Skip to main content
SpringerLink
Log in

Ultrastructural Immunocytochemistry of GABAergic Cells in Neocortical Neurotransplants

  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Neural transplantation is a promising regenerative therapy in the treatment of several neurological diseases. Importantly, transplanted tissue should not become a source of pathological functional activity. To assess the possibility of maintaining the balance between excitatory and inhibitory processes, an electron microscopic immunochemical study of the GABAergic system in rat neocortical transplants was performed. Accumulation of GABA-positive label in astrocytes and a relatively insignificant immune reaction to GABA in neurons and synaptic endings were found. These findings suggest that under conditions of impaired differentiation of GABA-containing neurons that generate phasic inhibition through inhibitory synapses, tonic inhibitory influences predominate in neurotransplants due to GABA released from astrocytes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Zhuravleva ZN, Hutsyan SS, Zhuravlev GI. Phenotypic differentiation of neurons in intraocular transplants. Rus. J. Dev. Biol. 2016;47(3):147-153. doi: https://doi.org/10.1134/S1062360416030085

    Article  CAS  Google Scholar 

  2. Buckmaster PS, Abrams E, Wen X. Seizure frequency correlates with loss of dentate gyrus GABAergic neurons in a mouse model of temporal lobe epilepsy. J. Comp. Neurol. 2017;525(11):2592-2610. doi: https://doi.org/10.1002/cne.24226

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Glykys J, Mody I. The main source of ambient GABA responsible for tonic inhibition in the mouse hippocampus. J. Physiol. 2007;582(Pt 3):1163-1178. doi: https://doi.org/10.1113/jphysiol.2007.134460

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Jo S, Yarishkin O, Hwang YJ, Chun YE, Park M, Woo DH, Bae JY, Kim T, Lee J, Chun H, Park HJ, Lee DY, Hong J, Kim HY, Oh SJ, Park SJ, Lee H, Yoon BE, Kim Y, Jeong Y, Shim I, Bae YC, Cho J, Kowall NW, Ryu H, Hwang E, Kim D, Lee CJ. GABA from reactive astrocytes impairs memory in mouse models of Alzheimer’s disease. Nat. Med. 2014;20(8):886-896. doi: https://doi.org/10.1038/nm.3639

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Kajita Y, Mushiake H. Heterogeneous GAD65 expression in subtypes of GABAergic neurons across layers of the cerebral cortex and hippocampus. Front. Behav. Neurosci. 2021;15:750869. doi: https://doi.org/10.3389/fnbeh.2021.750869

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Le Meur K, Mendizabal-Zubiaga J, Grandes P, Audinat E. GABA release by hippocampal astrocytes. Front. Comput. Neurosci. 2012;6:59. doi: https://doi.org/10.3389/fncom.2012.00059

    Article  PubMed  PubMed Central  Google Scholar 

  7. Lopez WO, Nikkhah G, Kahlert UD, Maciaczyk D, Bogiel T, Moellers S, Schültke E, Döbrössy M, Maciaczyk J. Clinical neurotransplantation protocol for Huntington’s and Parkinson’s disease. Restor. Neurol. Neurosci. 2013;31(5):579-595. doi: https://doi.org/10.3233/RNN-130317

    Article  CAS  PubMed  Google Scholar 

  8. Markram H, Toledo-Rodriguez M, Wang Y, Gupta A, Silberberg G, Wu C. Interneurons of the neocortical inhibitory system. Nat. Rev. Neurosci. 2004;5(10):793-807. doi: https://doi.org/10.1038/nrn1519

    Article  CAS  PubMed  Google Scholar 

  9. Müller J, Timmermann A, Henning L, Müller H, Steinhäuser C, Bedner P. Astrocytic GABA accumulation in experimental temporal lobe epilepsy. Front. Neurol. 2020;11:614923. doi: https://doi.org/10.3389/fneur.2020.614923

    Article  PubMed  PubMed Central  Google Scholar 

  10. Namestnikova DD, Cherkashova EA, Sukhinich KK, Gubskiy IL, Leonov GE, Gubsky LV, Majouga AG, Yarygin KN. Combined cell therapy in the treatment of neurological disorders. Biomedicines. 2020;8(12):613. doi: https://doi.org/10.3390/biomedicines8120613

    Article  CAS  PubMed Central  Google Scholar 

  11. Obata K, Hirono M, Kume N, Kawaguchi Y, Itohara S, Yanagawa Y. GABA and synaptic inhibition of mouse cerebellum lacking glutamate decarboxylase 67. Biochem. Biophys. Res. Commun. 2008;370(3):429-433. doi: https://doi.org/10.1016/j.bbrc.2008.03.110

    Article  CAS  PubMed  Google Scholar 

  12. Pekny M, Pekna M. Astrocyte intermediate filaments in CNS pathologies and regeneration. J. Pathol. 2004;204(4):428-437. doi: https://doi.org/10.1002/path.1645

    Article  CAS  PubMed  Google Scholar 

  13. Semyanov A, Walker MC, Kullmann DM, Silver RA. Tonically active GABA A receptors: modulating gain and maintaining the tone. Trends Neurosci. 2004;27(5):262-269. doi: https://doi.org/10.1016/j.tins.2004.03.005

    Article  CAS  PubMed  Google Scholar 

  14. Sequerra EB, Gardino P, Hedin-Pereira C, de Mello FG. Putrescine as an important source of GABA in the postnatal rat subventricular zone. Neuroscience. 2007;146(2):489-493. doi: https://doi.org/10.1016/j.neuroscience.2007.01.062

    Article  CAS  PubMed  Google Scholar 

  15. Zhuravleva ZN. The hippocampus and neurotransplantation. Neurosci Behav Physiol. 2005;35(4):343-354. doi: https://doi.org/10.1007/s11055-005-0031-3

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, Russia

    Z. N. Zhuravleva & S. S. Khutsyan

  2. Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, Russia

    S. S. Khutsyan & G. I. Zhuravlev

Authors
  1. Z. N. Zhuravleva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. S. Khutsyan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. I. Zhuravlev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Z. N. Zhuravleva.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 173, No. 4, pp. 503-507, April, 2022

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhuravleva, Z.N., Khutsyan, S.S. & Zhuravlev, G.I. Ultrastructural Immunocytochemistry of GABAergic Cells in Neocortical Neurotransplants. Bull Exp Biol Med 173, 490–493 (2022). https://doi.org/10.1007/s10517-022-05567-3

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-022-05567-3

Key Words

Search

Navigation