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Morphofunctional Properties of Corneal Stromal Cells

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Bulletin of Experimental Biology and Medicine Aims and scope

Human corneal stromal cells were isolated by enzymatic digestion from a new source, lenticules obtained during laser vision correction by the ReLEx SMILe method. The resulting culture was mainly presented by fibroblast-like cells with a phenotype CD90—/CD73+/CD105+/keratocan—/lumican—/ALDH1A1+ that differentiate into keratocytes in a specialized medium. The concentration of fetal calf serum-derived growth factors affects the rate of proliferation, production of erythropoietin and brain neurotrophic factor by corneal fibroblasts, and to a lesser extent, their migration activity and production of extracellular matrix components. Thus, the high functional potential of fibroblast-like cells isolated from stromal lenticles can be used to develop cell technologies in ophthalmology.

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Authors and Affiliations

  1. Research Institute of Clinical and Experimental Lymphology — Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia

    M. A. Surovtseva, O. V. Poveshchenko, I. I. Kim, A. P. Lykov, N. A. Bondarenko & L. A. Shul’mina

  2. S. N. Fyodorov Eye Microsurgery Federal State Institution of the Russian Ministry of Health, Novosibirsk, Russia

    K. Yu. Krasner, A. N. Trunov & V. V. Chernykh

Authors
  1. M. A. Surovtseva
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  2. O. V. Poveshchenko
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  3. K. Yu. Krasner
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  4. I. I. Kim
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  5. A. P. Lykov
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  6. N. A. Bondarenko
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  7. L. A. Shul’mina
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  8. A. N. Trunov
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  9. V. V. Chernykh
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Correspondence to M. A. Surovtseva.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 172, No. 7, pp. 115-119, July, 2021

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Surovtseva, M.A., Poveshchenko, O.V., Krasner, K.Y. et al. Morphofunctional Properties of Corneal Stromal Cells. Bull Exp Biol Med 172, 96–99 (2021). https://doi.org/10.1007/s10517-021-05339-5

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  • DOI: https://doi.org/10.1007/s10517-021-05339-5

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