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Interaction of Graphene Oxide Nanoparticles with Human Mononuclear Cells in the Cell-IQ System

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The interaction of graphene oxide nanoparticles with human peripheral blood mononuclear cells was studied using the Cell-IQ continuous monitoring system for living cells. We used graphene oxide nanoparticles of various sizes coated with linear or branched polyethylene glycol (PEG) in concentrations of 5 and 25 μg/ml. After 24-h incubation with graphene oxide nanoparticles, the increase in the number of peripheral blood mononuclear cells at visualization points decreased; nanoparticles coated with branched PEG more markedly suppressed cell growth in culture. In the presence of graphene oxide nanoparticles, peripheral blood mononuclear cells retained high viability in culture after daily monitoring in the Cell-IQ system. The studied nanoparticles were engulfed by monocytes and the type of PEGylation had no effect on this process. Thus, graphene oxide nanoparticles reduced the increase in peripheral blood mononuclear cell mass during dynamic observation in the Cell-IQ system without reducing their viability.

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Authors and Affiliations

  1. Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences — Branch of Perm State Research Center, Ural Division of the Russian Academy of Sciences, Perm, Russia

    S. V. Uzhviyuk, P. V. Khramtsov, M. B. Raev, V. P. Timganova, M. S. Bochkova, O. G. Khaziakhmatova & S. A. Zamorina

  2. I. Kant Baltic Federal University, Kaliningrad, Russia

    O. G. Khaziakhmatova, V. V. Malashchenko & L. S. Litvinova

Authors
  1. S. V. Uzhviyuk
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  2. P. V. Khramtsov
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  3. M. B. Raev
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  4. V. P. Timganova
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  5. M. S. Bochkova
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  6. O. G. Khaziakhmatova
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  7. V. V. Malashchenko
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  8. L. S. Litvinova
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  9. S. A. Zamorina
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Correspondence to S. V. Uzhviyuk.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 62-68, March, 2023

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Uzhviyuk, S.V., Khramtsov, P.V., Raev, M.B. et al. Interaction of Graphene Oxide Nanoparticles with Human Mononuclear Cells in the Cell-IQ System. Bull Exp Biol Med 175, 172–178 (2023). https://doi.org/10.1007/s10517-023-05830-1

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