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Clinical Diagnostic Value of Dkk-1 Level in Ankylosing Spondylitis: Comparison of Test Systems Based on Aptamers and Antibodies

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Bulletin of Experimental Biology and Medicine Aims and scope

In ankylosing spondylitis, the pathological metabolism of the bone tissue is regulated by various proteins; of these, Dkk-1 protein, an antagonist of the Wnt-signaling pathway, is of particular interest. We compared the methods of Dkk-1 detection in the blood serum of patients with ankylosing spondylitis (conventional ELISA and aptamer/antibody assay) and analyzed the relationship between Dkk-1 level and structural progression of ankylosing spondylitis and secondary osteoporosis. Dkk-1 levels in patients were significantly increased and depended on the stage of the disease, but not on the presence/absence of osteoporosis. Thus, Dkk-1 is a potential serum marker of progression of ankylosing spondylitis reflecting a tendency to structural progression of the disease before the appearance of radiographic changes. The results obtained by both methods practically coincided, which suggests good prospects for applying DNA aptamer-based test system.

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References

  1. Grebennikova TA, Belaya ZhE, Rozhinskaya LYa, Melnichenko GA. The canonical Wnt/β-catenin pathway: from the history of its discovery to clinical application. Ter. Arkhiv. 2016;88(10):74-81. https://doi.org/10.17116/terarkh201688674-81. Russian.

  2. Daoussis D, Andonopoulos AP. The emerging role of Dickkopf-1 in bone biology: is it the main switch controlling bone and joint remodeling? Semin. Arthritis Rheum. 2011;41(2):170-177. https://doi.org/10.1016/j.semarthrit.2011.01.006

    Article  CAS  PubMed  Google Scholar 

  3. Daoussis D, Liossis SN, Solomou EE, Tsanaktsi A, Bounia K, Karampetsou M, Yiannopoulos G, Andonopoulos AP. Evidence that Dkk-1 is dysfunctional in ankylosing spondylitis. Arthritis Rheum. 2010;62(1):150-158. https://doi.org/10.1002/art.27231

    Article  CAS  PubMed  Google Scholar 

  4. Delgado-Calle J, Sato AY, Bellido T. Role and mechanism of action of sclerostin in bone. Bone. 2017;96:29-37. https://doi.org/10.1016/j.bone.2016.10.007

    Article  CAS  PubMed  Google Scholar 

  5. Diarra D, Stolina M, Polzer K, Zwerina J, Ominsky MS, Dwyer D, Korb A, Smolen J, Hoffmann M, Scheinecker C, van der Heide D, Landewe R, Lacey D, Richards WG, Schett G. Dickkopf-1 is a master regulator of joint remodeling. Nat. Med. 2007;13(2):156-163. https://doi.org/10.1038/nm1538

    Article  CAS  PubMed  Google Scholar 

  6. Heiland GR, Appel H, Poddubnyy D, Zwerina J, Hueber A, Haibel H, Baraliakos X, Listing J, Rudwaleit M, Schett G, Sieper J. High level of functional dickkopf-1 predicts protection from syndesmophyte formation in patients with ankylosing spondylitis. Ann. Rheum. Dis. 2012;71(4):572-574. https://doi.org/10.1136/annrheumdis-2011-200216

    Article  CAS  PubMed  Google Scholar 

  7. Klavdianou K, Liossis SN, Daoussis D. Dkk1: a key molecule in joint remodelling and fibrosis. Mediterr. J. Rheumatol. 2017;28(4):174-182. https://doi.org/10.31138/mjr.28.4.174

    Article  PubMed  PubMed Central  Google Scholar 

  8. Liao HT, Lin YF, Tsai CY, Chou TC. Bone morphogenetic proteins and Dickkopf-1 in ankylosing spondylitis. Scand. J. Rheumatol. 2018;47(1):56-61. https://doi.org/10.1080/03009742.2017.1287305

    Article  CAS  PubMed  Google Scholar 

  9. Nocturne G, Pavy S, Boudaoud S, Seror R, Goupille P, Chanson P, van der Heijde D, van Gaalen F, Berenbaum F, Mariette X, Briot K, Feydy A, Claudepierre P, Dieudé P, Nithitham J, Taylor KE, Criswell LA, Dougados M, Roux C, Miceli-Richard C. Increase in Dickkopf-1 serum level in recent spondyloarthritis. Data from the DESIR Cohort. PLoS One. 2015;10(8):e0134974. https://doi.org/10.1371/journal.pone.0134974

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Pedersen SJ, Maksymowych WP. The pathogenesis of ankylosing spondylitis: an update. Curr. Rheumatol. Rep. 2019;21(10):58. https://doi.org/10.1007/s11926-019-0856-3

    Article  CAS  PubMed  Google Scholar 

  11. Rossini M, Viapiana O, Idolazzi L, Ghellere F, Fracassi E, Troplini S, Povino MR, Kunnathully V, Adami S, Gatti D. Higher level of Dickkopf-1 is associated with low bone mineral density and higher prevalence of vertebral fractures in patients with ankylosing spondylitis. Calcif. Tissue Int. 2016;98(5):438-445. https://doi.org/10.1007/s00223-015-0093-3

    Article  CAS  PubMed  Google Scholar 

  12. Shatunova EA, Korolev MA, Omelchenko VO, Kurochkina YD, Davydova AS, Venyaminova AG, Vorobyeva MA. Aptamers for proteins associated with rheumatic diseases: progress, challenges, and prospects of diagnostic and therapeutic applications. Biomedicines. 2020;8(11):527. https://doi.org/10.3390/biomedicines8110527

    Article  CAS  PubMed Central  Google Scholar 

  13. Walsh MC, Choi Y. Biology of the RANKL-RANK-OPG system in immunity, bone, and beyond. Front. Immunol. 2014;5:511. https://doi.org/10.3389/fimmu.2014.00511

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Xiong JH, Liu J, Chen J. Clinical significance and prognostic value of tumor necrosis factor-α and dickkopf related protein-1 in ankylosing spondylitis. World J. Clin. Cases. 2020;8(7):1213-1222. https://doi.org/10.12998/wjcc.v8.i7.1213

    Article  PubMed  PubMed Central  Google Scholar 

  15. Zhou Y, Li W, Tseng Y, Zhang J, Liu J. Developing slow-off dickkopf-1 aptamers for early-diagnosis of hepatocellular carcinoma. Talanta. 2019;194:422-429. https://doi.org/10.1016/j.talanta.2018.10.014

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia

    M. A. Vorobyeva & E. A. Shatunova

  2. Research Institute of Clinical and Experimental Lymphology — Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia

    K. I. Kolpakov, Yu. D. Kurochkina & M. A. Korolev

Authors
  1. M. A. Vorobyeva
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  2. E. A. Shatunova
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  3. K. I. Kolpakov
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  4. Yu. D. Kurochkina
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  5. M. A. Korolev
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Corresponding author

Correspondence to M. A. Korolev.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 173, No. 3, pp. 307-312, March, 2022

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Vorobyeva, M.A., Shatunova, E.A., Kolpakov, K.I. et al. Clinical Diagnostic Value of Dkk-1 Level in Ankylosing Spondylitis: Comparison of Test Systems Based on Aptamers and Antibodies. Bull Exp Biol Med 173, 317–321 (2022). https://doi.org/10.1007/s10517-022-05541-z

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  • DOI: https://doi.org/10.1007/s10517-022-05541-z

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