Abstract
Oxidative stress plays an important role in the evolution of aging and life history. High investments in life-history traits and environmental conditions can be associated with increased oxidative stress and aging process. However, to date, most studies that investigated variations in oxidative status were performed with long-lived vertebrates. Studies with short-lived vertebrates in wild are nonexistent. Annual killifishes have the shortest lifespans among vertebrates and inhabit temporary ponds subject to large variations in environmental conditions. In this sense, we investigated whether the high investment in growth and reproduction in a short-lived vertebrate and the large variations in environment has any cost in susceptibility to oxidative stress. We assessed the seasonal variation and the environmental correlates of four different oxidative status markers (lipid peroxidation and activity of the antioxidant enzymes Superoxide Dismutase, Catalase and Glutathione S-Transferase) along the life cycle of wild individuals of the Neotropical annual fish Austrolebias minuano. Males showed reduction in all biomarkers (except proteins) along their life cycle, while females showed increased oxidative stress only in the growth period. In addition, we showed that water physicochemical parameters, habitat structure and presence of co-occurring killifish species influenced the seasonal variation of the biomarkers. A. minuano showed an efficient antioxidant system for most part of their life cycle (mainly in males), suggesting a well-developed oxidative stress regulation system. We also show that annual fish mortality (mainly in males) apparently is not related to oxidative stress. Thus, environmental factors should drive annual fish aging and mortality.
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Acknowledgements
The authors thank the Lagoa do Peixe National Park Administration and ICMBio for the research permits and private landowners for granting access to their properties. The annual killifish collections complied with current Brazilian law (SISBIO 43251-1) and the histological procedures were authorized by the PUCRS Ethic Committee (CEUA PUCRS 8271). L.M. holds a CNPq Research Productivity grant. M.M.P. received a postdoctoral fellowship from CNPq (Grant Number 151152/2018-7). L.E.K.L. received a postdoctoral fellowship from CAPES (Coordination for the Improvement of Higher Level Personnel). R.S.G. received a master and PhD scholarship from CAPES.
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Godoy, R.S., Lanés, L.E.K., Castro, B.D. et al. Oxidative stress resistance in a short-lived Neotropical annual killifish. Biogerontology 21, 217–229 (2020). https://doi.org/10.1007/s10522-019-09855-w
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DOI: https://doi.org/10.1007/s10522-019-09855-w