Syringopicroside is a kind of iridoid monomer compound isolated from Syringa oblata exhibiting a potent effect against hepatitis B virus (HBV). The therapeutic effect and safety of syringopicroside-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles (SYR-NP) were studied on the model of HBV-infected ducklings and on cultured HepG2.2.15 cells. HBV DNA in ducklings was assessed by fluorescence quantitative PCR. In HepG2.2.15 cells, the content of HBsAg and HBeAg were assayed. Acute toxicity of SYR-NP was studied in ICR mice in 12 h and 7 days after SYR-NP administration. The serum levels of HBV DNA in ducklings treated with SYR-NP in a high dose was significantly lower than in the control. In HepG2.2.15 cells treated with different doses of SYR-NP, the concentrations of HBsAg and HBeAg were significantly below the control. Acute toxicity test showed high safety of SYR-NP. Thus, SYR-NP can inhibit replication of HBV DNA and protect the liver tissue.
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Cui L, Hu M, Cao P, Niu Y, Li C, Liu Z, Kang W. Chemical constituents and coagulation activity of Syringa oblata Lindl flowers. BMC Chem. 2019;13(1):108. doi: https://doi.org/10.1186/s13065-019-0621-8
French J, Locarnini S, Zoulim F. Direct-acting antivirals and viral RNA targeting for hepatitis B cure. Curr. Opin. HIV AIDS. 2020;15(3):165-172. doi: https://doi.org/10.1097/COH.0000000000000622
Ginzberg D, Wong RJ, Gish R. Global HBV burden: guesstimates and facts. Hepatol. Int. 2018;12(4):315-329. doi:https://doi.org/10.1007/s12072-018-9884-8
Guan Q, Sun S, Li X, Lv S, Xu T, Sun J, Feng W, Zhang L, Li Y. Preparation, in vitro and in vivo evaluation of mPEG-PLGA nanoparticles co-loaded with syringopicroside and hydroxytyrosol. J. Mater Sci. Mater Med. 2016;27(2):24. doi: https://doi.org/10.1007/s10856-015-5641-x
Li YJ, Ma MT, Lv SW, Zhao JH. Separation of syringin A by macroporous adsorption resin. J. Tradit. Chin. Med. 2004;(1):16-17. doi: https://doi.org/10.19664/j.cnki.1002-2392.2004.01.009
Nenadis N, Vervoort J, Boeren S, Tsimidou MZ. Syringa oblata Lindl var. alba as a source of oleuropein and related compounds. J. Sci. Food Agric. 2010;87(1):160-166. doi: https://doi.org/10.1002/jsfa.2697
Nguyen MH, Wong G, Gane E, Kao JH, Dusheiko G. Hepatitis B Virus: Advances in Prevention, Diagnosis, and Therapy. Clin. Microbiol. Rev. 2020;33(2):e00046-19. doi: https://doi.org/10.1128/CMR.00046-19
Shaw T, Locarnini SA, Luscombe CA. Chapter 123 – Animal Models for Hepatitis B Infections — Duck Hepatitis. Handbook of Animal Models of Infection. Experimental Models in Antimicrobial Chemotherapy. Elsevier, 1999. P. 1021-1031. doi: https://doi.org/10.1016/B978-012775390-4/50262-1
Tagawa M, Yokosuka O, Imazeki F, Ohto M, Omata M. Gene expression and active virus replication in the liver after injection of duck hepatitis B virus DNA into the peripheral vein of ducklings. J. Hepatol. 1996;24(3):328-334. doi: https://doi.org/10.1016/s0168-8278(96)80013-4
Yan J, Xie W, Wang Q, Li Y, Feng X, Cheng J. The optimal threshold: Baseline serum hepatitis B virus DNA and alanine transaminase levels can predict the 2-Year on-treatment virological response to lamivudine. Hepat. Mon. 2011;11(5):358-363.
Yang H, Zhou Z, He L, Ma H, Qu W, Yin J, Jia M, Zhao X, Shan J, Gao Y. Hepatoprotective and inhibiting HBV effects of polysaccharides from roots of Sophora flavescens. Int. J. Biol. Macromol. 2018;108:744-752. doi: https://doi.org/10.1016/j.ijbiomac.2017.10.171
Yang N, Bertoletti A. Advances in therapeutics for chronic hepatitis B. Hepatol. Int. 2016;10(2):277-285. doi: https://doi.org/10.1007/s12072-015-9661-x
Zhang X, Lü S, Han J, Sun S, Wang L, Li Y. Preparation, characterization and in vivo distribution of solid lipid nanoparticles loaded with syringopicroside. Pharmazie. 2011;66(6):404-407.
Zhang Y, Wang X, Pang G, Cai F, Zhang J, Shen Z, Li R, Shen Q. Two-step genomic sequence comparison strategy to design Trichoderma strain-specific primers for quantitative PCR. AMB Express. 2019;9(1):179. doi: https://doi.org/10.1186/s13568-019-0904-4
Zhao G, Yin Z, Dong J. Antiviral efficacy against hepatitis B virus replication of oleuropein isolated from Jasminum officinale L. var. grandiflorum. J. Ethnopharmacol. 2009;125(2):265-268. doi: https://doi.org/10.1016/j.jep.2009.06.030
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 172, No. 11, pp. 611-617, November, 2021
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Zhang, X.W., Li, M.C. & Dou, J.J. Effect of Hepatocyte Targeting Nanopreparation Syringopicroside on Duck Hepatitis B Virus and Evaluation of Its Safety. Bull Exp Biol Med 172, 573–578 (2022). https://doi.org/10.1007/s10517-022-05436-z
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DOI: https://doi.org/10.1007/s10517-022-05436-z