Microarray analysis reveals similarity between CD8+CD28− T cells from young and elderly persons, but not of CD8+CD28+ T cells

Lazuardi, Lutfan; Herndler-Brandstetter, Dietmar; Brunner, Stefan; Laschober, Gerhard T.; Lepperdinger, Günter; Grubeck-Loebenstein, Beatrix

doi:10.1007/s10522-008-9167-1

Research Article
Published: 27 August 2008

Microarray analysis reveals similarity between CD8⁺CD28⁻ T cells from young and elderly persons, but not of CD8⁺CD28⁺ T cells

Lutfan Lazuardi¹,
Dietmar Herndler-Brandstetter¹,
Stefan Brunner¹,
Gerhard T. Laschober¹,
Günter Lepperdinger¹ &
Beatrix Grubeck-Loebenstein¹

Biogerontology volume 10, pages 191–202 (2009)Cite this article

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Abstract

We isolated highly purified CD8⁺CD28⁺ and CD8⁺CD28⁻ T cell populations from healthy young and elderly persons for gene expression profiling using Affymetrix oligonucleotide microarrays. We demonstrate that the gene expression profile of CD8⁺CD28⁻ T cells is very similar in young and elderly persons. In contrast, CD8⁺CD28⁺ in elderly differ from CD8⁺CD28⁺ in young persons. Hierarchical clustering revealed that CD8⁺CD28⁺ in elderly are located between CD8⁺CD28⁺ in young and CD8⁺CD28⁻ (young and old) T cells regarding their differentiation state. Our study demonstrates a dichotomy of gene expression levels between CD8⁺CD28⁺ T cells in young and elderly persons but a similarity between CD8⁺CD28⁻ T cells in young and elderly persons. As CD8⁺CD28⁺ T cells from elderly and young persons are distinct due to a different composition of the population, these results suggest that the gene expression profile does not depend on chronological age but depends on the differentiation state of the individual cell types.

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Acknowledgements

The authors thank Michael Keller and Brigitte Jenewein for collecting blood samples. This work was supported by the Austrian Science Fund (Projects S9308-B05 and S9309-B05

).

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Institute for Biomedical Aging Research of the Austrian Academy of Sciences, Rennweg 10, 6020, Innsbruck, Austria
Lutfan Lazuardi, Dietmar Herndler-Brandstetter, Stefan Brunner, Gerhard T. Laschober, Günter Lepperdinger & Beatrix Grubeck-Loebenstein

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Lutfan Lazuardi
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Dietmar Herndler-Brandstetter
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Stefan Brunner
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Gerhard T. Laschober
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Günter Lepperdinger
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Beatrix Grubeck-Loebenstein
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Correspondence to Beatrix Grubeck-Loebenstein.

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Lazuardi, L., Herndler-Brandstetter, D., Brunner, S. et al. Microarray analysis reveals similarity between CD8⁺CD28⁻ T cells from young and elderly persons, but not of CD8⁺CD28⁺ T cells. Biogerontology 10, 191–202 (2009). https://doi.org/10.1007/s10522-008-9167-1

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Received: 28 May 2008
Accepted: 04 August 2008
Published: 27 August 2008
Issue Date: April 2009
DOI: https://doi.org/10.1007/s10522-008-9167-1

Keywords

Aging
Humans
CD8⁺ T cell
Gene expression