We studied the influence of epiphysectomy and administration of melatonin to epiphysectomized outbred white rats on the level and daily dynamics of damage to the genetic material of developing male germ cells. Epiphysectomy leads to an increase in the level of damage in the DNA structure and the disappearance of the circadian rhythm of the activity of repair enzyme PARP-1 and the apoptosis-inducing enzyme caspase-3. The administration of melatonin to animals after epiphysectomy reduces the level of DNA damage, restores the circadian rhythm of activity of PARP-1 and caspase-3. These fundings suggest that melatonin can indirectly protect DNA of maturing male gametes.
Similar content being viewed by others
References
Chernykh VB, Solovova OA. Male infertility: genetic focus on actual problem. Consilium Medicum. 2019;21(7):19-24. Russian. doi: https://doi.org/10.26442/20751753.2019.7.190517
Krausz C, Riera-Escamilla A. Genetics of male infertility. Nat. Rev. Urol. 2018;15(6):369-384. doi: https://doi.org/10.1038/s41585-018-0003-3
Bisht S, Faiq M, Tolahunase M, Dada R. Oxidative stress and male infertility. Nat. Rev. Urol. 2017;14(8):470-485. doi: https://doi.org/10.1038/nrurol.2017.69
Kirilenko EA, Onopko VF. Oxidative stress and male fertility: modern view on the problem. Acta Biomedica Scientifica. 2017;2(2):102-108. Russian. doi: https://doi.org/10.12737/article_59a614fd84d146.40261567
Boga JA, Caballero B, Potes Y, Perez-Martinez Z, Reiter RJ, Vega-Naredo I, Coto-Montes A. Therapeutic potential of melatonin related to its role as an autophagy regulator: A review. J. Pineal Res. 2019;66(1):e12534. doi: https://doi.org/10.1111/jpi.12534
Majidinia M, Sadeghpour A, Mehrzadi S, Reiter RJ, Khatami N, Yousefi B. Melatonin: A pleiotropic molecule that modulates DNA damage response and repair pathways. J. Pineal Res. 2017;63(1). doi: https://doi.org/10.1111/jpi.12416
Pourhanifeh MH, Mahdavinia M, Reiter RJ, Asemi Z. Potential use of melatonin in skin cancer treatment: A review of current biological evidence. J. Cell. Physiol. 2019;234(8):12 142-12 148. doi: https://doi.org/10.1002/jcp.28129
Yu K, Deng SL, Sun TC, Li YY, Liu YX. Melatonin regulates the synthesis of steroid hormones on male reproduction: A review. Molecules. 2018;23(2):447. doi: https://doi.org/10.3390/molecules23020447
Arav VI, Sych VF, Zheleznyak EV. Effect of pinealectomy on circadian rhythm of spermatogenesis. Bull. Exp. Biol. Med. 2003;136(6):604-606. doi: https://doi.org/10.1023/B:BEBM.0000020216.01084.da
Rodriguez C, Mayo JC, Sainz RM, Antolín I, Herrera F, Martín V, Reiter RJ. Regulation of antioxidant enzymes: a significant role for melatonin. J. Pineal Res. 2004;36(1):1-9. doi: https://doi.org/10.1046/j.1600-079x.2003.00092.x
Arav VI, Slesarev SM, Slesareva EV. A method for extirpation of the pineal gland in albino rats. Bull. Exp. Biol. Med. 2008;146(3):382-384. doi: https://doi.org/10.1007/s10517-008-0284-2
Mehraein F, Negahdar F. Morphometric evaluation of seminiferous tubules in aged mice testes after melatonin administration. Cell J. 2011;13(1):1-4.
Schlatt S, Ehmcke J. Regulation of spermatogenesis: an evolutionary biologist’s perspective. Semin. Cell Dev. Biol. 2014;29:2-16. doi: https://doi.org/10.1016/j.semcdb.2014.03.007
Slesareva EV, Slesarev SM. Apoptosis of testicular germinal tissue in condition disorders of the nervous and epiphyseal regulations. Ul’yanov. Med. Biol. Zh. 2013;(3):119-123. Russian.
Reiter RJ, Tan DX, Mayo JC, Sainz RM, Leon J, Czarnocki Z. Melatonin as an antioxidant: biochemical mechanisms and pathophysiological implications in humans. Acta Biochim. Pol. 2003;50(4):1129-1146.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 175, No. 6, pp. 739-743, June, 2023
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Slesarev, S.M., Okaemova, A.P. & Diatroptov, M.E. Influence of Melatonin on Maturation of Male Germ Cells. Bull Exp Biol Med 175, 781–784 (2023). https://doi.org/10.1007/s10517-023-05946-4
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-023-05946-4