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The Role of Redox Status Changes in Dexamethasone-Induced Apoptosis in Jurkat Tumor Cells

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The apoptotic death and its regulation was studied in intact Jurkat tumor cells and under the influence of buthionine-sulfoximine (de novo glutathione synthesis inhibitor; 1 mM) and/or apoptosis inducer dexamethasone (10 μM). The role of glutathione system components in dexamethasone-induced apoptosis in Jurkat tumor cells (both receptor-mediated and mitochondrial pathways) was analyzed. Under conditions of dexamethasone-induced apoptosis, glutathione system blockage mostly affects presentation of TNF RI- and Fas-receptors in Jurkat tumor cells, as well as change in content of transcription factors Apaf-1 and NF-κB, thereby promoting cell death. The decrease in the content of oxidized glutathione produced a potentiating effect on dexamethasone-induced apoptotic death of Jurkat tumor cells.

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References

  1. Nosareva OL, Stepovaya EA, Ryazantseva NV, Shakhristova EV, Orlov DS, Novitsky VV. Ubiquitin and regulation of apoptosis in Jurkat cells. Byull. Sib. Med. 2018;17(3):96-104. doi: https://doi.org/10.20538/1682-0363-2018-3-96-104. Russian.

  2. Nosareva OL, Stepovaya EA, Shakhristova EV, Alekseeva ON, Kuzmenko DI, Sadykova AA, Novitsky VV. The Role of Redox Status and Oxidative Modification of Proteins in Implementing Apoptosis in Human Blood Lymphocytes in Norm and Under Experimental Oxidative Stress. Russ. Zh. Fiziol. 105(3):327-338. doi: https://doi.org/10.1134/S0869813919030063

  3. Corso CR, Acco A. Glutathione system in animal model of solid tumors: From regulation to therapeutic target. Crit. Rev. Oncol. Hematol. 2018;128:43-57. doi: https://doi.org/10.1016/j.critrevonc.2018.05.014

    Article  PubMed  Google Scholar 

  4. Dominko K, Đikić D. Glutathionylation: a regulatory role of glutathione in physiological processes. Arh. Hig. Rada Toksikol. 2018;69(1):1-24. doi: https://doi.org/10.2478/aiht-2018-69-2966

    Article  CAS  PubMed  Google Scholar 

  5. Harris IS, Treloar AE, Inoue S, Sasaki M, Gorrini C, Lee KC, Yung KY, Brenner D, Knobbe-Thomsen CB, Cox MA, Elia A, Berger T, Cescon DW, Adeoye A, Brüstle A, Molyneux SD, Mason JM, Li WY, Yamamoto K, Wakeham A, Berman HK, Khokha R, Done SJ, Kavanagh TJ, Lam CW, Mak TW. Glutathione and thioredoxin antioxidant pathways synergize to drive cancer initiation and progression. Cancer Cell. 2015;27(2):211- 222. doi: https://doi.org/10.1016/j.ccell.2014.11.019

    Article  CAS  PubMed  Google Scholar 

  6. Kesarwani P, Murali AK, Al-Khami AA, Mehrotra S. Redox regulation of T-cell function: from molecular mechanisms to significance in human health and disease. Antioxid. Redox Signal. 2013;18(12):1497-1534. doi: https://doi.org/10.1089/ars.2011.4073

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Laragione T, Bonetto V, Casoni F, Massignan T, Bianchi G, Gianazza E, Ghezzi P. Redox regulation of surface protein thiols: identification of integrin alpha-4 as a molecular target by using redox proteomics. Proc. Natl Acad. Sci. USA. 2003 Vol. 100(25):14737-14741. doi: https://doi.org/10.1073/pnas.2434516100

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Manda G, Isvoranu G, Comanescu MV, Manea A, Debelec Butuner B, Korkmaz KS. The redox biology network in cancer pathophysiology and therapeutics. Redox Biol. 2015;5:347- 357. doi: https://doi.org/10.1016/j.redox.2015.06.014

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Nosareva OL, Stepovaya EA, Ryazantseva NV, Shakhristova EV, Egorova MY, Novitsky VV. The role of the glutathione system in oxidative modification of proteins and dysregulation of apoptosis in Jurkat tumor cells. Bull. Exp. Biol. Med. 2017;164(2):199-202. doi: https://doi.org/10.1007/s10517-017-3957-x

    Article  CAS  PubMed  Google Scholar 

  10. Schoeneberger H, Belz K, Schenk B, Fulda S. Impairment of antioxidant defense via glutathione depletion sensitizes acute lymphoblastic leukemia cells for Smac mimetic-induced cell death. Oncogene. 2015;34(31):4032-4043. doi: https://doi.org/10.1038/onc.2014.338

    Article  CAS  PubMed  Google Scholar 

  11. Teskey G, Abrahem R, Cao R, Gyurjian K, Islamoglu H, Lucero M, Martinez A, Paredes E, Salaiz O, Robinson B, Venketaraman V. Glutathione as a marker for human disease. Adv. Clin. Chem. 2018;87:141-159. doi: https://doi.org/10.1016/bs.acc.2018.07.004

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia

    О. L. Nosareva, E. A. Stepovaya, E. V. Shakhristova & R. M. Karpov

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  1. О. L. Nosareva
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  2. E. A. Stepovaya
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  3. E. V. Shakhristova
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  4. R. M. Karpov
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Correspondence to О. L. Nosareva.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 172, No. 10, pp. 479-482, October, 2021

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Nosareva, О.L., Stepovaya, E.A., Shakhristova, E.V. et al. The Role of Redox Status Changes in Dexamethasone-Induced Apoptosis in Jurkat Tumor Cells. Bull Exp Biol Med 172, 464–466 (2022). https://doi.org/10.1007/s10517-022-05414-5

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  • DOI: https://doi.org/10.1007/s10517-022-05414-5

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