Sequestosome-1 (SQSTM1/p62) is one of the most important multifunctional proteins, which is necessary to maintain mitochondrial stability by eliminating damaged mitochondria through mitophagy. We studied the influence of age and diet on the expression of the p62 gene in the femoral and abdominal muscles of rats, as well as the integrity of some mitochondrial components. In the femoral muscles of 24-month-old rats receiving restricted ration, the expression of the p62 gene increased. We assume that activation of mitophagy contributed to a decrease in the levels of oxidative damage to mitochondrial DNA and LPO intensity in the femoral muscles of 24-month-old rats.
Similar content being viewed by others
References
Barbosa MC, Grosso RA, Fader CM. Hallmarks of aging: an autophagic perspective. Front. Endocrinol. (Lausanne). 2019;9:790. https://doi.org/10.3389/fendo.2018.00790
Zuo Z, Jing K, Wu H, Wang S, Ye L, Li Z, Yang C, Pan Q, Liu WJ, Liu HF. Mechanisms and functions of mitophagy and potential roles in renal disease. Front. Physiol. 2020;11:935. https://doi.org/10.3389/fphys.2020.00935
Kumar AV, Mills J, Lapierre LR. Selective autophagy receptor p62/SQSTM1, a pivotal player in stress and aging. Front. Cell Dev. Biol. 2022;10:793328. https://doi.org/10.3389/fcell.2022.793328
Gureev AP, Andrianova NV, Pevzner IB, Zorova LD, Chernyshova EV, Sadovnikova IS, Chistyakov DV, Popkov VA, Semenovich DS, Babenko VA, Silachev DN, Zorov DB, Plotnikov EY, Popov VN. Dietary restriction modulates mitochondrial DNA damage and oxylipin profile in aged rats. FEBS J. 2022;289(18):5697-5713. https://doi.org/10.1111/febs.16451
Salazar G, Cullen A, Huang J, Zhao Y, Serino A, Hilenski L, Patrushev N, Forouzandeh F, Hwang HS. SQSTM1/p62 and PPARGC1A/PGC-1alpha at the interface of autophagy and vascular senescence. Autophagy. 2020;16(6):1092-1110. https://doi.org/10.1080/15548627.2019.1659612
Yuan X, Tian GG, Pei X, Hu X, Wu J. Spermidine induces cytoprotective autophagy of female germline stem cells in vitro and ameliorates aging caused by oxidative stress through upregulated sequestosome-1/p62 expression. Cell Biosci. 2021;11(1):107. https://doi.org/10.1186/s13578-021-00614-4.
Pifferi F, Terrien J, Marchal J, Dal-Pan A, Djelti F, Hardy I, Chahory S, Cordonnier N, Desquilbet L, Hurion M, Zahariev A, Chery I, Zizzari P, Perret M, Epelbaum J, Blanc S, Picq JL, Dhenain M, Aujard F. Caloric restriction increases lifespan but affects brain integrity in grey mouse lemur primates. Commun. Biol. 2018;1:30. https://doi.org/10.1038/s42003-018-0024-8
Gutiérrez-Casado E, Khraiwesh H, López-Domínguez JA, Montero-Guisado J, López-Lluch G, Navas P, de Cabo R, Ramsey JJ, González-Reyes JA, Villalba JM. The impact of aging, calorie restriction and dietary fat on autophagy markers and mitochondrial ultrastructure and dynamics in mouse skeletal muscle. J. Gerontol. A Biol. Sci. Med. Sci. 2019;74(6):760-769. https://doi.org/10.1093/gerona/gly161
Bujak AL, Crane JD, Lally JS, Ford RJ, Kang SJ, Rebalka IA, Green AE, Kemp BE, Hawke TJ, Schertzer JD, Steinberg GR. AMPK activation of muscle autophagy prevents fasting-induced hypoglycemia and myopathy during aging. Cell Metab. 2015;21(6):883-890. https://doi.org/10.1016/j.cmet.2015.05.016
Pinto M, Moraes CT. Mechanisms linking mtDNA damage and aging. Free Radic. Biol. Med. 2015;85:250-258. https://doi.org/10.1016/j.freeradbiomed.2015.05.005
Dautant A, Meier T, Hahn A, Tribouillard-Tanvier D, di Rago JP, Kucharczyk R. ATP synthase diseases of mitochondrial genetic origin. Front. Physiol. 2018;9:329. https://doi.org/10.3389/fphys.2018.00329
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 175, No. 2, pp. 210-214, February, 2023
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Chernyshova, E.V., Gureev, A.P., Sadovnikova, I.S. et al. The Relationship of p62 Gene Expression with Integrity of Mitochondrial DNA and the Level of Lipid Peroxidation Products in Skeletal Muscles of Rats of Different Ages Exposed to Different Feeding Protocols. Bull Exp Biol Med 175, 245–248 (2023). https://doi.org/10.1007/s10517-023-05843-w
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-023-05843-w