We studied the reparative and antioxidant effects of Thymogen and its new structural analogues obtained by binding amino acid D-Ala to the N- or C-end of the peptide molecule in acute toxic hepatopathy. Intragastric administration of carbon tetrachloride for 5 days caused the development of fat degeneration of hepatocytes, a decrease in catalase activity, and an increase in malondialdehyde concentration. Administration of peptides suppressed oxidative peroxidation and stimulated reparative regeneration of hepatocytes; Thymogen analogues produced more pronounced hepatotropic and antioxidant effects than Thymogen. Inclusion of D-Ala enhanced the effect of Thymogen on the processes of regeneration in hepatocytes and the antioxidant effect under conditions of acute carbon tetrachloride hepatopathy. The highest efficiency was achieved when the amino acid was added to the C-end of the molecule.
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References
Register of Medicinal Products of Russia. Encyclopedia. Issue 30. Moscow, 2022. Russian.
Bulgakov SA. Hexapeptide dalargin in clinical gastroenterology: 30 years’’ of clinical application of the drug. Ross. Zh. Gastroenterol. Gepatol. Koloproktol. 2016;26(3):103-112. Russian.
Jia F, Wang J, Peng J, Zhao P, Kong Z, Wang K, Yan W, Wang R. D-amino acid substitution enhances the stability of antimicrobial peptide polybia-CP. Acta Biochim. Biophys. Sin (Shanghai). 2017;49(10):916-925. https://doi.org/10.1093/abbs/gmx091
Xi W, Hansmann UHE. The effect of retro-inverse D-amino acid Aβ-peptides on Aβ-fibril formation. J. Chem. Phys. 2019;150(9):095101. https://doi.org/10.1063/1.5082194
Smakhtin MYu, Bobyncev YaI, Koroshvili VT, Chulanova AA, Dolzhikova IN. Immunotropic, antioxidant and reparative effects of a new thymogen analogue modified with D-alanine. Chelovek Zdorov’e. 2019;(1):77-82. Russian. https://doi.org/10.21626/vestnik/2019-1/09
Xanthopoulos A, Starling RC, Kitai T, Triposkiadis F. Heart failure and liver disease: cardiohepatic interactions. JACC Heart Fail. 2019;7(2):87-97. https://doi.org/10.1016/j.jchf.2018.10.007
Ginès P, Krag A, Abraldes JG, Solà E, Fabrellas N, Kamath PS. Liver cirrhosis. Lancet. 2021;398:1359-1376. https://doi.org/10.1016/S0140-6736(21)01374-X
McGill MR, Jaeschke H. Animal models of drug-induced liver injury. Biochim. Biophys. Acta Mol. Basis Dis. 2019;1865(5):1031-1039. https://doi.org/10.1016/j.bbadis.2018.08.037
Manual for Preclinical Studies of New Pharmacological Substances. Part I, Mironov AN, ed. Moscow, 2012. Russian.
Fomenko EV, Bobyntsev II, Kryukov AA, Ivanov AV, Andreeva LA, Myasoedov NF. Effect of Selank on functional state of rat hepatocytes under conditions of restraint stress. Bull. Exp. Biol. Med. 2017;163(4):415-418. https://doi.org/10.1007/s10517-017-3817-8
Iwata Y, Nakade Y, Kitajima S, Yoneda-Nakagawa S, Oshima M, Sakai N, Ogura H, Sato K, Toyama T, Yamamura Y, Miyagawa T, Yamazaki H, Hara A, Shimizu M, Furuichi K, Mita M, Hamase K, Tanaka T, Nishida M, Muramatsu W, Yamamoto H, Shichino S, Ueha S, Matsushima K, Wada T. Protective effect of d-alanine against acute kidney injury. Am. J. Physiol. Renal Physiol. 2022;322(6):F667-F679. https://doi.org/10.1152/ajprenal.00198.2021
Shi Y, Hussain Z, Zhao Y. Promising application of D-amino acids toward clinical therapy. Int. J. Mol. Sci. 2022;23(18):10794. https://doi.org/10.3390/ijms231810794
Avolio F, Martinotti S, Khavinson VK, Esposito JE Giambuzzi G, Marino A, Mironova E, Pulcini R, Robuffo I, Bologna G, Simeone P, Lanuti P, Guarnieri S, Trofimova S, Procopio AD, Toniato E. Peptides regulating proliferative activity and inflammatory pathways in the monocyte/macrophage THP-1 cell line. Int. J. Mol. Sci. 2022;23(7):3607. https://doi.org/10.3390/ijms23073607.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 175, No. 5, pp. 646-649, May, 2023
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Chulanova, A.A., Smakhtin, M.Y., Bobyntsev, I.I. et al. Reparative and Antioxidant Effects of New Analogues of Immunomodulator Thymogen in Experimental Model of Liver Damage. Bull Exp Biol Med 175, 700–703 (2023). https://doi.org/10.1007/s10517-023-05929-5
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DOI: https://doi.org/10.1007/s10517-023-05929-5