We studied the inhibitory effect of cyclooxygenase-2 inhibitor parecoxib on LPS-induced activation of BV2 microglia cells. The optimal dose of parecoxib (80 μmol/liter) was evaluated by the Cell Counting Kit-8. The cells were divided into the following groups: control (intact cells without treatment); LPS (treatment with 1 μg/ml LPS for 6 h), and experimental (pretreatment with 80 μmol/liter parecoxib for 24 h followed by incubation with 1 μg/ml LPS for 6 h). Cell morphology and proliferation and the expression of NLRP3, caspase-1, pro-caspase-1, and IL-1β were assessed. LPS induced significant morphological changes and decreased proliferation of primary BV2 cells in comparison with the control. These changes were prevented by parecoxib pretreatment. LPS significantly increased NLRP3 inflammatory vesicle activation and expression of NLRP3, caspase-1, pro-caspase-1, and IL-1β in comparison with the control group; pretreatment with parecoxib prevented all these changes. Our results suggest that pretreatment with parecoxib inhibited LPS-induced activation of BV2 microglial cells and probably inhibited NLRP3 inflammasome activation.
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08 January 2024
A Correction to this paper has been published: https://doi.org/10.1007/s10517-024-06012-3
References
Arora SS, Gooch JL, García PS. Postoperative cognitive dysfunction, Alzheimer’s disease, and anesthesia. Int. J. Neurosci. 2014;124(4):236-242. doi: https://doi.org/10.3109/00207454.2013.833919
Jurgens HA, Amancherla K, Johnson RW. Influenza infection induces neuroinflammation, alters hippocampal neuron morphology, and impairs cognition in adult mice. J. Neurosci. 2012;32(12):3958-3968. doi: https://doi.org/10.1523/JNEUROSCI.6389-11.2012
Montgomery SL, Bowers WJ. Tumor necrosis factor-alpha and the roles it plays in homeostatic and degenerative processes within the central nervous system. J. Neuroimmune Pharmacol. 2012;7(1):42-59. doi: https://doi.org/10.1007/s11481-011-9287-2
Yang SJ, Shao GF, Chen JL, Gong J. The NLRP3 Inflammasome: An Important Driver of Neuroinflammation in Hemorrhagic Stroke. Cell. Mol. Neurobiol. 2018;38(3):595-603. doi: https://doi.org/10.1007/s10571-017-0526-9
Bian YY, Wang LC, Qian WW, Lin J, Jin J, Peng HM, Weng XS. Role of Parecoxib Sodium in the Multimodal Analgesia after Total Knee Arthroplasty: A Randomized Double-blinded Controlled Trial. Orthop. Surg. 2018;10(4):321-327. doi: https://doi.org/10.1111/os.12410
Fielder E, Tweedy C, Wilson C, Oakley F, LeBeau FEN, Passos JF, Mann DA, von Zglinicki T, Jurk D. Anti-inflammatory treatment rescues memory deficits during aging in nfkb1-/- mice. Aging Cell. 2020;19(10):e13188. doi: https://doi.org/10.1111/acel.13188
Cudaback E, Jorstad NL, Yang Y, Montine TJ, Keene CD. Therapeutic implications of the prostaglandin pathway in Alzheimer’s disease. Biochem. Pharmacol. 2014;88(4):565-572. doi: https://doi.org/10.1016/j.bcp.2013.12.014
Yagami T, Koma H, Yamamoto Y. Pathophysiological Roles of Cyclooxygenases and Prostaglandins in the Central Nervous System. Mol. Neurobiol. 2016;53(7):4754-4771. doi: https://doi.org/10.1007/s12035-015-9355-3
Kolos EA, Korzhevskii DE. Activation of Microglyocytes in the Anterior Horns of Rat Spinal Cord after Administration of Bacterial Lipopolysaccharide. Bull. Exp. Biol. Med. 2017;163(4):515-518. doi: https://doi.org/10.1007/s10517-017-3841-8
Subhramanyam CS, Wang C, Hu Q, Dheen ST. Microglia-mediated neuroinflammation in neurodegenerative diseases. Semin. Cell. Dev. Biol. 2019;94:112-120. doi: https://doi.org/10.1016/j.semcdb.2019.05.004
Hoogland IC, Houbolt C, van Westerloo DJ, van Gool WA, van de Beek D. Systemic inflammation and microglial activation: systematic review of animal experiments. J. Neuroinflammation. 2015;12:114. doi: https://doi.org/10.1186/s12974-015-0332-6
Liu D, Zeng X, Li X, Cui C, Hou R, Guo Z, Mehta JL, Wang X. Advances in the molecular mechanisms of NLRP3 inflammasome activators and inactivators. Biochem. Pharmacol. 2020;175:113863. doi: https://doi.org/10.1016/j.bcp.2020.113863
Heneka MT, McManus RM, Latz E. Inflammasome signalling in brain function and neurodegenerative disease. Nat. Rev. Neurosci. 2018;19(10):610-621. doi: https://doi.org/10.1038/s41583-018-0055-7
Yang HH, Duan JX, Liu SK, Xiong JB, Guan XX, Zhong WJ, Sun CC, Zhang CY, Luo XQ, Zhang YF, Chen P, Hammock BD, Hwang SH, Jiang JX, Zhou Y, Guan CX. A COX-2/sEH dual inhibitor PTUPB alleviates lipopolysaccharide-induced acute lung injury in mice by inhibiting NLRP3 inflammasome activation. Theranostics. 2020;10(11):4749-4761. doi: https://doi.org/10.7150/thno.43108
Hua KF, Chou JC, Ka SM, Tasi YL, Chen A, Wu SH, Chiu HW, Wong WT, Wang YF, Tsai CL, Ho CL, Lin CH. Cyclooxygenase-2 regulates NLRP3 inflammasome-derived IL-1β production. J. Cell. Physiol. 2015;230(4):863-874. doi: https://doi.org/10.1002/jcp.24815
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 174, No. 8, pp. 172-178, July, 2022
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Li, X., Zhou, J.X., Qu, Y.D. et al. Сyclooxygenase-2 Inhibitor Parecoxib Reduces LPS-Induced Activation of BV2 Microglia Cells. Bull Exp Biol Med 174, 210–215 (2022). https://doi.org/10.1007/s10517-023-05675-8
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DOI: https://doi.org/10.1007/s10517-023-05675-8