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Effect of Conditioned Media of Limbal Epithelial Cells and Corneal Stromal Cells on Functional Activity of Limb Mesenchymal Stem Cells

  • EXPERIMENTAL BIOLOGY
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Bulletin of Experimental Biology and Medicine Aims and scope

We studied the effect of conditioned media from limbal epithelial stem cells, fibroblasts, and corneal keratocytes on the functional activity of human limbal mesenchymal stem cells. It was shown that the conditioned media from limbal epithelial stem cells reduced proliferative activity and inhibited migration of limbal mesenchymal stem cells. In the conditioned media of limbal epithelial stem cells, increased concentrations of VEGF and TNFα and reduced concentration of BDNF, vimentin, and fibronectin were found. The conditioned medium from corneal stromal cells did not affect functional activity of mesenchymal stem cells in the limbus. These data contribute to the understanding of the interaction of cells in the limbal niche and with corneal cells essential for the maintenance of the cellular homeostasis in the cornea.

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Authors and Affiliations

  1. Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia

    N. A. Bondarenko, M. A. Surovtseva, I. I. Kim, A. P. Lykov & O. V. Poveshchenko

  2. S. N. Fyodorov Eye Microsurgery Federal State Institution of the Russian Ministry of Health — Novosibirsk Branch, Novosibirsk, Russia

    I. A. Iskakov, A. N. Trunov & V. V. Chernykh

Authors
  1. N. A. Bondarenko
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  2. M. A. Surovtseva
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  3. I. I. Kim
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  4. A. P. Lykov
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  5. I. A. Iskakov
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  6. A. N. Trunov
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  7. V. V. Chernykh
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  8. O. V. Poveshchenko
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Corresponding author

Correspondence to N. A. Bondarenko.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 173, No. 4, pp. 474-478, April, 2022

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Bondarenko, N.A., Surovtseva, M.A., Kim, I.I. et al. Effect of Conditioned Media of Limbal Epithelial Cells and Corneal Stromal Cells on Functional Activity of Limb Mesenchymal Stem Cells. Bull Exp Biol Med 173, 464–467 (2022). https://doi.org/10.1007/s10517-022-05562-8

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  • DOI: https://doi.org/10.1007/s10517-022-05562-8

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