l-Lysine suppresses myofibrillar protein degradation and autophagy in skeletal muscles of senescence-accelerated mouse prone 8
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Sarcopenia is a condition of the loss of muscle mass that is associated with aging and that increases the risk for bedridden state, thereby warranting studies of interventions that attenuate sarcopenia. Here the effects of 2-month dietary l-lysine (Lys) supplementation (1.5–3.0 %) on myofibrillar protein degradation and major proteolytic systems were investigated in senescence-accelerated mouse prone 8 (SAMP8). At 36 weeks of age, skeletal muscle and lean body mass was reduced in SAMP8 when compared with control senescence-accelerated mouse resistant 1 (SAMR1). The myofibrillar protein degradation, which was evaluated by the release of 3-methylhistidine, was stimulated in SAMP8, and the autophagy activity, which was evaluated by light chain 3-II, was stimulated in the skeletal muscle of SAMP8. The activation of ubiquitin-proteasome system was not observed in the muscles of SAMP8. However, myofibrillar protein degradation and autophagic activity in skeletal muscles of SAMP8 were suppressed by dietary intake of 3.0 % Lys. The present data indicate that myofibrillar protein degradation by bulk autophagy is stimulated in the skeletal muscles of SAMP8 and that dietary Lys supplementation attenuates sarcopenia in SAMP8 by suppressing autophagic proteolysis.
KeywordsSarcopenia Autophagy Ubiquitin proteasome system Skeletal muscle Lysine
Light chain 3
Mammalian target of rapamycin
Mitochondrial E3 ubiquitin protein ligase 1
Muscle ring-finger protein 1
Peroxisome proliferator activated receptor γ co-activator 1α
Quantitative reverse transcription PCR
Senescence-accelerated mouse prone 8
Senescence-accelerated mouse resistant 1
p70 ribosomal protein S6 kinase 1
Eukaryotic initiation factor 4E binding protein 1
Amino acids were provided by Ajinomoto Co., Inc. Primer sequences for atrogin-1 and MuRF1 were provided by Professor Takeshi Nikawa, Tokushima University, Japan.
This work was supported by the Sasakawa Scientific Research Grant from The Japan Science Society (Grant No. 27-414).
Compliance with ethical standards
Conflict of interest
We declare no conflict of interest related to this study.
Human subject is not involved in this study.
Research involving Animals
Male SAMP8 (15-week-old, 27–35 g, n = 17) and SAMR1 (15-week-old, 31–35 g, n = 6) were purchased from Japan SLC, Inc. (Shizuoka, Japan). All animal protocols were approved by the Iwate University Animal Research Committee and were performed in compliance with the Guidelines for Animal Experiments of Iwate University (approval number, A201517).
- Cruz-Jentoft AJ, Baeyens JP, Bauer JM, Boirie Y, Cederholm T, Landi F, Martin FC, Michel JP, Rolland Y, Schneider SM, Topinková E, Vandewoude M, Zamboni M (2010) Sarcopenia: European consensus on definition and diagnosis: report of the European working group on sarcopenia in older people. Age Ageing 39:412–423CrossRefPubMedPubMedCentralGoogle Scholar
- Ghosh S, Lertwattanarak R, Lefort N, Molina-Carrion M, Joya-Galeana J, Bowen BP, Garduno-Garcia Jde J, Abdul-Ghani M, Richardson A, DeFronzo RA, Mandarino L, Van Remmen H, Musi N (2011) Reduction in reactive oxygen species production by mitochondria from elderly subjects with normal and impaired glucose tolerance. Diabetes 60:2051–2060CrossRefPubMedPubMedCentralGoogle Scholar
- Hughes DC, Stewart CE, Sculthorpe N, Dugdale HF, Yousefian F, Lewis MP, Sharples AP (2016) Testosterone enables growth and hypertrophy in fusion impaired myoblasts that display myotube atrophy: deciphering the role of androgen and IGF-I receptors. Biogerontology 17:619–639CrossRefPubMedGoogle Scholar
- Joseph AM, Adhihetty PJ, Buford TW, Wohlgemuth SE, Lees HA, Nguyen LM, Aranda JM, Sandesara BD, Pahor M, Manini TM, Marzetti E, Leeuwenburgh C (2012) The impact of aging on mitochondrial function and biogenesis pathways in skeletal muscle of sedentary high- and low-functioning elderly individuals. Aging Cell 11:801–809CrossRefPubMedPubMedCentralGoogle Scholar
- Reeves PG, Nielsen FH, Fahey GC Jr (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123:1939–1951Google Scholar
- Sandri M, Barberi L, Bijlsma AY, Blaauw B, Dyar KA, Milan G, Mammucari C, Meskers CG, Pallafacchina G, Paoli A, Pion D, Roceri M, Romanello V, Serrano AL, Toniolo L, Larsson L, Maier AB, Muñoz-Cánoves P, Musarò A, Pende M, Reggiani C, Rizzuto R, Schiaffino S (2013) Signalling pathways regulating muscle mass in ageing skeletal muscle: the role of the IGF1-Akt-mTOR-FoxO pathway. Biogerontology 14:303–323CrossRefPubMedGoogle Scholar