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Hypothyroidism Modeling in Wistar Rats: Comparison of Two Surgical Methods

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In this study we present 2 surgical models of hypothyroidism in male Wistar rats (n=80) based on total thyroidectomy. Animals weighing 180-200 g were randomly divided into sham-operated group and 2 experimental groups. Thyroidectomy was performed by 2 different methods: primary ligation of either thyroid artery (TE-I) or vein (TE-II). The success of the model was verified through general postoperative conditions, serum hormone levels, histological study, and neck ultrasound. Hypothyroidism was successfully reproduced in both TE-I and TE-II models. TE-I was characterized by lower intra- and post-operative mortality, while TE-II provided better surgical exposure to the key anatomical sites.

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References

  1. Hodes S. Investigating hypothyroidism: how to take levothyroxine. BMJ. 2021;373:n1463. https://doi.org/10.1136/bmj.n1463

  2. Gilbert ME, Hedge JM, Valentín-Blasini L, Blount BC, Kannan K, Tietge J, Zoeller RT, Crofton KM, Jarrett JM, Fisher JW. An animal model of marginal iodine deficiency during development: the thyroid axis and neurodevelopmental outcome. Toxicol. Sci. 2013;132(1):177-195. https://doi.org/10.1093/toxsci/kfs335

    Article  CAS  PubMed  Google Scholar 

  3. Tank JC, Weiner DS, Jacquet R, Childs D, Ritzman TF, Horne WI, Steiner R, Morscher MA, Landis WJ. The effects of hypothyroidism on the proximal femoral physis in miniature swine. J. Orthop. Res. 2013;31(12):1986-1991. https://doi.org/10.1002/jor.22467

    Article  CAS  PubMed  Google Scholar 

  4. D’Amours GH, Taylor SM, Olfert ED, Simko E, Allen AL. Evaluation of experimental methods to induce congenital hypothyroidism in guinea pigs for use in the study of congenital hypothyroidism in horses. Am. J. Vet. Res. 2004;65(9):1251-1258. https://doi.org/10.2460/ajvr.2004.65.1251

    Article  PubMed  Google Scholar 

  5. Wondisford FE. Thyroid hormone action: insight from transgenic mouse models. J. Investig. Med. 2003;51(4):215-220. https://doi.org/10.1136/jim-51-04-22

    Article  CAS  PubMed  Google Scholar 

  6. Sun J, Hui C, Xia T, Xu M, Deng D, Pan F, Wang Y. Effect of hypothyroidism on the hypothalamic-pituitary-ovarian axis and reproductive function of pregnant rats. BMC Endocr Disord. 2018;18(1):30. https://doi.org/10.1186/s12902-018-0258-y

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Löf C, Patyra K, Kero A, Kero J. Genetically modified mouse models to investigate thyroid development, function and growth. Best Pract. Res. Clin. Endocrinol. Metab. 2018;32(3):241-256. https://doi.org/10.1016/j.beem.2018.03.007

    Article  CAS  PubMed  Google Scholar 

  8. Shan L, Zhou Y, Peng S, Wang X, Shan Z, Teng W. Implantation failure in rats with subclinical hypothyroidism is associated with LIF/STAT3 signaling. Endocr. Connect. 2019;8(6):718-727. https://doi.org/10.1530/EC-19-0185

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Xue D, Sun JL, Yang J. Early L-T4 intervention improves fetal heart development in pregnant rats with subclinical hypothyroidism rats by activating BMP4/Smad4 signaling pathway. BMC Cardiovasc. Disord. 2020;20(1):369. https://doi.org/10.1186/s12872-020-01646-3

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Yuan H, Shi Y, Li Y, Yang L, Tang Y, Hu Z, Du R, Li X, Gu L, Wang H, Xi C, Tang C. Evaluation of a TPTX model induced by ischemia. Exp. Anim. 2017;66(4):345-356. https://doi.org/10.1538/expanim.17-0029

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Liu Y, Jin S, Yang YT, Bai Y, Yong H, Bao W. Cognitive dysfunction associated with activation of the mTOR signaling pathway after TSH suppression therapy in rats. Endocr. J. 2020;67(10):1063-1070. https://doi.org/10.1507/endocrj.EJ20-0134

    Article  PubMed  Google Scholar 

  12. Shao SS, Zhao YF, Song YF, Xu C, Yang JM, Xuan SM, Yan HL, Yu CX, Zhao M, Xu J, Zhao JJ. Dietary high-fat lard intake induces thyroid dysfunction and abnormal morphology in rats. Acta Pharmacol. Sin. 2014;35(11):1411-20. https://doi.org/10.1038/aps.2014.82

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Li J, Liu Z, Zhao H, Yun F, Liang Z, Wang D, Zhao X, Zhang J, Cang H, Zou Y, Li Y. Alterations in atrial ion channels and tissue structure promote atrial fibrillation in hypothyroid rats. Endocrine. 2019;65(2):338-347. https://doi.org/10.1007/s12020-019-01968-z

    Article  CAS  PubMed  Google Scholar 

  14. Baki AM, Aydın AF, Vural P, Olgaç V, Doğru Abbasoğlu S, Uysal M. α-Lipoic Acid Ameliorates The Changes in Prooxidant-Antioxidant Balance in Liver and Brain Tissues of Propylthiouracil-Induced Hypothyroid Rats. Cell. J. 2020;22(Suppl 1):117-124. https://doi.org/10.22074/cellj.2020.7049

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Authors and Affiliations

  1. Department of Thyroid Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China

    L. J. Xie, Q. You, F. Wu, R. Hai & X. Y. Zhou

  2. Department of Thyroid and Breast Surgery, The First People’s Hospital of Zigong, Zigong, Sichuan, China

    L. J. Xie

  3. Department of Anesthesiology, Ya’an People’s Hospital, Ya’an, Sichuan, China

    S. Z. Zhou

Authors
  1. L. J. Xie
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  2. Q. You
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  3. S. Z. Zhou
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  4. F. Wu
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  5. R. Hai
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  6. X. Y. Zhou
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Corresponding author

Correspondence to X. Y. Zhou.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 173, No. 6, pp. 788-795, June, 2022

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Xie, L.J., You, Q., Zhou, S.Z. et al. Hypothyroidism Modeling in Wistar Rats: Comparison of Two Surgical Methods. Bull Exp Biol Med 173, 794–800 (2022). https://doi.org/10.1007/s10517-022-05634-9

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  • DOI: https://doi.org/10.1007/s10517-022-05634-9

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