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Estrogen receptor α expression in mice kidney shows sex differences during aging

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Abstract

We have used semi quantitative RT-PCR and western blotting for the analysis of expression of estrogen receptor (ER) α and βmRNA and protein in the kidney of adult and old mice of both sexes. Uterus, a well-known target for estrogen action, was used for comparison. As compared to adult, the expression of both ER α mRNA and protein of old mice decreased in male but increased in female. However, unlike uterus, neither ERβ mRNA nor protein could be detected in the kidney of adult or old mice. Thus the present data reveal that the expression of ERα in mice kidney shows sex differences during aging.

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Abbreviations

βME:

βmercaptoethanol

ECL:

enhanced chemiluminescence

EDTA:

ethylenediaminetetraacetic acid

ER:

Estrogen receptor

PAGE:

polyacrylamide gel electrophoresis

PBS:

phosphate buffered saline

PCR:

polymerase chain reaction

PMSF:

phenyl methyl sulphonyl fluoride

PVDF:

polyvinyl difluoride

RT:

reverse transcriptase

SDS:

sodium dodecyl sulphate

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Authors and Affiliations

  1. Biochemistry and Molecular Biology Laboratory, Center of Advanced Study in Zoology, Banaras Hindu University, Varanasi, 221005, India

    P. K. Sharma & M. K. Thakur

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  1. P. K. Sharma
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  2. M. K. Thakur
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Correspondence to M. K. Thakur.

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Sharma, P.K., Thakur, M.K. Estrogen receptor α expression in mice kidney shows sex differences during aging. Biogerontology 5, 375–381 (2004). https://doi.org/10.1007/s10522-004-3191-6

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  • DOI: https://doi.org/10.1007/s10522-004-3191-6

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