, Volume 5, Issue 5, pp 317–325 | Cite as

D-Galactose-caused life shortening in Drosophila melanogaster and Musca domestica is associated with oxidative stress

  • Xu Cui
  • Luning Wang
  • Pingping Zuo
  • Zhitao Han
  • Zhengyu Fang
  • Wenbin Li
  • Jiankang Liu


D-Galactose causes aging acceleration in different animal models but the mechanism is unclear. In the present study, we investigated the effects of D-galactose on lifespan and oxidative stress biomarkers in the fruit fly (Drosophila melanogaster) and housefly (Musca domestica). D-Galactose was added to drinking water (20 mg/ml) for housefly and to culture medium (6.5%) for fruit fly from 24 h after emergence. Oxidative stress was estimated by measuring the activity of Cu–Zn-superoxide dismutase (SOD) and the levels of lipid peroxidation products, namely malondialdehyde (MDA) and lipofuscin in housefly brain (male) and in fruit fly (male and female). D-Galactose caused a significant decrease in mean lifespan (by 12.6% of male and 15.9% of female) and maximum lifespan (by 12.9% of male and 17.1% of female) in fruit fly, and also a significant decrease in mean lifespan (by 27.1% of male, 19.8% of female) and maximum lifespan (by 27.1% of male, 21.9% of female) in housefly. MDA and lipofuscin increased with age in fruit fly and in housefly brains while change of the SOD activity showed a biphasic shape with age. D-Galactose caused a significant increase in MDA and lipofuscin and decrease in SOD activity in the age-matched fruit flies and houseflies. These data indicate that D-galactose shortens the lifespan of the two different fly species and that the life shortening effect is associated with an increase in oxidative stress.

Cu–Zn-superoxide dismutase -Galactose Drosophila melanogaster lifespan lipofuscin malondialdehyde Musca domestica 


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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Xu Cui
    • 1
    • 2
  • Luning Wang
    • 2
  • Pingping Zuo
    • 1
  • Zhitao Han
    • 2
  • Zhengyu Fang
    • 2
  • Wenbin Li
    • 2
  • Jiankang Liu
    • 3
    • 4
    • 4
  1. 1.Chinese Academy of Medical Science, Peking Union Medical CollegeInstitute of Basic Medical SciencesChina
  2. 2.Chinese PLA General HospitalInstitute of GerontologyChina
  3. 3.Chinese Academy of SciencesInstitute for Nutritional SciencesChina
  4. 4.Children's Hospital Oakland Research InstituteUSA

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