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Differential expression of Toll-like receptor 2 and 4 on peritoneal leukocyte populations from long-lived and non-selected old female mice

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Abstract

The aim of the present study was to determine Toll-like receptor (TLR)-2 and TLR-4 membrane expression on the major peritoneal leukocyte populations throughout the aging process, including subjects that had achieved exceptional longevity. ICR (CD1) female mice of different ages: adult (44 ± 4 weeks), old (69 ± 4), very old (92 ± 4) and extreme long-lived (125 ± 4), were used. Peritoneal leukocytes were collected, and percentages of CD11b, CD11c, CD3CD4, CD3CD8 and CD19 cells present in the samples were analysed, as well as the expression of TLR-2 and TLR-4 on them, by flow cytometry. The results showed increased TLR expression on CD11b+ cells from animals at very old ages and especially in the extreme long-lived. Old subjects showed lower percentage of CD11c+ cells, but no age-related changes were found in the TLR expression on these cells. TLRs on CD3CD4+ and CD3CD8+ cells from very old animals were increased as compared to the adults, whereas long-lived subjects showed preserved levels. However, TLR expression on CD19+ cells was higher in long-lived individuals with respect to subjects at all the other younger ages. These data suggest that differential age-related changes in the expression of TLR-2 and TLR-4 on leukocyte populations from long-lived and non-selected younger old mice could contribute to a different age-related immune remodelling in long-lived subjects, which could allow better preservation of their immune responses.

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Acknowledgments

This work was supported by MCINN (BFU2008-04336), Research Group of UCM (910379) and RETICEF (RD06/0013/003).

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Authors and Affiliations

  1. Departamento de Fisiología (Fisiología Animal II), Facultad de Ciencias Biológicas, Universidad Complutense, 28040, Madrid, España

    Lorena Arranz, Nuria M. De Castro, Isabel Baeza & Mónica De la Fuente

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  1. Lorena Arranz
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  2. Nuria M. De Castro
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  3. Isabel Baeza
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  4. Mónica De la Fuente
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Correspondence to Mónica De la Fuente.

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Arranz, L., De Castro, N.M., Baeza, I. et al. Differential expression of Toll-like receptor 2 and 4 on peritoneal leukocyte populations from long-lived and non-selected old female mice. Biogerontology 11, 475–482 (2010). https://doi.org/10.1007/s10522-010-9270-y

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  • DOI: https://doi.org/10.1007/s10522-010-9270-y

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