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Hsp70 prevents disuse muscle atrophy in senescent rats

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Abstract

This study determined the effects of heat shock protein 70 (Hsp70) overexpression on disuse muscle atrophy in senescent rats. Solei of young and senescent rats were co-injected with Hsp70 plus a nuclear factor kappa B (NF-κΒ) reporter plasmid. After 4 days, the hind limbs of half the young and senescent rats were immobilized for 6 days with the remainder serving as weight bearing controls. Hsp70 protein levels and cross-sectional area decreased in both groups (~20%) after immobilization. Atrophy was prevented in those fibers overexpressing Hsp70. NF-κΒ activity increased in the soleus of both young (three-fold) and senescent (five-fold) animals after immobilization and was prevented by Hsp70 overexpression. Inhibitor of κΒ decreased in young (~30%) and senescent (~10%) animals with immobilization and returned to normal with Hsp70. Heat shock protein 70 overexpression prevents disuse atrophy in senescent rats, possibly through suppression of the NF-κB pathway.

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Acknowledgments

We thank Sarah Senf for critical reading and editorial comments.

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Authors and Affiliations

  1. Department of Applied Physiology and Kinesiology, University of Florida, P.O. Box 118205, Gainesville, FL, 32611, USA

    Stephen Dodd, Brian Hain & Andrew Judge

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  1. Stephen Dodd
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  2. Brian Hain
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  3. Andrew Judge
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Correspondence to Stephen Dodd.

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Dodd, S., Hain, B. & Judge, A. Hsp70 prevents disuse muscle atrophy in senescent rats. Biogerontology 10, 605–611 (2009). https://doi.org/10.1007/s10522-008-9203-1

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