Skip to main content

Advertisement

SpringerLink
Log in

Study of the Morphogenesis of Granulomas in the Liver of Mice in Different Age Periods Treated with Oxidized Dextran

  • MORPHOLOGY AND PATHOMORPHOLOGY
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

We studied granuloma formation and its outcomes in BCG-induced granulomatosis in the liver of mice of different age periods treated with oxidized dextran. Newborn C57BL/6 mice were intraperitoneally injected with BCG vaccine on the first day of life (group 1) or BCG vaccine solution on first day of life and oxidized dextran on the second day of life (group 2). Analysis was carried out on 3, 5, 10, 28, and 56 days of life. After injection of BCG vaccine, granulomas in the liver appeared starting from the day 28. In mice treated with oxidized dextran, granulomas on day 28 were smaller and less numerous than in group 1 animals. In BCG granulomatosis, fibroplastic processes in the liver develop mainly at the site of granulomas. Injection of oxidized dextran under conditions of BCG granulomatosis reduced the manifestations of fibrosis in the liver.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Global Tuberculosis Report 2021. Geneva: World Health Organization, 2021.

  2. Nadeev AP, Shkurupy VA, Marinkin IO. Liver and Placenta in Peri-and the Post-Natal Periods in Pathology. Novosibirsk, 2014. Russian.

  3. Peng W, Yang J, Liu E. Analysis of 170 cases of congenital TB reported in the literature between 1946 and 2009. Pediatr. Pulmonol. 2011;46(12):1215-1224. doi: https://doi.org/10.1002/ppul.21490

    Article  PubMed  Google Scholar 

  4. Vanden Driessche K, Persson A, Marais BJ, Fink PJ, Urdahl KB. Immune vulnerability of infants to tuberculosis. Clin. Dev. Immunol. 2013;2013:781320. doi: https://doi.org/10.1155/2013/781320

  5. Ravimohan S, Kornfeld H, Weissman D, Bisson GP. Tuberculosis and lung damage: from epidemiology to pathophysiology. Eur. Respir. Rev. 2018;27(147):170077. doi: https://doi.org/10.1183/16000617.0077-2017

  6. Mansukhani S, Shah I. Hepatic dysfunction in children with tuberculosis on treatment with antituberculous therapy. Ann. Hepatol. 2012;11(1):96-99.

    Article  CAS  PubMed  Google Scholar 

  7. Shkurupy VA. Tuberculous Granulomatosis. Cytophysiology and Targeted Therapy. Moscow, 2007. Russian.

  8. Tsafaras GP, Ntontsi P, Xanthou G. Advantages and limitations of the neonatal immune system. Front. Pediatr. 2020;8:5. doi: https://doi.org/10.3389/fped.2020.00005

    Article  PubMed  PubMed Central  Google Scholar 

  9. Kraft JD, Horzempa J, Davis C, Jung JY, Peña MM, Robinson CM. Neonatal macrophages express elevated levels of interleukin-27 that oppose immune responses. Immunology. 2013;139(4):484-493. doi: https://doi.org/10.1111/imm.12095

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Nakagaki BN, Mafra K, de Carvalho É, Lopes ME, Carvalho-Gontijo R, de Castro-Oliveira HM, Campolina-Silva GH, de Miranda CDM, Antunes MM, Silva ACC, Diniz AB, Alvarenga DM, Lopes MAF, de Souza Lacerda VA, Mattos MS, Araújo AM, Vidigal PVT, Lima CX, Mahecha GAB, Madeira MFM, Fernandes GR, Nogueira RF, Moreira TG, David BA, Rezende RM, Menezes GB. Immune and metabolic shifts during neonatal development reprogram liver identity and function. J. Hepatol. 2018;69(6):1294-1307. doi: https://doi.org/10.1016/j.jhep.2018.08.018

    Article  CAS  PubMed  Google Scholar 

  11. Upadhyay S, Mittal E, Philips JA. Tuberculosis and the art of macrophage manipulation. Pathog. Dis. 2018;76(4):fty037. doi: https://doi.org/10.1093/femspd/fty037

  12. Neshchadim DV, Arkhipov SA, Shkurupiy VA, Akhramenko ES, Troitskiy AV. Study of the effect of liposomal form of dextrazid on the expression of cathepsin B and D macrophages in vitro. Sib. Nauch. Med. Zh. 2017;37(6):16-21. Russian.

  13. Il’in DA, Shkurupy VA. The in vitro M1/M2 polarization of macrophages of BCG-infected mice. Bull. Exp. Biol. Med. 2020;169(4):467-469. doi: https://doi.org/10.1007/s10517-020-04910-w

  14. DiFazio RM, Mattila JT, Klein EC, Cirrincione LR, Howard M, Wong EA, Flynn JL. Active transforming growth factor-β is associated with phenotypic changes in granulomas after drug treatment in pulmonary tuberculosis. Fibrogenesis Tissue Repair. 2016;9:6. doi: https://doi.org/10.1186/s13069-016-0043-3

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Kozhin PM, Chechushkov AV, Zaytseva NS, Khrapova MV, Cherdantseva LA, Menshchikova EB, Troitsky AV, Shkurupy VA. Expression of protein genes participating in fibroplastic processes in mice lung during the development of tuberculous inflammation. Sib. Nauch. Med. Zh. 2019;39(4):22-29. Russian. doi: https://doi.org/10.15372/SSMJ20190403

Download references

Author information

Authors and Affiliations

  1. Novosibirsk State Medical University, Ministry of Health of the Russian Federation, Novosibirsk, Russia

    A. P. Nadeev, A. A. Abyshev & I. O. Marinkin

Authors
  1. A. P. Nadeev
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. A. Abyshev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. O. Marinkin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. P. Nadeev.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 175, No. 1, pp. 112-116, January, 2023

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Nadeev, A.P., Abyshev, A.A. & Marinkin, I.O. Study of the Morphogenesis of Granulomas in the Liver of Mice in Different Age Periods Treated with Oxidized Dextran. Bull Exp Biol Med 175, 96–100 (2023). https://doi.org/10.1007/s10517-023-05818-x

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-023-05818-x

Keywords

Search

Navigation