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The Effect of TLR Agonists and Myokines on Secretory Activity of Adipogenically Differentiated MSC Cultures

  • BIOPHYSICS AND BIOCHEMISTRY
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Bulletin of Experimental Biology and Medicine Aims and scope

We studied the effect of bacterial pathogen-associated molecular patterns and myokines on the secretion of adipokines by mesenchymal stem cells (MSC) and products of their adipogenic differentiation. The secretion of adiponectin, adipsin, leptin, and insulin by adipogenically differentiated cell cultures was quantitatively determined using multiplex ELISA. MSC obtained from the stromal vascular fraction of human subcutaneous adipose tissue were shown to secrete a known adipokine adipsin. The ability of white adipocytes to secrete significant amounts of insulin (in vitro) has been shown for the first time. Control cultures of white adipocytes secreted much higher levels of adiponectin, leptin, and insulin when compared to other adipocytes cultures. On the other hand, beige and brown adipocyte cultures secreted more adipsin than white adipocyte cultures. The influence of myokine β-aminoisobutyric acid on the secretion of adipsin in MSC, white, beige, and brown adipocytes was also studied.

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Authors and Affiliations

  1. N. I. Pirogov Russian National Research Medical University, Ministry of the Health of the Russian Federation, Moscow, Russia

    A. V. Shestopalov & S. A. Rumyantsev

  2. Center of Digital and Translational Biomedicine, LLC “Center for Molecular Health”, Moscow, Russia

    A. V. Shestopalov, A. M. Gaponov & S. A. Rumyantsev

  3. Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Moscow region, Russia

    A. Mishra

Authors
  1. A. V. Shestopalov
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  2. A. Mishra
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  3. A. M. Gaponov
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  4. S. A. Rumyantsev
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Corresponding author

Correspondence to A. Mishra.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 171, No. 6, pp. 708-713, June, 2021

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Shestopalov, A.V., Mishra, A., Gaponov, A.M. et al. The Effect of TLR Agonists and Myokines on Secretory Activity of Adipogenically Differentiated MSC Cultures. Bull Exp Biol Med 171, 722–726 (2021). https://doi.org/10.1007/s10517-021-05303-3

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  • DOI: https://doi.org/10.1007/s10517-021-05303-3

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