Abstract
The goal of this study was to compare the environmental factors that determine viability of the gram-negative photosynthesizing bacteria Rhodobacter capsulatus (specifically, light and growth medium composition) and to assess the effect of these factors on the synthesis and composition of lipopolysaccharides released from the cell wall. Depletion of medium resulted in the release of lipopolysaccharides with a truncated polysaccharide fragment. It is concluded that, due to high viability of these bacteria, the main factor that induced lipopolysaccharide release from the cell wall, irrespective of culturing conditions, is cell division rather than cell death.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Adler, K., Simple Tools and Approved Techniques in Cryo-preparation of Biological Samples in SEM and TEM-Investigations, in Electron Microscopy, Megias-Megias, L., Rodriguez-Garcia, M.I., Rios, A., and Arias, J.M., Eds., Granada, Spain, 1992, vol. 3, pp. 51–52.
Cohen-Bazire, G., Sistrom, W.R., and Stanier, R.Y., Kinetic Studies of Pigment Synthesis by Non-Sulfur Purple Bacteria, J. Cellular Comp. Physiol., 1957, vol. 49, no.1, p. 25.
Dierstein, R. and Drews, G., Nitrogen-Limited Continuous Culture of Rhodopseudomonas capsulata Growing Photosynthetically or Heterotrophically under Low Oxygen Tensions, Arch. Microbiol., 1974, vol. 99, pp. 117–128.
Dofferhoff, A.S.M., Esselink, M.T., de Vries-Hospers, H.G., Zanten, A.V., Bom, V.J.J., Weits, J., and Vellenga, E., The Release of Endotoxin from Antibiotic-Treated Escherichia coli and the Production of Tumour Necrosis Factor by Human Monocytes, J. Antimicr. Chemoterapy, 1993, vol. 31, pp. 373–384.
Egorov, N.S., Praktikum po mikrobiologii (A Practical Course on Microbiology), Moscow: Mosk. Gos. Univ., 1976.
Egorova, T.P., Glebovskaya, E.P., and Levenson, V.I., The Use of Colorimetric Method of Determination of Lipopolysaccharide in Immunological Studies, Immunologiya, 1988, no. 4, pp. 79–82.
Engvall, E. and Pesce, A.J., Quantitative Enzyme Immunoassay, Skand. J. Immunol., 1978, vol. 8.
Evans, M.E. and Pollack, M., Effect of Antibiotic Class and Concentration on the Release of Lipopolysaccharide from Escherichia Coli, J. Infect. Dis., 1993, vol. 167, no.6, pp. 1336–1343.
Huijbregts, R.P.H., de Kroon, A.I.P.M., and de Kruijff, B., Topology and Transport of Membrane Lipids in Bacteria, Biochim. Biophys. Acta, 2000, vol. 1469, pp. 43–61.
Janda, J. and Work, E., A Colorimetric Estimation of Lipopolysaccharides, FEBS Lett., 1971, vol. 16, pp. 343–345.
Karkhanis, Y.D., Zeltner, J.Y., Jackson, J.J., and Carlo, D.J., A New and Improved Microassay to Determine 2-Keto-3-Deoxyoctonate in Lipopolysaccharide of Gram-Negative Bacteria, Anal. Biochem., 1978, vol. 85, no.2, pp. 595–601.
Leive, L., Release of Lipopolysaccharide by EDTA Treatment of E. Coli, Biochem. Biophys. Res. Comm., 1965, vol. 21, pp. 290–296.
Levin, J. and Bang, F.B., The Role of Endotoxin in the Extracellular Coagulation of Limulus Blood, Bull. Johns Hopkins Hosp., 1964, vol. 115, pp. 265–274.
Madigan, M.T. and Gest, H., Growth of the Photosynthetic Bacterium Anaerobically in Darkness Supported by “Oxidant-Dependent” Sugar Fermentation, Arch. Microbiol., 1978, vol. 117, no.1, pp. 119–122.
Makhneva, Z.K., Vishnevetskaya, T.A., and Prokhorenko, I.R., The Effect of Isolation Technique on the Yield and Composition of Lipopolysaccharides from Photosynthesizing Bacteria, Prikl. Biokhim. Mikrobiol., 1996, vol. 32, no.4, pp. 444–447.
Ohno, N. and Morrison, D.C., Lipopolysaccharide Interactions with Lysozyme Differentially Effect Lipopolysaccharide Immunostimulatory, Eur. J. Biochem., 1989, vol. 186, pp. 629–636.
Pfeiffer, R., Untersuchungen uber das Choleragift, Z.Hygiene, 1892, vol. 11, pp. 393–412.
Pietsch, K., Weckesser, J., Fischer, U., and Mayer, H., The Lipopolysaccharides of Rhodospirillum rubrum, Rhodospirillum molischianum, and Rhodopila globiformis, Arch. Microbiol., 1990, vol. 154, pp. 433–437.
Rietschel, E.T., Kirikae, T., Schade, F.U., et al., Bacterial Endotoxin: Molecular Relationships of Structure to Activity and Function, FASEB J., 1994, vol. 8, pp. 217–225.
Schultz, J.E. and Weaver, P.F., Fermentation and Anaerobic Respiration by Rhodospirillum rubrum and Rhodopseudomonas capsulata, J. Bacteriol., 1982, vol. 149, pp. 181–190.
Suss, K-H., Arkova, C., Manteuffel, R., and Adler, K., Calvin Cycle Multienzyme Complexes Are Bound to Chloroplast Thylacoid Membranes of Higher Plants in Situ, Proc. Natl. Acad. Sci. USA, 1993, vol. 90, pp. 5514–5518.
Tesch, V.L. and Morrison, D.C., The Interaction of Escherichia coli with Normal Human Serum: Factors Affecting the Capacity of Serum To Mediate Lipopolysaccharide Release, Microb. Pathog., 1988, vol. 4, pp. 175–187.
Tsygankov, A.A. and Gogotov, I.N., Obtaining Biomass of Purple Bacteria, Prikl. Biokhim. Mikrobiol., 1990, vol. 26, no.6, pp. 819–824.
Woese, C.R., Bacterial Evolution, Microbiol. Rev., 1987, vol. 51, pp. 221–271.
Author information
Authors and Affiliations
Additional information
Translated from Izvestiya Akademii Nauk, Seriya Biologicheskaya, No. 2, 2005, pp. 168–173.
Original Russian Text Copyright © 2005 by Zubova, Melzer, Prokhorenko.
Rights and permissions
About this article
Cite this article
Zubova, S.V., Melzer, M. & Prokhorenko, I.R. Effect of environmental factors on the composition of lipopolysaccharides released from the Rhodobacter capsulatus cell wall. Biol Bull Russ Acad Sci 32, 133–137 (2005). https://doi.org/10.1007/s10525-005-0019-0
Received:
Issue Date:
DOI: https://doi.org/10.1007/s10525-005-0019-0