Biogerontology

, Volume 15, Issue 2, pp 113–127 | Cite as

The effects of pectins on life span and stress resistance in Drosophila melanogaster

  • Mikhail Shaposhnikov
  • Dmitrii Latkin
  • Ekaterina Plyusnina
  • Lyubov Shilova
  • Anton Danilov
  • Sergey Popov
  • Alexander Zhavoronkov
  • Yuri Ovodov
  • Alexey Moskalev
Research Article

Abstract

The composition of diet is one of the major determining factors for lifespan. The dietary pectins are known to have anti-inflammatory properties and may influence aging and longevity. Here we demonstrate the lifespan-extending effect of the low methyl esterified (LM) commercial pectins CU701 and AU701 in wild-type strain of Drosophila melanogaster. The high methyl esterified (HM) pectin CU201 did not affect lifespan. LM pectin did not increase lifespan of males with a mutation in the Toll adaptor Myd88 gene and in both males and females with a mutation in the NF-κB ortholog Relish. LM pectin CU701 increased imagoes survival in stress conditions (oxidation, hyperthermia and starvation). However, the fertility of LM and HM pectins treated flies decreased. The treatment of the imagoes with LH and HM pectins induced the activation of whole-body expression of genes involved in DNA repair (D-GADD45, mei-9, spn-B), apoptosis (wrinkled/hid) and heat shock response (hsp70Aa). In contrast, the expression of proinflammatory PARP-1 gene decreased. In the intestines LH and HM pectins induced the mRNA expression of the NF-κB-dependent antimicrobial genes Defensin, Drosomycin and Metchnikowin. These results indicate that the observed lifespan-extending effect of the LM pectins may be mediated by intracellular pathways that involve NF-κB signalling and activation of stress resistance genes.

Keywords

Drosophila melanogaster Pectins Lifespan NF-κB Anti-inflammatory activity 

Notes

Acknowledgments

We thank the anonymous reviewers for useful comments and suggestions. We are grateful to Drosophila Stock Center (Indiana University, Bloomington, Indiana, USA) for providing the D. melanogaster strains. The study was supported by grant from the Russian Foundation for Basic Research (11-04-12110-ofi-m-2011) and by the Programs of the Presidium of the Ural Branch of the RAS (grants # 12-П-4-1033 and 12-П-4-1023).

Supplementary material

10522_2013_9484_MOESM1_ESM.doc (2.4 mb)
Supplementary material 1 (DOC 2468 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Mikhail Shaposhnikov
    • 1
    • 2
  • Dmitrii Latkin
    • 3
  • Ekaterina Plyusnina
    • 1
    • 2
  • Lyubov Shilova
    • 1
  • Anton Danilov
    • 1
  • Sergey Popov
    • 3
  • Alexander Zhavoronkov
    • 4
  • Yuri Ovodov
    • 3
  • Alexey Moskalev
    • 1
    • 2
    • 5
  1. 1.Laboratory of Molecular Radiobiology and Gerontology, Institute of BiologyKomi Science Center of Russian Academy of SciencesSyktyvkarRussia
  2. 2.Syktyvkar State UniversitySyktyvkarRussia
  3. 3.Institute of Physiology, Komi Science CentreThe Urals Branch of the Russian Academy of SciencesSyktyvkarRussia
  4. 4.Center for Pediatric Hematology, Oncology and ImmunologyMoscowRussia
  5. 5.Moscow Institute of Physics and TechnologyDolgoprudnyRussia

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