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Glyoxalase I activity and immunoreactivity in the aging human lens

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Abstract

Glyoxalase I (GLOI) is the first enzyme of the glyoxalase system that catalyzes the metabolism of reactive dicarbonyls, such as methylglyoxal (MGO). During aging and cataract development, human lens proteins are chemically modified by MGO, which is likely due to inadequate metabolism of MGO by the glyoxalase system. In this study, we have determined the effect of aging on GLOI activity and the immunoreactivity and morphological distribution of GLOI in the human lens. A monoclonal antibody was developed against human GLOI. GLOI immunoreactivity was strongest in the anterior epithelial cells and weaker in rest of the lens. Cultured human lens epithelial cells showed immunostaining throughout the cytoplasm. In the human lens, GLOI activity and immunoreactivity both decreased with age. We believe that this would lead to promotion of MGO-modification in aging lens proteins.

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Acknowledgments

This study was supported from NIH grants R01EY-016219 and R01EY-09912 (RHN), P30EY-11373 (Visual Sciences Research Center of CWRU), Research to Prevent Blindness, NY, and the Ohio Lions Eye Research Foundation. We thank Catherine Doller at the Visual Sciences Research Center for help with immunostaining experiments and Santosh Kanade, Mahesha Gangadhariah for help with the GLOI activity assay.

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Authors and Affiliations

  1. Department of Ophthalmology and Visual Sciences, Case Western Reserve University, Pathology Building 311, 2085 Adelbert Road, Cleveland, OH, 44106, USA

    Maneesh Mailankot, Smitha Padmanabha, NagaRekha Pasupuleti & Ram H. Nagaraj

  2. Visual Sciences Research Center, Case Western Reserve University, Cleveland, OH, 44106, USA

    Denice Major & Scott Howell

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  1. Maneesh Mailankot
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  2. Smitha Padmanabha
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  3. NagaRekha Pasupuleti
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  4. Denice Major
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  5. Scott Howell
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  6. Ram H. Nagaraj
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Correspondence to Ram H. Nagaraj.

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Mailankot, M., Padmanabha, S., Pasupuleti, N. et al. Glyoxalase I activity and immunoreactivity in the aging human lens. Biogerontology 10, 711–720 (2009). https://doi.org/10.1007/s10522-009-9218-2

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  • DOI: https://doi.org/10.1007/s10522-009-9218-2

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