We studied the effect of tilorone on the dynamics of IFNα, IFNγ, and IL-1β levels in the lung tissue and blood serum in relation to viral load in the lungs of BALB/c mice with pneumonia caused by influenza virus A/Aichi/2/68 (H3N2). Tilorone was administered per os in doses of 40, 150, and 540 μg per mouse 6, 30, and 78 h postinfection, which simulated the drug regimen used in the clinic for the treatment of influenza and acute respiratory viral infections in Russia and post-Soviet countries. Tilorone reduced viral load with the maximum amplitude (2-3 lg) after 1-2 administrations. The results of studying the dynamics of the cytokine levels in the infected animals in general support the previous hypothesis that, in repeated dosing, tilorone enhances the IFN response (compensates for its deficiency) at the early stages of acute respiratory viral infections and suppresses (damps) excessive production of IFN and proinflammatory cytokines at the later stages.
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Grigoryan SS, Isaeva EI, Bakalov VV, Oispova EA, Bevz AYu, Prostyakov IV, Nadorov SA. Amixin – induction of interferons alpha, beta, gamma and lambda in blood serum and pulmonary tissue. Russ, Med, Zh. Med. Oozrenie. 2015;(2):93-99. Russian.
Grigoryan SS, Kovalenko AL, Isaeva EI, Vetrova EN, Tyusheva VV. Cycloferon and influenza a (H3N2) virus in cell cultures: effect of cycloferon on H3N2 virus reproduction in cells pretreated before infection. Eksp. Klin. Farmakol. 2018;81(11):26-31. doi: 10.30906/0869-2092-2018-81-11-26-31. Russian.
Kalyuzhin OV. Acute respiratory viral infections: modern challenges, a fresh approach to the place of interferon inductors in the prevention and therapy. Lech. Vrach. 2013;(9):78-84.Russian.
Kalyuzhin OV. Tilorone as a chosen preparation for prevention and treatment of acute respiratory viral infections Lech. Vrach. 2013;(10):43-48. Russian.
Ekins S, Lane TR, Madrid PB. Tilorone: a broad-spectrum antiviral invented in the USA and commercialized in Russia and beyond. Pharm. Res. 2020;37(4):71. doi: https://doi.org/10.1007/s11095-020-02799-8
Ekins S, Madrid PB. Tilorone, a broad-spectrum antiviral for emerging viruses. Antimicrob. Agents Chemother. 2020;64(5):e00440-20. doi: https://doi.org/10.1128/AAC.00440-20
Krueger RE, Mayer GD. Tilorone hydrochloride: an orally active antiviral agent. Science. 1970;169:1213-1214. doi: https://doi.org/10.1126/science.169.3951.1213
Nair AB, Jacob S. A simple practice guide for dose conversion between animals and human. J. Basic Clin. Pharm. 2016;7(2):27-31. doi: 10.4103/0976-0105.177703
Stringfellow DA. Production of the interferon protein: hyporesponsiveness. Tex. Rep. Biol. Med. 1977;35:126-131.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 171, No. 6, pp. 724-728, June, 2021
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Kalyuzhin, O.V., Isaeva, E.I., Vetrova, E.N. et al. Effect of Tilorone on the Dynamics of Viral Load and the Levels of Interferons and Interleukin-1β in the Lung Tissue and Blood Serum of Mice with Experimental Influenza. Bull Exp Biol Med 171, 736–740 (2021). https://doi.org/10.1007/s10517-021-05306-0
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DOI: https://doi.org/10.1007/s10517-021-05306-0