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Light-Induced Functional Pinealectomy: Expression of MT2 Receptors in Liver Cells of C57BL/6 Mice after Melatonin Treatment

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We performed an immunohistochemical study of MT2 melatonin receptor expression in the liver of C57BL/6 mice with modeled light-induced functional pinealectomy and after melatonin administration by the indirect avidin-biotin peroxidase ABC method. The animals were kept for 14 days under constant lighting. Intragastric administration of melatonin in physiological doses (1 mg/kg body weight for 14 days) to mice with light-induced functional pinealectomy resulted in a 2-fold increase in the relative expression area of MT2 receptors in liver cells in comparison with that in animals kept under standard lighting conditions, 24-h lighting for 14 days, or 24-h lighting receiving placebo (intragastric administration of 200 ml distilled water). Melatonin treatment had practically no effect on MT2 staining intensity. Our results attest to the important role of MT2 receptors in melatonin synthesis disorders and can serve as the basis for the development of therapeutic strategies aimed at melatonin receptors.

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References

  1. Michurina SV, Ishchenko IY, Arkhipov SA, Letyagin AY, Korolev MA, Zavjalov EL. The expression of apoptosis-regulating proteins Bcl-2 and Bad in liver cells of C57BL/6 mice under light-induced functional pinealectomy and after correction with MT. Vavilovskii Zhurnal Genet Selektsii. 2021;25(3):310-317.

    CAS  PubMed  PubMed Central  Google Scholar 

  2. Emet M, Ozcan H, Ozel L, Yayla M, Halici Z, Hacimuftuoglu A. A review of melatonin, its receptors and drugs. Eurasian J. Med. 2016;48(2):135-141. https://doi.org/10.5152/eurasianjmed.2015.0267

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Fonken LK, Nelson RJ. The effects of light at night on circadian clocks and metabolism. Endocr. Rev. 2014;35(4):648-670. https://doi.org/10.1210/er.2013-1051

    Article  CAS  PubMed  Google Scholar 

  4. Giudice A, Crispo A, Grimaldi M, Polo A, Bimonte S, Capunzo M, Amore A, D’Arena G, Cerino P, Budillon A, Botti G, Costantini S, Montella M. The Effect of Light Exposure at Night (LAN) on carcinogenesis via decreased nocturnal melatonin synthesis. Molecules. 2018;23(6):1308. https://doi.org/10.3390/molecules23061308

    Article  CAS  PubMed Central  Google Scholar 

  5. lHajam YA, Rai S. Melatonin and insulin modulates the cellular biochemistry, histoarchitecture and receptor expression during hepatic injury in diabetic rats.Life Sci. 2019;239:117046. https://doi.org/10.1016/j.lfs.2019.117046

  6. Hardeland R. Neurobiology, pathophysiology, and treatment of melatonin deficiency and dysfunction. ScientificWorldJournal. 2012;2012:640389. https://doi.org/10.1100/2012/640389

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Jilg A, Bechstein P, Saade A, Dick M, Li TX, Tosini G, Rami A, Zemmar A, Stehle JH. Melatonin modulates daytime-dependent synaptic plasticity and learning efficiency. J. Pineal Res. 2019;66(3):e12553. https://doi.org/10.1111/jpi.12553

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Jockers R, Delagrange P, Dubocovich ML, Markus RP, Renault N, Tosini G, Cecon E, Zlotos DP. Update on melatonin receptors: IUPHAR Review 20. Br. J. Pharmacol. 2016;173(18):2702-2725. https://doi.org/10.1111/bph.13536

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Laskar P, Singh SS. Melatonin receptor (MT1 and MT2) proteins expression in thyroid gland and level of thyroxin (T4), thyrotropin (TSH) hormone during progression of age in male SWISS albino mice. J. Biol. Sci. Opin. 2015;3(1):1-6. https://doi.org/10.7897/2321-6328.0311.

    Article  Google Scholar 

  10. Mathes AM, Heymann P, Ruf C, Huhn R, Hinkelbein J, Volk T, Fink T. Endogenous and exogenous melatonin exposure attenuates hepatic MT1 melatonin receptor protein expression in rat. Antioxidants (Basel). 2019;8(9):408. https://doi.org/10.3390/antiox8090408

    Article  CAS  PubMed Central  Google Scholar 

  11. Morin LP. Nocturnal light and nocturnal rodents: similar regulation of disparate functions? J. Biol. Rhythms. 2013;28(2):95-106. https://doi.org/10.1177/0748730413481921

    Article  CAS  PubMed  Google Scholar 

  12. Nagorny C, Lyssenko V. Tired of diabetes genetics? Circadian rhythms and diabetes: the MTNR1B story? Curr. Diab. Rep. 2012;12(6):667-672. https://doi.org/10.1007/s11892-012-0327-y

    Article  CAS  PubMed  Google Scholar 

  13. Pandi-Perumal SR, Trakht I, Srinivasan V, Spence DW, Maestroni GJ, Zisapel N, Cardinali DP. Physiological effects of melatonin: role of melatonin receptors and signal transduction pathways. Prog. Neurobiol. 2008;85(3):335-353. https://doi.org/10.1016/j.pneurobio.2008.04.001

    Article  CAS  PubMed  Google Scholar 

  14. Reiter RJ, Tan DX, Rosales-Corral S, Galano A, Zhou XJ, Xu B. Mitochondria: central organelles for melatonin’s antioxidant and anti-aging actions.Molecules. 2018;23(2):509. https://doi.org/10.3390/molecules23020509.

    Article  CAS  PubMed Central  Google Scholar 

  15. Renzi A, Glaser S, Demorrow S, Mancinelli R, Meng F, Franchitto A, Venter J, White M, Francis H, Han Y, Alvaro D, Gaudio E, Carpino G, Ueno Y, Onori P, Alpini G. Melatonin inhibits cholangiocyte hyperplasia in cholestatic rats by interaction with MT1 but not MT2 melatonin receptors. Am. J. Physiol. Gastrointest. Liver Physiol. 2011;301(4):G634-G643. https://doi.org/10.1152/ajpgi.00206.2011

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Sato K, Meng F, Francis H, Wu N, Chen L, Kennedy L, Zhou T, Franchitto A, Onori P, Gaudio E, Glaser S, Alpini G. Melatonin and circadian rhythms in liver diseases: Functional roles and potential therapies. J. Pineal Res. 2020;68(3):e12639. https://doi.org/10.1111/jpi.12639

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Sheen JM, Chen YC, Hsu MH, Tain YL, Huang YH, Tiao MM, Li SW, Huang LT. Melatonin alleviates liver apoptosis in bile duct ligation young rats. Int. J. Mol. Sci. 2016;17(8):1365. https://doi.org/10.3390/ijms17081365

    Article  CAS  PubMed Central  Google Scholar 

  18. Srinivasan V, Ohta Y, Espino J, Pariente JA, Rodriguez AB, Mohamed M, Zakaria R. Metabolic syndrome, its pathophysiology and the role of melatonin. Recent Pat Endocr. Metab. Immune Drug Discov. 2013;7(1):11-25.

    Article  CAS  Google Scholar 

  19. Turkowska E, Majewski PM, Rai S, Skwarlo-Sonta K. Pineal oscillator functioning in the chicken — effect of photoperiod and melatonin. Chronobiol. Int. 2014;31(1):134-143. https://doi.org/10.3109/07420528.2013.832279

    Article  CAS  PubMed  Google Scholar 

  20. Venegas C, García JA, Doerrier C, Volt H, Escames G, López LC, Reiter RJ, Acuña-Castroviejo D. Analysis of the daily changes of melatonin receptors in the rat liver. J. Pineal Res. 2013;54(3):313-321. https://doi.org/10.1111/jpi.12019

    Article  CAS  PubMed  Google Scholar 

  21. Zhang Z, Silveyra E, Jin N, Ribelayga CP. A congenic line of the C57BL/6J mouse strain that is proficient in melatonin synthesis. J. Pineal Res. 2018;65(3):e12509. https://doi.org/10.1111/jpi.12509

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Research Institute of Clinical and Experimental Lymphology — Affiliated Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia

    S. V. Michurina, I. Yu. Ishchenko & S. A. Arkhipov

  2. Scientific Center for Family Health and Human Reproduction Problems, Irkutsk, Russia

    S. I. Kolesnikov

  3. M. V. Lomonosov Moscow State University, Moscow, Russia

    S. I. Kolesnikov

Authors
  1. S. V. Michurina
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  2. S. I. Kolesnikov
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  3. I. Yu. Ishchenko
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  4. S. A. Arkhipov
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Correspondence to S. V. Michurina.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 2, pp. 127-132, June, 2022

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Michurina, S.V., Kolesnikov, S.I., Ishchenko, I.Y. et al. Light-Induced Functional Pinealectomy: Expression of MT2 Receptors in Liver Cells of C57BL/6 Mice after Melatonin Treatment. Bull Exp Biol Med 173, 569–574 (2022). https://doi.org/10.1007/s10517-022-05590-4

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  • DOI: https://doi.org/10.1007/s10517-022-05590-4

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