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A Model of Type II Collagen-Induced Spondylitis and Arthritis in F1 Hybrid Male Mice

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In this study, we tested a new model of ankylosing spondylitis in order to determine its histological and radiological features needed to investigate peripheral arthritis, spondylitis, and formation of the new bone tissues. F1 hybrid male mice (BALB/c×DBA/1), a progeny of spondylitis-susceptible BALB/c male mice and rheumatoid arthritis-susceptible DBA/1 female mice, were immunized intraperitoneally with bovine type II collagen (CII) mixed with adjuvant dimethyldioctadecylammonium bromide. Radiological and histological studies were performed at the peak of swelling, redness, and stiffness. The incidence of peripheral arthritis and spondylitis induced by CII in F1 hybrid mice were 66 and 62%, respectively. X-ray examination revealed bone erosion and spondylitis in the peripheral joints, as well as the formation of new bone tissues in the coccygeal vertebrae and between LIII and LIV vertebrae. The histological study showed lymphocyte and plasma cell infiltration, capillary dilation, congestion, and endochondral ossification of the lumbar vertebrae. This novel model of CII-induced spondylitis in F1 hybrid mice provoked axial and peripheral arthritides inducing chronic inflammation. In this model, the formation of new bone tissue in the stiff spine is characterized by endochondral ossification. The advanced model is an additional and valuable tool for investigation of the autoimmune reactions in spondylitis.

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References

  1. Daikh DI, Chen PP. Advances in managing ankylosing spondylitis. F1000Prime Rep. 2014;6:78. doi: https://doi.org/10.12703/P6-78

  2. Voruganti A, Bowness P. New developments in our understanding of ankylosing spondylitis pathogenesis. Immunology. 2020;161(2):94-102. doi: https://doi.org/10.1111/imm.13242

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Laval SH, Timms A, Edwards S, Bradbury L, Brophy S, Milicic A, Rubin L, Siminovitch KA, Weeks DE, Calin A, Wordsworth BP, Brown MA. Whole-genome screening in ankylosing spondylitis: evidence of non-MHC genetic-susceptibility loci. Am. J. Hum. Genet. 2001;68(4):918-926. doi: https://doi.org/10.1086/319509

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Glant TT, Mikecz K. Proteoglycan aggrecan-induced arthritis: a murine autoimmune model of rheumatoid arthritis. Methods Mol. Med. 2004;102:313-338. doi: https://doi.org/10.1385/1-59259-805-6:313

    Article  CAS  PubMed  Google Scholar 

  5. Roces CB, Khadke S, Christensen D, Perrie Y. Scale-Independent Microfluidic Production of Cationic Liposomal Adjuvants and Development of Enhanced Lymphatic Targeting Strategies. Mol. Pharm. 2019;16(10):4372-4386. doi: https://doi.org/10.1021/acs.molpharmaceut.9b00730

    Article  CAS  PubMed  Google Scholar 

  6. Martin-Bertelsen B, Korsholm KS, Roces CB, Nielsen MH, Christensen D, Franzyk H, Yaghmur A, Foged C. Nano-Self-Assemblies Based on Synthetic Analogues of Mycobacterial Monomycoloyl Glycerol and DDA: Supramolecular Structure and Adjuvant Efficacy. Mol. Pharm. 2016;13(8):2771-2781. doi: https://doi.org/10.1021/acs.molpharmaceut.6b00368

    Article  CAS  PubMed  Google Scholar 

  7. Siu AL; U.S. Preventive Services Task Force. Screening for Breast Cancer: U.S. Preventive Services Task Force Recommendation Statement. Ann. Intern. Med. 2016;164(4):279-296. doi: https://doi.org/10.7326/M15-2886

  8. Aloke C, Ibiam UA, Orji OU, Ugwuja EI, Ezeani NN, Aja PM, Obasi NA. Anti-arthritic potential of ethanol and aqueous extracts of stem bark of Cleistopholis patens on complete Freund’s adjuvant-induced rheumatoid arthritis in rats. J. Ayurveda Integr. Med. 2021;12(1):28-34. doi: https://doi.org/10.1016/j.jaim.2018.12.009

    Article  CAS  PubMed  Google Scholar 

  9. Sheng Z, Zeng J, Huang W, Li L, Li B, Lv C, Yan F. Comparison of therapeutic efficacy and mechanism of paclitaxel alone or in combination with methotrexate in a collagen-induced arthritis rat model. Z. Rheumatol. 2022;81(2):164-173. doi: https://doi.org/10.1007/s00393-020-00940-x

    Article  CAS  PubMed  Google Scholar 

  10. Tan YK, Li H, Allen JC Jr, Thumboo J. Anti-cyclic citrullinated peptide but not rheumatoid factor is associated with ultrasound-detected bone erosion among rheumatoid arthritis patients with at least moderate disease activity. Int. J. Rheum. Dis. 2020;23(10):1337-1343. doi: https://doi.org/10.1111/1756-185X.13933

    Article  CAS  PubMed  Google Scholar 

  11. Brunet SC, Finzel S, Engelke K, Boyd SK, Barnabe C, Manske SL. Bone changes in early inflammatory arthritis assessed with High-Resolution peripheral Quantitative Computed Tomography (HR-pQCT): A 12-month cohort study. Joint Bone Spine. 2021;88(1):105065. doi: https://doi.org/10.1016/j.jbspin.2020.07.014

    Article  PubMed  Google Scholar 

  12. Farooq SM, Kumar A, Ashour HM. Eye-mediated immune tolerance to Type II collagen in arthritis-prone strains of mice. J. Cell. Mol. Med. 2014;18(12):2512-2518. doi: https://doi.org/10.1111/jcmm.12376

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Häyrynen J, Kärkkäinen M, Kononoff A, Arstila L, Elfving P, Niinisalo H, Savolainen E, Kautiainen H, Risteli J, Kaipiainen-Seppänen O, Koivula MK. Automated immunoassays for the autoantibodies to carbamylated or citrullinated telopeptides of type I and II collagens. Clin. Chem. Lab. Med. 2015;53(9):1375-1380. doi: https://doi.org/10.1515/cclm-2014-0683

    Article  CAS  PubMed  Google Scholar 

  14. Viljanen J, Lönnblom E, Ge C, Yang J, Cheng L, Aldi S, Cai W, Kastbom A, Sjöwall C, Gjertsson I, Holmdahl R, Kihlberg J. Synthesis of an Array of Triple-Helical Peptides from Type II Collagen for Multiplex Analysis of Autoantibodies in Rheumatoid Arthritis. ACS Chem. Biol. 2020;15(9):2605-2615. doi: https://doi.org/10.1021/acschembio.0c00680

    Article  CAS  PubMed  Google Scholar 

  15. Manivel VA, Mullazehi M, Padyukov L, Westerlind H, Klareskog L, Alfredsson L, Saevarsdottir S, Rönnelid J. Anticollagen type II antibodies are associated with an acute onset rheumatoid arthritis phenotype and prognosticate lower degree of inflammation during 5-years follow-up. Ann. Rheum. Dis. 2017;76(9):1529-1536. doi: https://doi.org/10.1136/annrheumdis-2016-210873

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Allergy and Clinical Immunology Research Centre, the First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China

    J. M. Liu, J. H. Zhao, Y. Wang, W. Liu & L. Zhou

  2. Jinzhou Palmtop Cloud Biotechnology Co., Ltd., Jinzhou, Liaoning, China

    J. M. Liu

  3. Department of Radiology, the First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China

    X. L. Zhang

  4. Department of Pathology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China

    L. Yang

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  1. J. M. Liu
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  2. J. H. Zhao
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  3. Y. Wang
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  4. W. Liu
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  5. X. L. Zhang
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  6. L. Yang
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  7. L. Zhou
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Corresponding author

Correspondence to L. Zhou.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 175, No. 6, pp. 755-762, June, 2023

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Liu, J.M., Zhao, J.H., Wang, Y. et al. A Model of Type II Collagen-Induced Spondylitis and Arthritis in F1 Hybrid Male Mice. Bull Exp Biol Med 175, 794–800 (2023). https://doi.org/10.1007/s10517-023-05949-1

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  • DOI: https://doi.org/10.1007/s10517-023-05949-1

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