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Attenuated Strain of Mycobacterium tuberculosis BN: Characteristics

  • IMMUNOLOGY AND MICROBIOLOGY
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Bulletin of Experimental Biology and Medicine Aims and scope

We evaluated the vaccine properties of a novel attenuated strain of M. tuberculosis BN (Mtb BN) and its impact on the gut microbiota in inbred female mice in comparison with a virulent strain Mtb H37Rv and a vaccine strain BCG. The Mtb BN strain demonstrated the highest anti-tuberculosis vaccine effect in I/St mice highly susceptible to tuberculosis infection and the same effect as BCG in mice of the recombinant strain B6.I-100 and in β2 microglobulin gene knockout mice. No adverse effects of the new Mtb BN strain on the gut microbiota of BALB/c mice were revealed. The virulent strain Mtb H37Rv and the vaccine strain BCG decreased the main indicators of normocenosis (Bifidobacterium spp., Bifidobacterium animalis subsp. lactis, Akkermansia, and Erysipelotrichaceae) and led to disappearance of Clostridium perfingens, E. coli, Pseudomonas spp., which contributed to reduction of species diversity and the development of dysbiosis.

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Authors and Affiliations

  1. Central Research Institute of Tuberculosis, Moscow, Russia

    B. V. Nikonenko, N. N. Logunova, N. V. Sterzhanova & S. I. Kayukova

  2. V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia

    A. E. Donnikov

  3. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia

    S. P. Shchelykalina

Authors
  1. B. V. Nikonenko
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  2. A. E. Donnikov
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  3. N. N. Logunova
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  4. N. V. Sterzhanova
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  5. S. P. Shchelykalina
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  6. S. I. Kayukova
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Correspondence to B. V. Nikonenko.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 174, No. 9, pp. 329-342, September, 2022

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Nikonenko, B.V., Donnikov, A.E., Logunova, N.N. et al. Attenuated Strain of Mycobacterium tuberculosis BN: Characteristics. Bull Exp Biol Med 174, 341–345 (2023). https://doi.org/10.1007/s10517-023-05705-5

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  • DOI: https://doi.org/10.1007/s10517-023-05705-5

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