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The RNA-Binding Proteins OAS1, ZFP36L2, and DHX58 Are Involved in the Regulation of CD44 mRNA Splicing in Colorectal Cancer Cells

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Bulletin of Experimental Biology and Medicine Aims and scope

Regulation of alternative splicing is carried out by RNA-binding proteins. Each alternative splicing event is controlled by several RNA-binding proteins, which in combination create the distribution of alternative splicing products in a given cell type. Transmembrane protein CD44 plays an important role at various stages of the metastatic cascade and is considered as a promising molecule for the therapy of tumor diseases and the construction of prognostic classifiers. However, the functions of specific isoforms of this protein may differ significantly. In this work, we performed a bioinformatic search of RNA-binding proteins that can determine the expression of clinically significant isoforms 3 and 4 of CD44 protein. The analysis revealed five RNA-binding proteins, three of which (OAS1, ZFP36L2, and DHX58) are shown for the first time as potential regulators of the studied process.

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Authors and Affiliations

  1. Faculty of Biology and Biotechnologies, National Research University Higher School of Economics (HSE University), Moscow, Russia

    V. O. Novosad & D. V. Maltseva

  2. M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

    V. O. Novosad & D. V. Maltseva

Authors
  1. V. O. Novosad
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  2. D. V. Maltseva
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Correspondence to D. V. Maltseva.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 34-39, March, 2023

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Novosad, V.O., Maltseva, D.V. The RNA-Binding Proteins OAS1, ZFP36L2, and DHX58 Are Involved in the Regulation of CD44 mRNA Splicing in Colorectal Cancer Cells. Bull Exp Biol Med 175, 144–149 (2023). https://doi.org/10.1007/s10517-023-05826-x

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  • DOI: https://doi.org/10.1007/s10517-023-05826-x

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