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The Search for Single-Domain Antibodies Interacting with the Receptor-Binding Domain of SARS-CoV-2 Surface Protein

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We performed a search for nanoantibodies that specifically interact with the receptor-binding domain (RBD) of the SARS-CoV-2 surface protein. The specificity of single-domain antibodies from the blood sera of a llama immunized with RBD of SARS-CoV-2 surface protein S (variant B.1.1.7 (Alpha)) was analyzed by ELISA. Recombinant trimers of the SARS-CoV-2 spike protein were used as antigens. In this work, a set of single-domain antibodies was obtained that specifically bind to the RBD of the SARS-CoV-2 virus.

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References

  1. Maass DR, Sepulveda J, Pernthaner A, Shoemaker CB. Alpaca (Lama pacos) as a convenient source of recombinant camelid heavy chain antibodies (VHHs). J. Immunol. Methods. 2007;324(1-2):13-25. https://doi.org/10.1016/j.jim.2007.04.008

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Gorchakov AA, Kulemzin SV, Guselnikov SV, Baranov KO, Belovezhets TN, Mechetina LV, Volkova OY, Najakshin AM, Chikaev NA, Chikaev AN, Solodkov PP, Larichev VF, Gulyaeva MA, Markhaev AG, Kononova YV, Alekseyev AY, Shestopalov AM, Yusubalieva GM, Klypa TV, Ivanov AV, Valuev-Elliston VT, Baklaushev VP, Taranin AV. Isolation of a panel of ultra-potent human antibodies neutralizing SARS-CoV-2 and viral variants of concern. Cell Discov. 2021;7(1):96. https://doi.org/10.1038/s41421-021-00340-8

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Van Bockstaele F, Holz JB, Revets H. The development of nanobodies for therapeutic applications. Curr. Opin. Investig Drugs. 2009;10(11):1212-1224.

    PubMed  Google Scholar 

  4. Esmagambetov IB, Shcheblyakov DV, Egorova DA, Voronina OL, Derkaev AA, Voronina DV, Popova OA, Ryabova EI, Shcherbinin DN, Aksenova EI, Semenov AN, Kunda MS, Ryzhova NN, Zubkova OV, Tukhvatulin AI, Logunov DYu, Naroditsky BS, Borisevich SV, Gintsburg AL. Nanobodies are potential therapeutic agents for the Ebola virus infection. Acta Naturae. 2021;13(4):53-63. https://doi.org/10.32607/actanaturae.11487

  5. Detalle L, Stohr T, Palomo C, Piedra PA, Gilbert BE, Mas V, Millar A, Power UF, Stortelers C, Allosery K, Melero JA, Depla E. Generation and characterization of ALX-0171, a potent novel therapeutic nanobody for the treatment of respiratory syncytial virus infection. Antimicrob. Agents Chemother. 2015;60(1):6-13. https://doi.org/10.1128/AAC.01802-15

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Lu Q, Zhang Z, Li H, Zhong K, Zhao Q, Wang Z, Wu Z, Yang D, Sun S, Yang N, Zheng M, Chen Q, Long C, Guo W, Yang H, Nie C, Tong A. Development of multivalent nanobodies blocking SARS-CoV-2 infection by targeting RBD of spike protein. J. Nanobiotechnology. 2021;19(1):33. https://doi.org/10.1186/s12951-021-00768-w

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Zebardast A, Hosseini P, Hasanzadeh A, Latifi T. The role of single-domain antibodies (or nanobodies) in SARS-CoV-2 neutralization. Mol. Biol. Rep. 2022;49(1):647-656. https://doi.org/10.1007/s11033-021-06819-7

    Article  CAS  PubMed  Google Scholar 

  8. Tikunova NV, Morozova VV. Phage display on the base of filamentous bacteriophages: application for recombinant antibodies selection. Acta Naturae. 2009;1(3):20-28.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Vyatchanin AS, Tillib SV. Modifications in the phage display procedure to increase selection efficiency of antigen-binding domains on distinguished single-chain camel antibodies. Biotech. Russ. 2008;(4):30-38.

    Google Scholar 

  10. Brissette R, Goldstein NI. The use of phage display peptide libraries for basic and translational research. Methods Mol. Biol. 2007;383:203-213. https://doi.org/10.1007/978-1-59745-335-6_13

    Article  CAS  PubMed  Google Scholar 

  11. Pardon E, Laeremans T, Triest S, Rasmussen SG, Wohlkönig A, Ruf A, Muyldermans S, Hol WG, Kobilka BK, Steyaert J. A general protocol for the generation of Nanobodies for structural biology. Nat. Protoc. 2014;9(3):674-693. https://doi.org/10.1038/nprot.2014.039

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. World Health Organization (WHO). Coronavirus disease (COVID-19). URL: https://www.who.int/ru/emergencies/diseases/novel-coronavirus-2019

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Authors and Affiliations

  1. State Research Center of Virology and Biotechnology “VECTOR”, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia

    V. S. Aripov, N. V. Volkova, V. S. Nesmeyanova, A. A. Isaeva, Yu. A. Merkul’eva, D. V. Shanshin, S. V. Belenkaya, A. A. Ilyichev & D. N. Shcherbakov

  2. Institute of Molecular and Cellular Biology, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia

    A. V. Taranin, L. V. Mechetina, N. A. Chikaev & A. M. Nayakshin

Authors
  1. V. S. Aripov
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  2. N. V. Volkova
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  3. A. V. Taranin
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  4. L. V. Mechetina
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  5. N. A. Chikaev
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  6. A. M. Nayakshin
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  7. V. S. Nesmeyanova
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  8. A. A. Isaeva
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  9. Yu. A. Merkul’eva
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  10. D. V. Shanshin
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  11. S. V. Belenkaya
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  12. A. A. Ilyichev
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  13. D. N. Shcherbakov
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Corresponding author

Correspondence to V. S. Aripov.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 175, No. 2, pp. 188-191, February, 2023

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Aripov, V.S., Volkova, N.V., Taranin, A.V. et al. The Search for Single-Domain Antibodies Interacting with the Receptor-Binding Domain of SARS-CoV-2 Surface Protein. Bull Exp Biol Med 175, 225–228 (2023). https://doi.org/10.1007/s10517-023-05839-6

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  • DOI: https://doi.org/10.1007/s10517-023-05839-6

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