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Effect of Pyridoxine Derivative B6NO on Transcription Factor Nrf2 Activity and Cytotoxic Properties of Doxorubicin In Vitro

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The effect of a new pyridoxine derivative B6NO on doxorubicin cytotoxicity and Nrf2-dependent cellular processes in vitro was studied. Antioxidant B6NO enhances the cytotoxic effect of doxorubicin on tumor cells, which is associated with G2/M cell division arrest and an increase in activity of proapoptotic enzyme caspase-3. The antioxidant promotes intracellular accumulation and nuclear translocation of Nrf2 transcription factor in non-tumor and tumor cells. In non-tumor cells, B6NO increases the expression of antioxidant system proteins and reduces ROS generation in the presence of doxorubicin. In tumor cells, no activation of Nrf2-dependent processes occurs under the action of the antioxidant. Our findings demonstrate the prospect of further studies of pyridoxine derivatives as antioxidants to reduce adverse reactions during chemotherapy.

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Authors and Affiliations

  1. Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry, Russian Academy of Sciences, Chernogolovka, Moscow Region, Russia

    A. A. Balakina, V. I. Amozova, T. R. Prikhodchenko, T. S. Stupina & D. V. Mishchenko

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  1. A. A. Balakina
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  2. V. I. Amozova
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  3. T. R. Prikhodchenko
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  4. T. S. Stupina
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  5. D. V. Mishchenko
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Correspondence to A. A. Balakina.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 265-275, December, 2023

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Balakina, A.A., Amozova, V.I., Prikhodchenko, T.R. et al. Effect of Pyridoxine Derivative B6NO on Transcription Factor Nrf2 Activity and Cytotoxic Properties of Doxorubicin In Vitro. Bull Exp Biol Med 176, 687–696 (2024). https://doi.org/10.1007/s10517-024-06091-2

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  • DOI: https://doi.org/10.1007/s10517-024-06091-2

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