–270

    Google Scholar 

  • Gerling, D., 1990. Natural enemies of white flies:predators and parasitoids. In: D. Gerling (ed), White flies:Their Bionomics, Pest Status and Management, Intercept, Andover, UK. pp. 147–185.

  • Gerling, D., O. Alomar and J. Arno, 2001. Biological control of Bemisia tabaci using predators and parasitoids. Crop Protection 20:779–799.

    Google Scholar 

  • Goolsby, J. A. and M. A. Ciomperlik, 1999. Development of parasitoid inoculated seedling transplants for augmentative biological control of silverleaf white fly (Homoptera:Aleyrodidae). Florida Entomol. 82:532–545.

    Google Scholar 

  • Goolsby, J. A., M. A. Ciomperlik, A. A. Kirk, W. A. Jones, B. C. Legaspi, R. A. Ruiz, D. C. Vacek and L. E. Wendel, 2000. Predictive and empirical evaluation for parasitoids of Bemisia tabaci (Biotype “B”), based on morphological and molecular systematics. In:A. Austin and M. Dowton (eds), Hymenoptera:Evolution, Biodiversity, and Biological Control, Fourth International Hymenopterists Conference, 1999, Canberra, CSIRO Publishing, Collingwood, Victoria, Australia. pp. 347–358.

    Google Scholar 

  • Gould, J. R. and S. E. Naranjo, 1999. Distribution and sampling of Bemisia argentifolii (Homoptera:Aleyrodidae)and Eretmocerus eremicus (Hymenoptera:Aphelinidae) on cantaloupe vines. J. Econ. Entomol 92:402–408.

    Google Scholar 

  • Gurevitch, J. and S. T. Chester, Jr., 1986. Analysis of repeated measures experiments. Ecology 67:251–255.

    Google Scholar 

  • Hagler, J. R., C. Jackson, T. J. Henneberry and J. Gould, 2002. Parasitoid mark-release-recapture techniques-II. Development and application of a protein marking technique for Eretmocerus spp., parasitoids of Bemisia argentifolii. Biocontrol Sci. Technol. 12:661–675.

    Google Scholar 

  • Headrick, D. H., T. S. Bellows, Jr. and T. M. Perring, 1996. Behaviors of female Eretmocerus sp. nr. californicus (Hymenoptera:Aphelinidae)attacking Bemisia argentifolii (Homoptera:Aleyrodidae)on cotton, Gossypium hirsutum, (Malavaceae) and melon, Cucumis melo (Curcurbitaceae). Biol. Control 6:64–75.

    Google Scholar 

  • Hoddle, M. S., J. P. Sanderson and R. G. Van Driesche, 1999. Biological control of Bemisia argentifolii (Hemiptera:Aleyrodidae)on poinsettia with inundative releases of Eretmocerus eremicus (Hymenoptera:Aphelinidae):does varying the weekly release rate affect control?Bull. Entomol. Res. 89:41–51.

    Google Scholar 

  • Hoelmer, K. A., 1998. Comparative eld cage evaluations of top-performing introduced parasitoids in desert cantaloupe, In:T. J. Henneberry, N. C. Toscano, R. M. Faust and J. R. Coppedge (eds), Silverleaf White fly National Research, Action, and Technology Transfer Plan, 1998–2001 (Formerly Sweetpotato White fly, Strain B):First Annual Review of the Second 5-Year Plan, Charleston, South Carolina, February 3-5, 1998. U. S. Department of Agriculture, Agricultural Research Service, 1998-1901, 169 pp.

  • Hofsvang, T. and E. B. Ha ¢ªgvar, 1979. Different introduction methods of Ephedrus cerasicola Stary to control Myzus persicae (Sulzer)in small paprika glasshouses. Z. Ang. Ent. 88:16–23.

    Google Scholar 

  • Huffaker, C. B. (ed. ), 1971. Biological Control. Plenum Press, New York.

    Google Scholar 

  • Jones, W. A., M. A. Ciomperlik and D. A. Wolfenbarger, 1998. Lethal and sublethal effects of insecticides on two parasitoids attacking Bemisia argentifolii (Homoptera: Aleyrodidae). Biol. Control 11:70–76.

    Google Scholar 

  • Jones, W. A., S. M. Greenberg and B. Legaspi, Jr., 1999. The effect of varying Bemisia argentifolii and Eretmocerus mundus ratios on parasitism. BioControl 44:13–28.

    Google Scholar 

  • Mills, N., C. Pickel, S. Mansfield, S. McDougall, R. Buchner, J. Caprile, S. Edstrom, R. Elkins, J. Hasey, K. Kelley, B. Krueger, B. Olson and R. Stocker, 2000. Mass releases of Trichogramma wasps can reduce damage from codling moth. Calif. Agri. 54(6):22–25.

    Google Scholar 

  • Naranjo, S. 2001. Conservation and evaluation of natural enemies in IPM systems for Bemisia tabaci. Crop Protection 20:835–852.

    Google Scholar 

  • Oatman, E. R. 1970. Integration of Phytoseiulus persimilis with native predators for control of the two-spotted spider mite on rhubarb. J. Econ. Entomol. 63:1177–1180.

    Google Scholar 

  • Obrycki, J. J., L. C. Lewis and D. B. Orr, 1997. Augmentative releases of entomophagous species in annual cropping systems. Biol. Control 10:30–36.

    Google Scholar 

  • Oliveira, M. R. V., T. J. Henneberry and P. Anderson, 2001. History, current status, and collaborative research projects for Bemisia tabaci. Crop Protection 20:709–723.

    Google Scholar 

  • Palumbo, J. C., A. R. Horowitz and N. Prabhaker, 2001. Insecticidal control and resistance management for Bemisia tabaci. Crop Protection 20:739–765.

    Google Scholar 

  • Parrella, M. P., K. M. Heinz and L. Nunney, 1992. Biological control through augmentative releases of natural enemies:a strategy whose time has come. Am. Entomol. 38:172–179.

    Google Scholar 

  • Perring, T. M., A. D. Cooper, R. J. Rodriguez, C. A. Farrar and T. S. Bellows, Jr., 1993. Identification of a white fly species by genomic and behavioral studies. Science 259: 74–77.

    PubMed  Google Scholar 

  • Pickett, C. H., F. E. Gilstrap, R. K. Morrison and L. F. Bouse, 1987. Release of predatory mites (Acari:Phytoseiidae)by aircraft for the biological control of spider mites (Acari:Tetranychidae)infesting corn. J. Econ. Entomol. 80:906–910.

    Google Scholar 

  • Powell, D. A., T. S. Bellows, Jr., 1992. Adult longevity, fertility and population growth rates for Bemisia tabaci (Genn. )(Hom., Aleyrodidae)on two host plant species. Environ. Entomol. 21:651–658.

    Google Scholar 

  • Prabhaker, N., N. C. Toscano, S. Castle and T. Henneberry, 1995. Hydroponic bioassay technique to monitor responses of white flies to imidacloprid. In:T. J. Henneberry, N. C. Toscano, R. M. Faust and J. R. Coppedge (eds), 1995 Silverleaf white fly (Formerly Sweetpotato White fly, Strain B ):Supplement to the 5-year National Research and Action Plan, San Diego, California, January 28–31, 1995. U. S. Department of Agriculture-ARS. 305 pp.

  • Prabhaker, N., N. C. Toscano, T. M. Perring, G. Nuessly, K. Kido and R. youngman, 1992. Resistance monitoring of the sweetpotato white fly (Homoptera:Aleyrodidae) in the Imperial Valley of California. J. Econ. Entomol. 85:1063–1068.

    Google Scholar 

  • Ridgeway, R. L. and S. B. Vinson (eds), 1976. Biological Control by Augmentation of Natural Enemies. Plenum Press, New York. 480 pp.

    Google Scholar 

  • Riley, D. G. and M. A. Ciomperlik, 1997. Regional population dynamics of white fly (Homoptera:Aleyrodidae)and associated parasitoids (Hymenoptera:Aphelinidae). Environ. Entomol. 26:1049–1055.

    Google Scholar 

  • Rose, M. and G. Zolnerowich, 1997. Eretmocerus Haldeman (Hymenoptera:Aphelinidae)in the United States, with descriptions of new species attacking Bemisia (Tabaci complex)Homoptera:Aleyrodidae). Proc. Entomol. Soc. Wash. 1–27.

  • Roltsch, W. J., E. R. Andress, K. A. Hoelmer and G. S. Simmons, 2000. Establishment of introduced parasitoids of the silverleaf white fly in Imperial Valley, CA. In:T. J. Henneberry, R. M. Faust, W. A. Jones and T. M. Perring (eds), Supplement to the Third Annual Review of the Second 5-Year National Research and Action Plan, San Diego, California, February 6–8. U. S. Department of Agriculture, Agricultural Research Service, 200 pp.

  • SAS Institute Inc., 1990. SAS/STAT User's Guide. Vol. 1, 2 Vers. 6, Cary North Carolina.

  • Simmons, G. S., 2000. Studies on dispersal of a native parasitoid Eretmocerus eremicus and augmentative biological control of Bemisia tabaci infesting cotton. Ph. D. Dissertation. Department of Entomology, University of Arizona. 225 pp.

    Google Scholar 

  • Simmons, G. S., K. Hoelmer, R. Staten and T. Boratynski, 1995. Biological control of Bemisia in spring melons. In:T. J. Henneberry, N. C. Toscano, R. M. Faust and J. R. Coppedge (eds), 1995 Supplement to the 5-year National Research and Action Plan, San Diego, CA, January 28–31, 1995. U. S. Department of Agriculture, Agricultural Research Service, 1995-2002. 299 pp.

  • Simmons, G. S., K. Hoelmer, R. Staten and T. Boratynski, 1996. Seasonal inoculations in spring melons with parasitoids of Bemisia. In:T. J. Henneberry, N. C. Toscano, R. M. Faust and J. R. Coppedge (eds), 1996 Supplement to the 5-year National Research and Action Plan, San Antonio, Texas, February 4–6, 1996. U. S. Department of Agriculture, Agricultural Research Service, 1996-01, 232 pp.

  • Simmons, G. S., K. Hoelmer, R. Staten and T. Boratynski, 1998. Biological control of silverleaf white fly infesting cantaloupe with large scale releases of exotic parasitoids in the Imperial Valley of California In:T. J. Henneberry, N. C. Toscano, R. M. Faust and J. R. Coppedge (eds), Silverleaf White fly National Research, Action, and Technology Transfer Plan, 1998–2001 (Formerly Sweetpotato White fly Strain B ):First Annual Review of the Second 5-Year Plan, Charleston, South Carolina, February 3-5, 1998. U. S. Department of Agriculture, Agricultural Research Service, 1998-01, 169 pp.

  • Stacey, D. L., 1977. ‘Banker’ plant production of Encarsia formosa Gahn and its use in the control of glasshouse white fly on tomatoes. Pl. Path. 26:63–66.

    Google Scholar 

  • Steel, R. G. D. and J. H. Torrie, 1960. Principles and Procedures of Statistics. McGraw Hill, New York.

    Google Scholar 

  • Tan, W., D. G. Riley and D. A. Wolfenbarger, 1996. Quantification and genetic analysis of bifenthrin resistance in the silverleaf white fly. Southwest. Entomol. 21:265–275.

    Google Scholar 

  • Tonhasca, A. Jr., J. C. Palumbo and D. N. Byrne, 1994. Distribution patterns of Bemisia tabaci (Homoptera:Aleyrodidae)in cantaloupe fields in Arizona. Environ. Entomol. 23:949–954.

    Google Scholar 

  • Varley, C. G., G. R. Gradwell and M. P. Hassell, 1973. Insect Population Ecology, an Analytical Approach. University of California Press, Berkeley. 212 pp.

    Google Scholar 

  • Vet, L. E. M., J. C. van Lenteren and J. Woets, 1980. The parasite-host relationship between Encarsia formosa (Hymenoptera:Aphelinidae)and Trialeurodes vaporariorum (Homoptera:Aleyrodidae). IX. A review of the biological control of the greenhouse white fly with suggestions for future research. Z. Ang. Ent. 90:26–51.

    Google Scholar 

  • Wolfenbarger, D. A. and D. G. Riley, 1994. Toxicity of mixtures of insecticides and insecticides alone against b-strain sweetpotato white fly. In:D. J. Herber and D. A. Richter (eds), Proceedings, Beltwide Cotton Production Conference, San Antonio, TX. pp. 1214–1216.

  • Zolnerowich, G. and M. Rose, 1998. Eretmocerus Haldeman (Hymenoptera:Aphelinidae)imported and released in the United States for control of Bemisia (Tabaci complex)(Homoptera:Aleyrodidae). Proc. Entomol. Soc. Wash. 100:310–323.

    Google Scholar 

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Authors and Affiliations

  1. California Department of Food & Agriculture, Biological Control Program, 3288 Meadowview Rd., CA, USA

    Charles Pickett

  2. USDA-APHIS, 4151 Hwy 86, CA, USA

    Gregory Simmons & Enrique Lozano

  3. USDA-APHIS-PPQ-CPHST, Decision Support & Pest Management Systems Laboratory, 3645, E. Wier Avenue, USA

    Gregory Simmons & John Goolsby

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  1. Charles Pickett
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Pickett, C., Simmons, G., Lozano, E. et al. Augmentative biological control of whiteflies using transplants. BioControl 49, 665–688 (2004). https://doi.org/10.1007/s10526-004-0270-1

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