Skip to main content
SpringerLink
Log in

Comparative Proteomic Analysis of the Mycoplasma gallisepticum Nucleoid Fraction before and after Infection

  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Mycoplasma gallisepticum belongs to the class Mollicutes and induces severe chronic respiratory disease in chickens. It lacks the cell wall and contains a very small genome and, accordingly, a reduced set of regulatory proteins. It is assumed that one of the regulatory mechanisms in mycoplasmas may be the dynamics of the spatial organization of the chromosome. M. gallisepticum has only two known nucleoid-associated (NAP) histone-like proteins (Hup_1 and Hup_2). To search for new potential NAP that may play a role in the infection process, we isolated nucleoid fractions from M. gallisepticum cells before and after infection of HD3 chicken erythroblast cell line and performed a comparative proteomic analysis of these fractions. We identified several potential NAP that included the components of the terminal organelle and adhesion, VlhA antigen, NADH oxidase, and PykF pyruvate kinase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Chen H, Yu S, Shen X, Chen D, Qiu X, Song C, Ding C. The Mycoplasma gallisepticum α-enolase is cell surface-exposed and mediates adherence by binding to chicken plasminogen. Microb. Pathog. 2011;51(4):285-290. https://doi.org/10.1016/j.micpath.2011.03.012

    Article  CAS  PubMed  Google Scholar 

  2. Fisunov GY, Garanina IA, Evsyutina DV, Semashko TA, Nikitina AS, Govorun VM. Reconstruction of transcription control networks in mollicutes by high-throughput identification of promoters. Front. Microbiol. 2016;7:1977. https://doi.org/10.3389/fmicb.2016.01977

    Article  PubMed  PubMed Central  Google Scholar 

  3. Lai WKM, Pugh BF. Understanding nucleosome dynamics and their links to gene expression and DNA replication. Nat. Rev. Mol. Cell Biol. 2017;18(9):548-562. https://doi.org/10.1038/nrm.2017.47

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Lazarev VN, Parfenova TM, Gularyan SK, Misyurina OY, Akopian TA, Govorun VM. Induced expression of melittin, an antimicrobial peptide, inhibits infection by Chlamydia trachomatis and Mycoplasma hominis in a HeLa cell line. Int. J. Antimicrob. Agents. 2002;19(2):133-137. https://doi.org/10.1016/s0924-8579(01)00479-4

    Article  CAS  PubMed  Google Scholar 

  5. Matyushkina D, Pobeguts O, Butenko I, Vanyushkina A, Anikanov N, Bukato O, Evsyutina D, Bogomazova A, Lagarkova M, Semashko T, Garanina I, Babenko V, Vakhitova M, Ladygina V, Fisunov G, Govorun V. Phase transition of the bacterium upon invasion of a host cell as a mechanism of adaptation: a Mycoplasma gallisepticum model. Sci. Rep. 2016;6:35959. https://doi.org/10.1038/srep35959

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Pflaum K, Tulman ER, Beaudet J, Canter J, Geary SJ. Variable lipoprotein hemagglutinin A gene (vlhA) expression in variant Mycoplasma gallisepticum strains in vivo. Infect. Immun. 2018;86(11):e00524-18. https://doi.org/10.1128/IAI.00524-18

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Pobeguts OV, Ladygina VG, Evsyutina DV, Eremeev AV, Zubov AI, Matyushkina DS, Scherbakov PL, Rakitina DV, Fisunov GY. Propionate induces virulent properties of Crohn’s disease-associated Escherichia coli. Front. Microbiol. 2020;11:1460. https://doi.org/10.3389/fmicb.2020.01460

    Article  PubMed  PubMed Central  Google Scholar 

  8. Rigden DJ, Setlow P, Setlow B, Bagyan I, Stein RA, Jedrzejas MJ. PrfA protein of Bacillus species: prediction and demonstration of endonuclease activity on DNA. Protein Sci. 2002;11(10):2370-2381. https://doi.org/10.1110/ps.0216802

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Seto S, Layh-Schmitt G, Kenri T, Miyata M. Visualization of the attachment organelle and cytadherence proteins of Mycoplasma pneumoniae by immunofluorescence microscopy. J. Bacteriol. 2001;183(5):1621-1630. https://doi.org/10.1128/JB.183.5.1621-1630.2001

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Subramanian A, Miller DM. Structural analysis of alphaenolase. Mapping the functional domains involved in downregulation of the c-myc protooncogene. J. Biol. Chem. 2000;275(8):5958-5965. https://doi.org/10.1074/jbc.275.8.5958

    Article  CAS  PubMed  Google Scholar 

  11. Trussart M, Yus E, Martinez S, Baù D, Tahara YO, Pengo T, Widjaja M, Kretschmer S, Swoger J, Djordjevic S, Turnbull L, Whitchurch C, Miyata M, Marti-Renom MA, Lluch-Senar M, Serrano L. Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae. Nat. Commun. 2017;8:14665. https://doi.org/10.1038/ncomms14665

    Article  PubMed  PubMed Central  Google Scholar 

  12. Xayarath B, Volz KW, Smart JI, Freitag NE. Probing the role of protein surface charge in the activation of PrfA, the central regulator of Listeria monocytogenes pathogenesis. PLoS One. 2011;6(8):e23502. https://doi.org/10.1371/journal.pone.0023502

  13. Zgoda V, Tikhonova O, Viglinskaya A, Serebriakova M, Lisitsa A, Archakov A. Proteomic profiles of induced hepatotoxicity at the subcellular level. Proteomics. 2006;6(16):4662-4670. https://doi.org/10.1002/pmic.200600342

    Article  CAS  PubMed  Google Scholar 

  14. Zubov AI, Semashko TA, Evsyutina DV, Ladygina VG, Kovalchuk SI, Ziganshin RH, Galyamina MA, Fisunov GY, Pobeguts OV. Data on nucleoid-associated proteins isolated from Mycoplasma Gallisepticum in different growth phases. Data Brief. 2020;31:105853. https://doi.org/10.1016/j.dib.2020.105853

Download references

Author information

Authors and Affiliations

  1. Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical-Biological Agency of Russia, Moscow, Russia

    M. A. Galyamina, A. I. Zubov, V. G. Ladygina, A. V. Li, D. S. Matyushkina, O. V. Pobeguts & G. Yu. Fisunov

Authors
  1. M. A. Galyamina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. I. Zubov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. G. Ladygina
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. V. Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. D. S. Matyushkina
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. O. V. Pobeguts
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. G. Yu. Fisunov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. A. Galyamina.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 172, No. 9, pp. 328-333, September, 2021

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Galyamina, M.A., Zubov, A.I., Ladygina, V.G. et al. Comparative Proteomic Analysis of the Mycoplasma gallisepticum Nucleoid Fraction before and after Infection. Bull Exp Biol Med 172, 336–340 (2022). https://doi.org/10.1007/s10517-022-05388-4

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-022-05388-4

Key Words

Search

Navigation