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Effect of Detergents on Activity and Magnesium-Dependent Properties of Different Isoforms of Na+,K+-ATPase in the Crude Membrane Fraction of Rat Cerebral Cortex

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We studied the effect of various detergents (Tween-20, Triton X-100, and sodium deoxycholate) on activity and magnesium-dependent properties of Na+,K+-ATPase of the crude membrane fraction of rat cerebral cortex. All studied detergents significantly increased activity of the studied enzyme in a concentration-dependent manner. Sodium deoxycholate provided significantly higher values Na+,K+-ATPase activity (by ≈50%) than Triton X-100 and Tween-20. In the presence of Triton X-100, a changed pattern of the dependence of enzyme activity on the concentration of magnesium ions in the incubation solution was noted. Separate measurement of activities of Na+,K+-ATPase isoforms made it possible to assume that changes in magnesium-dependent properties are due to the predominant effect of Triton X-100 on ouabain-sensitive α2- and α3-isoforms.

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Authors and Affiliations

  1. Tyumen State University, Tyumen, Russia

    V. N. Dubrovskii & L. A. Orlova

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  1. V. N. Dubrovskii
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  2. L. A. Orlova
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Correspondence to V. N. Dubrovskii.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 171, No. 5, pp. 583-587, May, 2021

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Dubrovskii, V.N., Orlova, L.A. Effect of Detergents on Activity and Magnesium-Dependent Properties of Different Isoforms of Na+,K+-ATPase in the Crude Membrane Fraction of Rat Cerebral Cortex. Bull Exp Biol Med 171, 611–614 (2021). https://doi.org/10.1007/s10517-021-05279-0

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  • DOI: https://doi.org/10.1007/s10517-021-05279-0

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