There are individual differences in the tolerance to hypoxia and stress. Stress can contribute to the development of various diseases, including inflammatory bowel disease. It was found that inflammatory bowel diseases in animals susceptible to hypoxia runs more severe course than in tolerant animals. We studied morphofunctional changes in the colon under conditions of modeled cold stress in male C57BL/6 mice susceptible and tolerant to hypoxia. The animals were daily subjected to cold stress (20 min at -20°C) for 2 weeks. Cold stress was followed by an increase in the volume fraction of goblet cells in the colon and production of mucins by these cells in mice tolerant to hypoxia and an increase in cell content in the lamina propria of the colon mucous membrane in animals susceptible to hypoxia. The number of serotoninproducing endocrine cells increased in both groups, but these changes were more pronounced in mice susceptible to hypoxia.
Similar content being viewed by others
References
Abdulaeva SO. Morphological characteristics of the intestinal mucose layer and the change of microflora in BALB\C mice during adaptation to cold exposure. Morfol. Vedomosti. 2011;(2):9-13. Russian.
Dzhalilova DSh, Zolotova NA, Polyakova MA, Diatroptov ME, Dobrynina MT, Makarova OV. Morphological features of the inflammatory process and subpopulation pattern of peripheral blood lymphocytes during chronic colitis in mice exhibiting different responses to hypoxia // Klin. Eksper. Morfol. 2018;28(4):13-20. doi: https://doi.org/10.31088/2226-5988-2018-28-4-13-20. Russian.
Dzhalilova DS, Kosyreva AM, Diatroptov ME, Ponomarenko EA, Tsvetkov IS, Zolotova NA, Mkhitarov VA, Khochanskiy DN, Makarova OV. Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats. J. Inflamm. Res. 2019;12:73-86. doi: https://doi.org/10.2147/JIR.S194581
Ebner K, Singewald N. Individual differences in stress susceptibility and stress inhibitory mechanisms. Curr. Opin. Behav. Sci. 2017;14:54-64.doi: https://doi.org/10.1016/j.cobeha.2016.11.016
Fink G. Selye’s general adaptation syndrome: stress-induced gastro-duodenal ulceration and inflammatory bowel disease. J. Endocrinol. 2017;232(3):F1-F5. doi: https://doi.org/10.1530/JOE-16-0547
Gao X, Cao Q, Cheng Y, Zhao D, Wang Z, Yang H, Wu Q, You L, Wang Y, Lin Y, Li X, Wang Y, Bian JS, Sun D, Kong L, Birnbaumer L, Yang Y. Chronic stress promotes colitis by disturbing the gut microbiota and triggering immune system response. Proc. Natl Acad. Sci. USA. 2018;115(13):E2960-E2969. doi: https://doi.org/10.1073/pnas.1720696115
Gunawardene AR, Corfe BM, Staton CA. Classification and functions of enteroendocrine cells of the lower gastrointestinal tract. Int. J. Exp. Pathol. 2011;92(4):219-231. doi: https://doi.org/10.1111/j.1365-2613.2011.00767.x
Habiyambere BM, Onyango E. Chronic stress modulates the mucin components of the intestinal barrier and the intestinal morphology. J. Adv. Med. Med. Res. 2016;13(6):1-14. doi:https://doi.org/10.9734/BJMMR/2016/23764
Jain K, Suryakumar G, Prasad R, Ganju L. Upregulation of cytoprotective defense mechanisms and hypoxia-responsive proteins imparts tolerance to acute hypobaric hypoxia. High Alt. Med. Biol. 2013;14(1):65-77. doi: https://doi.org/10.1089/ham.2012.1064
Julio-Pieper M, O’Mahony CM, Clarke G, Bravo JA, Dinan TG, Cryan JF. Chronic stress-induced alterations in mouse colonic 5-HT and defecation responses are strain dependent. Stress. 2012;15(2):218-226. doi: https://doi.org/10.3109/10253890.2011.607524
Lambert GP. Stress-induced gastrointestinal barrier dysfunction and its inflammatory effects. J. Anim. Sci. 2009;87(14, Suppl):E101-E108. doi: https://doi.org/10.2527/jas.2008-1339
Lukyanova LD, Kirova YI. Effect of hypoxic preconditioning on free radical processes in tissues of rats with different resistance to hypoxia. Bull. Exp. Biol. Med. 2011;151(3):292-296. doi: https://doi.org/10.1007/s10517-011-1312-1
Machorro-Rojas N, Sainz-Espuñes T, Godínez-Victoria M, Castañeda-Sánchez JI, Campos-Rodríguez R, Pacheco-Yepez J, Drago-Serrano ME. Impact of chronic immobilization stress on parameters of colonic homeostasis in BALB/c mice. Mol. Med. Rep. 2019;20(3):2083-2090. doi: https://doi.org/10.3892/mmr.2019.10437
Sesti-Costa R, Ignacchiti MD, Chedraoui-Silva S, Marchi LF, Mantovani B. Chronic cold stress in mice induces a regulatory phenotype in macrophages: correlation with increased 11β-hydroxysteroid dehydrogenase expression. Brain Behav. Immun. 2012;26(1):50-60. doi: https://doi.org/10.1016/j.bbi.2011.07.234
Sgambato D, Miranda A, Ranaldo R, Federico A, Romano M. The role of stress in inflammatory bowel diseases. Curr. Pharm. Des. 2017;23(27):3997-4002. doi: https://doi.org/10.2174/1381612823666170228123357
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 171, No. 4, pp. 519-524, April, 2021
Rights and permissions
About this article
Cite this article
Zolotova, N.A., Dzhalilova, D.S., Khochanskiy, D.N. et al. Morphofunctional Changes in Colon after Cold Stress in Male C57BL/6 Mice Susceptible and Tolerant to Hypoxia. Bull Exp Biol Med 171, 499–503 (2021). https://doi.org/10.1007/s10517-021-05259-4
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-021-05259-4