Skip to main content

Advertisement

SpringerLink
Log in

Differential Markers of Subpopulations of Epithelial Cells of the Larynx in Squamous Cell Carcinoma

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

In squamous cell carcinoma of the larynx, the population of epithelial cells in the tumor tissue is initially heterogeneous and, in addition to tumor cells invading the organ mucosa, includes normal epithelial cells of protein-mucous glands and cells of the stratified epithelium covering the mucous membrane. A search for differential markers to separate these subpopulations was carried out. The surface marker CD44 and cytokeratins 5 and 17 that are often used to verify carcinoma cells, are common markers for all epithelial cells of the larynx. In highly differentiated carcinoma, subpopulations of normal and tumor epithelial cells can be separated by the level of expression of cytokeratins 10 and 18 and nuclear markers Ki-67 and p63. However, in moderately differentiated carcinoma, tumor cells and normal cells of the basal layer of the stratified epithelium covering the mucous membrane of the larynx have similar phenotypes, which should be taken into account when conducting experimental studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ali AA, Al-Jandan BA, Suresh CS. The importance of ctokeratins in the early detection of oral squamous cell carcinoma. J. Oral Maxillofac. Pathol. 2018;22(3):441. https://doi.org/10.4103/jomfp.JOMFP_238_17

    Article  PubMed  PubMed Central  Google Scholar 

  2. Borba M, Cernea C, Dias F, Faria P, Bacchi C, Brandão L, Costa A. Expression profile of p63 in 127 patients with laryngeal squamous cell carcinoma. ORL J. Otorhinolaryngol. Relat. Spec. 2010;72(6):319-324. https://doi.org/10.1159/000319904

    Article  CAS  PubMed  Google Scholar 

  3. Canning M, Guo G, Yu M, Myint C, Groves MW, Byrd JK, Cui Y. Heterogeneity of the head and neck squamous cell carcinoma immune landscape and its impact on immunotherapy. Front. Cell Dev. Biol. 2019;7:52. https://doi.org/10.3389/fcell.2019.00052

    Article  PubMed  PubMed Central  Google Scholar 

  4. Caruntu A, Moraru L, Lupu M, Ciubotaru DA, Dumitrescu M, Eftimie L, Hertzog R, Zurac S, Caruntu C, Voinea OC. Assessment of histological features in squamous cell carcinoma involving head and neck skin and mucosa. J. Clin. Med. 2021;10(11):2343. https://doi.org/10.3390/jcm10112343

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Chatzkel J, Lewis JS Jr, Ley JC, Wildes TM, Thorstad W, Gay H, Daly M, Jackson R, Rich J, Paniello R, Nussenbaum B, Liu J, Siegel BA, Dehdashti F, Adkins D. Correlation of Ki-67 proliferative antigen expression and tumor response to induction chemotherapy containing cell cycle-specific agents in head and neck squamous cell carcinoma. Head Neck Pathol. 2017;11(3):338-345. https://doi.org/10.1007/s12105-016-0775-9

    Article  PubMed  Google Scholar 

  6. Chen J, Zhou J, Lu J, Xiong H, Shi X, Gong L. Significance of CD44 expression in head and neck cancer: a systemic review and meta-analysis. BMC Cancer. 2014;14:15. https://doi.org/10.1186/1471-2407-14-15

    Article  PubMed  PubMed Central  Google Scholar 

  7. Cirillo N, Wu C, Prime SS. Heterogeneity of cancer stem cells in tumorigenesis, metastasis, and resistance to antineoplastic treatment of head and neck tumours. Cells. 2021;10(11):3068. https://doi.org/10.3390/cells10113068

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Demers I, Donkers J, Kremer B, Speel EJ. Ex vivo culture models to indicate therapy response in head and neck squamous cell carcinoma. Cells. 2020;9(11):2527. https://doi.org/10.3390/cells9112527

    Article  CAS  PubMed Central  Google Scholar 

  9. Di Como CJ, Urist MJ, Babayan I, Drobnjak M, Hedvat CV, Teruya-Feldstein J, Pohar K, Hoos A, Cordon-Cardo C. p63 expression profiles in human normal and tumor tissues. Clin. Cancer Res. 2002;8(2):494-501.

    PubMed  Google Scholar 

  10. Fischer AH, Jacobson KA, Rose J, Zeller R. Preparation of Cells and Tissues for Fluorescence Microscopy. Basic Methods in Microscopy. Spector D, Goldman R, eds. New York, 2006. P. 103-123.

  11. Gunti S, Hoke ATK, Vu KP, London NR Jr. Organoid and spheroid tumor models: techniques and applications. Cancers (Basel). 2021;13(4):874. https://doi.org/10.3390/cancers13040874

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Johnson DE, Burtness B, Leemans CR, Lui VWY, Bauman JE, Grandis JR. Head and neck squamous cell carcinoma. Nat. Rev. Dis. Primers. 2020;6(1):92. https://doi.org/10.1038/s41572-020-00224-3

    Article  PubMed  PubMed Central  Google Scholar 

  13. Kumar V, Vashishta M, Kong L, Wu X, Lu JJ, Guha C, Dwarakanath BS. The Role of Notch, Hedgehog, and Wnt signaling pathways in the resistance of tumors to anticancer therapies. Front. Cell Dev. Biol. 2021;9:650772. https://doi.org/10.3389/fcell.2021.650772

    Article  PubMed  PubMed Central  Google Scholar 

  14. Kürten CHL, Kulkarni A, Cillo AR, Santos PM, Roble AK, Onkar S, Reeder C, Lang S, Chen X, Duvvuri U, Kim S, Liu A, Tabib T, Lafyatis R, Feng J, Gao SJ, Bruno TC, Vignali DAA, Lu X, Bao R, Vujanovic L, Ferris RL. Investigating immune and non-immune cell interactions in head and neck tumors by single-cell RNA sequencing. Nat. Commun. 2021;12(1):7338. https://doi.org/10.1038/s41467-021-27619-4

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. La Fleur L, Johansson AC, Roberg K. A CD44high/EGFRlow subpopulation within head and neck cancer cell lines shows an epithelial–mesenchymal transition phenotype and resistance to treatment. PLoS One. 2012;7(9):e44071. https://doi.org/10.1371/journal.pone.0044071

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Major AG, Pitty LP, Farah CS. Cancer stem cell markers in head and neck squamous cell carcinoma. Stem Cells Int. 2013;2013:319489. https://doi.org/10.1155/2013/319489

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Menz A, Weitbrecht T, Gorbokon N, Büscheck F, Luebke AM, Kluth M, Hube-Magg C, Hinsch A, Höflmayer D, Weidemann S, Fraune C, Möller K, Bernreuther C, Lebok P, Clauditz T, Sauter G, Uhlig R, Wilczak W, Steurer S, Minner S, Burandt E, Krech R, Dum D, Krech T, Marx A, Simon R. Diagnostic and prognostic impact of cytokeratin 18 expression in human tumors: a tissue microarray study on 11,952 tumors. Mol. Med. 2021;27(1):16. https://doi.org/10.1186/s10020-021-00274-7

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Mestrinho LA, Pissarra H, Faísca PB, Bragança M, Peleteiro MC, Niza MM. p63 and E-cadherin expression in canine oral squamous cell carcinoma. Vet. Pathol. 2015;52(4):614-620. https://doi.org/10.1177/0300985814547391

    Article  CAS  PubMed  Google Scholar 

  19. Sharaf K, Lechner A, Haider SP, Wiebringhaus R, Walz C, Kranz G, Canis M, Haubner F, Gires O, Baumeister P. Discrimination of cancer stem cell markers ALDH1A1, BCL11B, BMI-1, and CD44 in different tissues of HNSCC patients. Curr. Oncol. 2021;28(4):2763-2774. https://doi.org/10.3390/curroncol28040241

    Article  PubMed  PubMed Central  Google Scholar 

  20. Steurer S, Riemann C, Büscheck F, Luebke A.M, Kluth M, Hube-Magg C, Hinsch A, Höflmayer D, Weidemann S, Fraune C, Möller K, Menz A, Fisch M, Rink M, Bernreuther C, Lebok P, Clauditz TS, Sauter G, Uhlig R, Wilczak W, Dum D, Simon R, Minner S, Burandt E, Krech R, Krech T, Marx AH. p63 expression in human tumors and normal tissues: a tissue microarray study on 10,200 tumors. Biomark. Res. 2021;9(1):7. https://doi.org/10.1186/s40364-021-00260-5

  21. Svobodova M, Raudenska M, Gumulec J, Balvan J, Fojtu M, Kratochvilova M, Polanska H, Horakova Z, Kostrica R, Babula P, Heger Z, Masarik M. Establishment of oral squamous cell carcinoma cell line and magnetic bead-based isolation and characterization of its CD90/CD44 subpopulations. Oncotarget. 2017;8(39):66 254-66 269. https://doi.org/10.18632/oncotarget.19914

  22. Tanaka N, Osman AA, Takahashi Y, Lindemann A, Patel AA, Zhao M, Takahashi H, Myers JN. Head and neck cancer organoids established by modification of the CTOS method can be used to predict in vivo drug sensitivity.Oral Oncol. 2018;87:49-57. https://doi.org/10.1016/j.oraloncology.2018.10.018

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Vasca V, Vasca E, Freiman P, Marian D, Luce A, Mesolella M, Caraglia M, Ricciardiello F, Duminica T. Keratin 5 expression in squamocellular carcinoma of the head and neck. Oncol. Lett. 2014;8(6):2501-2504. https://doi.org/10.3892/ol.2014.2591

    Article  PubMed  PubMed Central  Google Scholar 

  24. WHO Report On Cancer: Setting Priorities, Investing Wisely and Providing Care for All. World Health Organization, 2020. URL: https://apps.who.int/iris/handle/10665/330745.

  25. Xu ES, Yang MH, Liu CY, Liu KW, Yang TT, Chou TY, Hwang TZ, Hsu CT. Decreasing cytokeratin 17 expression in head and neck cancer predicts nodal metastasis and poor prognosis: The first evidence. Clin. Otolaryngol. 2018;43(4):1010-1018. https://doi.org/10.1111/coa.13092

    Article  PubMed  Google Scholar 

  26. Yi CH, Jim Zhai Q, Wang BY. Updates on immunohistochemical and molecular markers in selected head and neck diagnostic problems. Arch. Pathol. Lab. Med. 2017;141(9):1214-1235. https://doi.org/10.5858/arpa.2016-0245-RA

    Article  PubMed  Google Scholar 

  27. Yoh K, Prywes R. Pathway regulation of p63, a director of epithelial cell fate. Front. Endocrinol. (Lausanne). 2015;6:51. https://doi.org/10.3389/fendo.2015.00051

Download references

Author information

Authors and Affiliations

  1. Peoples’ Friendship University of Russia (RUDN University), Moscow, Russia

    I. V. Arutyunyan, K. B. Gordon, A. V. Makarov, A. V. Lokhonina & T. Kh. Fatkhudinov

  2. V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia

    I. V. Arutyunyan

  3. A. P. Avtsyn Research Institute of Human Morphology, Moscow, Russia

    A. G. Soboleva, A. V. Makarov, A. V. Lokhonina & T. Kh. Fatkhudinov

  4. A. F. Tsyb Medical Radiological Research Center — Affiliated Branch of the National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Obninsk, Russia

    K. B. Gordon

  5. P. A. Hertsen Moscow Oncology Research Institute — Affiliated Branch of National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia

    D. S. Kudashkina, D. A. Miroshnichenko, A. P. Polyakov & I. V. Rebrikova

Authors
  1. I. V. Arutyunyan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. G. Soboleva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. B. Gordon
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. S. Kudashkina
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. D. A. Miroshnichenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. P. Polyakov
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. I. V. Rebrikova
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. A. V. Makarov
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. A. V. Lokhonina
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. T. Kh. Fatkhudinov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to I. V. Arutyunyan.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 2, pp. 109-116, June, 2022

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Arutyunyan, I.V., Soboleva, A.G., Gordon, K.B. et al. Differential Markers of Subpopulations of Epithelial Cells of the Larynx in Squamous Cell Carcinoma. Bull Exp Biol Med 173, 553–559 (2022). https://doi.org/10.1007/s10517-022-05588-y

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-022-05588-y

Key Words

Search

Navigation